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About Asano Active Enzyme Molecule Project

directorAsano_photo

 Enzymes are catalytic proteins with high reaction specificity, substrate specificity, and/or enantioselectivity. Enzymes are now used in various fields, such as chemical, food, and pharmaceutical industries, because they exhibit high activity under mild conditions and contribute to reducing environmental pollution and squandered resources. Among these applications, utilization of enzymes in the bioprocesses for the synthesis of useful chemicals or fine chemicals, generally known as "white biotechnology", has become one of the most important technologies in the world in the 21st century.
  To further expand the application of enzymes in "white biotechnology", we are conducting interdisciplinary research by using newly isolated enzymes from not only microorganisms but also plants and animals, and developing artificial metabolism for synthesis of useful chemicals. Our research project also clarifies the relationship between the primary structure of proteins and formation of correct protein folding with enzyme activity in heterologous expression systems. Dynamic structure analysis of proteins and creation of new enzyme functions by modification are also being investigated in our research project. Furthermore, we are developing new enzymatic methods for selective assay of amino acids which are monitored in blood amino acid levels for diagnosis of various diseases.
  These fundamental studies will significantly contribute to the development of "advanced white biotechnology" and human healthcare in the 21st century.

News & Topics

News

The Fifth International Conference on Cofactors & Active Enzyme Molecule 2016

Call for Papers & Registration is open!

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The Fifth International Conference on Cofactors & Active Enzyme Molecule 2016, an international conference, will be held in Toyama, Japan, during September 4-8, 2016 hosted by Conference Chairs Hideyuki Hayashi(Osaka Medical College), and Yasuhisa Asano, Research Director of Asano Active Enzyme Molecule Project, ERATO,JST.
We will organize a conference for sharing recent studies on fundamental enzyme research, applications in the industrial technologies, and diagnoses in medicine, but also providing opportunities for communication of attendees for interdisciplinary developments in this field.
This conference is supported by ERATO Asano Active Enzyme Molecule Project and The Vitamin Society of Japan.
This conference website can be found here.

 

history

2016/04/6
"Molecular cloning of hydroxynitrile lyase from millipedes and heterologous expression in Eshcerichia coli" was awarded Hot Topics Award at The 2016 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry.
2016/04/01
Kawanishi(researcher), Shinoda(researcher), Kawahara(Technician), Zheng(Special research assistant), Tatta(administrative) joined.
2016/03/31
Oku(Technician), Zheng(Technician), Kawahara(Special research assistant), Shinnaka(research assistant), Tatta(research assistant), Yamazaki(administrative) left.
2016/3
"Heterologous production of L-lysine ε-oxidase by directed evolution using a fusion reporter method" was awarded The Excellent Paper Award Published in Bioscience, Biotechnology, & Biochemistry.
2016/02/29
Miao(researcher) left.
2015/10/09
"L-Arginine oxidase from Pseudomonas sp. TPU 7192: Characterization,gene cloning, heterologous expression, and application to L-arginine determination" was published online in Enzyme Microb.Technol.
2015/9/15
"Structural analysis of peptide legand recognition mechanism of alkaline D-peptidase from Bacillus cereus DF4-B" was published online in Sci.Rep.
2015/09/01
Zheng(Technician)、Tatta (research assistant) joined.
2015/08/25
"Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis" picked up cover picture.
2015/08/11
We paretners with JST published press release about a paper "Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis" published online in Proc.Nat.Acad.Sci.USA
2015/08/16
Murata(administrative) left.
2015/08/11
"Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis" was published online in Proc.Nat.Acad.Sci.USA
2015/08/07
"Efficient Production of Lumichrome by Microbacterium sp. Strain TPU3598" was published online in Appl.Environ.Microbiol.
2015/08/3
Miao (Researcher) received Biotrans Poster Award-Biotrans 2015 Biotrans Poster Award for excellent poster presentation and speed talk (12 awardees out of 462 posters).
2015/07/27
"Mutagenesis of Asn156 residue on surface region in S-selective HNL from Baliospermum montanum enhances catalytic efficiency and stereoselectivity" was published online in ChemBioChem.
2015/07/01
Sawamura(research assistant)、Matsunaga (research assistant) joined.
2015/04/21
"Heterologous production of L-lysine ε-oxidase by directed evolution using a fusion reporter method" was published online in Bioscience, Biotechnology, and Biochemistry.
2015/04/01
Motojima(researcher), Tani(researcher), Zhai(researcher) joined.
2015/03/31
Kameya(researcher), Okazaki(researcher), Nakano(researcher), Fuhshuku(researcher), Dadashipour(researcher), Ueda(techncian), Akae(research assistant), Itamoto(administrative) left.
2015/03/19
"Heterologous production of L-lysine ε-oxidase by directed evolution using a fusion reporter method" was accepted in Bioscience, Biotechnology, and Biochemistry,
2015/03/16
Yamazaki(administrative staff) joined.
2015/02/03
"Enhancement of stability of L-tryptophan dehydrogenase from Nostoc punctiforme ATCC29133 and its application to L-tryptophan assay" was accepted in Journal of Biotechnology and "Protein evolution analysis of S-hydroxynitrile lyase by complete sequence design utilizing the INTMSAlign software" was accepted in Scientific reports.
2015/01/13
"Complete genome sequence of an aldoxime degrader, Bacillus sp. OxB-1" was accepted in Genome Announcements
2015/01/01
Murata(administrative staff) joined.
2014/12/17
Active Enzyme Molecule 2014 was held in Toyama, thank you for coming in spite of inclement weather.
2014/12/16
We had our laboratory tour.
2014/12/10
"Chemical polymorphism of defense secretion with growth in the millipede Niponia nodulosa [Polydesmida: Cryptodesmidae]"was accepted in Jounal of Chemical Ecology.
2014/10/
"Notes on egg brooding newly recorded in two millipede species" was accepted in Japanese Journal of Entomology (New Series)
2014/10/01
Miao(researcher) joined.
2014/09/05
"Structural and functional analysis of hydroxynitrile lyase from Baliospermum montanum with crystal structure, molecular dynamics and enzyme kinetics " was accepted in BBA Proteins and Proteomics.
2014/09/02
Research director Asano received the "biocat Award – Science 2014".
2014/09/01
Izumi(researcher) joined.
2014/08/22
"Biosynthetic pathway for the cyanide-free production of phenylacetonitrile in Escherichia coli by utilizing plant cytochrome P450 79A2 and bacterial aldoxime dehydratase" was accepted in Applied and Environmental Microbiology
2014/07/05
"Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in L-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc. " was accepted in Plant Molecular Biology
2014/07/04
Miki(researcher) left.
2014/05/01
Metzner(researcher) joined.
2014/04/16
"Characterization of a pyridoxal-5'-phosphate-dependent L-lysine decarboxylase/oxidase from Burkholderia sp. AIU 395 " was accepted in Journal of Bioscience and Bioengineering
2014/04/01
Isobe(project coordinator), Fuhshuku(researcher), Shichida(techncian),Akae(research assistant), and Tauchi(staff) joined.
2014/03/31
Yasukawa(Researcher), Ichiki(Researcher), Morita(techncian), Fushimi(staff), and Yoshioka(staff)left.And Kyoto branch closed.
2014/03/
"(2-Nitroethyl)benzene: a major flower scent from the Japanese Loquat Eriobotrya japonica [Rosales: Rosaceae] " was accepted in Bioscience, Biotechnology, and Biochemistry
2014/02/20
"Binding of NAD+ and L-Threonine induces Stepwise Structural and Flexibility changes in Cupriavidus Necator L-Threonine Dehydrogenase" was accepted in The Journal of Biological Chemistry
2014/02/04
"Mutational and crystallographic analysis of L-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813: Interconversion between oxidase and monooxygenase activities" was accepted in FEBS Open Bio
2014/01/18
"Tailoring D-amino acid oxidase from pig kidney to R-stereoselective amine oxidase and its use in deracemization of α-methylbenzylamine" was accepted in Angewandte chemie International Edition
2014/01/16
"Rapid enzymatic assays for L-citrulline and L-arginine based onthe platform of pyrophosphate detection" was accepted in Enzyme and Microbial Technology
2013/11/01
"Rapid and selective enzymatic assay for L-methionine based on a pyrophosphate detection system" was accepted in Analytical Biochemistry
2013/09/30
Hummel(researcher) and Jomura(administrative coordinator) left
2013/09/26
Research director Asano received The 2013 Enzyme Engineering Award, and researcher Yasukawa received The 2013 Enzyme Engineering Poster Competition Honorable Mention.
2013/09/23
Our project organized ERATO Asano Active Enzyme Molecule Project Special Session in Enzyme Engineering XXII.
2013/08/
"X-Ray crystallographic evidence for the presence of the crysteine tryptophylquinone cofactor in L-lysine ε-oxidase from Marinomonas mediterranea" was accepted in The Journal of Biochemistry
2013/05/24
Open Laboratory
2013/05/01
Shinnaka(research assistant)joined
2013/04/01
Yamamoto(researcher),Takakura(researcher),Oku(techncian),Ueda(techncian),Kishihara(research assistant),Kawahara(special reseach assistant),Jomura(administrative coordinator)joined
2013/03/31
Tatta (research assistant),Ueda(research assistant),Akita (administrative manager),Nishino (administrative Staff)left
2013/03/18
"Selective tryptophan determination using tryptophan oxidases involved in bis-indole antibiotic biosynthesis in Analytical Biochemistry
2013/03/01
Itamoto (administrative staff) joined
2012/11/19
Hummel (Researcher) joined
2012/10/01
Ishida (Researcher) joined
2012/08/01
Ueda (research assistant),Fukagawa(research assistant)joined
2012/07/01
Ina (researcher) joined
2012/06/01
Matsuda (research manager) joined
2012/05/30
ERATO session was held in the 12th Japan-China-Korea Joint Symposium on Enzyme Engineering
2012/04/23
Yoshikawa (staff) joined
2012/04/02
Start-up meeting was held
2012/04/01
Kuwahara (group leader), Ichiki (researcher), Okazaki (researcher), Kameya (researcher), Nakano (researcher), Matsui (researcher), Miki (researcher), Yamaguchi (researcher), Morita (techncian), Ohno (research assistant), Tatta (research assistant), Akita (administrative manager), Hashi (staff) and Yoshioka (staff) joined
2012/04/01
Asano Active Enzyme Molecule Project started. Kyoto branch was opened.
2012/02/01
Fushimi (staff) joined
2012/01/01
Dadashipour (researcher) and Yasukawa (researcher) joined
2011/11/01
Nishino (administrative Staff) joined
2011/10/14
Project start meeting was held
2011/10/01
2011/10/01
JST staff was sended to Toyama Prefectual University fot start-up
2011/08/01
Prof. Asano was selected as ERATO Project Director