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活動報告

Publication list

2017/05
W. Payoungkiattikun, S. Okazaki, A. Ina, A. H-Kittikun, and Y. Asano
Characterization of an α-amino-ε-caprolactam racemase with broad substrate specificity from Citreicella sp. SE45, J. Ind. Microbiol. Biotechnol, 44 (4), 677-685, 2017
2017/05
Y. Miki, S. Okazaki, and Y. Asano
Engineering an ATP-dependent D-Ala:D-Ala ligase for synthesizing amino acid amides from amino acids, J. Ind. Microbiol. Biotechnol, 44 (4), 667-675, 2017
2017/04
Y. Kuwahara, T. Yamaguchi, Y. Ichiki, T. Tanabe, Y. Asano
Hydrogen peroxide as a new defensive compound in “benzoyl cyanide” producing polydesmid millipedes, Sci. Nat, 104 (19), 2017
2017/02
A. Nuylert, Y. Ishida, and Y. Asano
Effect of glycosylation on the biocatalytic properties of hydroxynitrile lyase from the passion fruit,Passiflora edulis - a comparison of natural and recombinant enzymes, ChemBioChem, 18 (3), 257-265, 2017
2017/02
T. Yamaguchi, Y. Kuwahara, and Y. Asano
A novel cytochrome P450, CYP3201B1, is involved in (R)-mandelonitrile biosynthesis in a cyanogenic millipede, FEBS Open Bio, 7 (3), 335-347, 2017
2017/02
N. Kawahara, K. Yasukawa, and Y. Asano
New enzymatic methods for the synthesis of primary α-aminonitriles and unnatural α-amino acids by oxidative cyanation of primary amine with D-amino acid oxidase from porcine kidney, Green Chem, 19, 418-424, 2017
2017/02
Y. Asano and D. Matsui
Development of enzymes to determine amino acids by growth-dependent molecular selection (In Japanese), Fine Chemical, 46 (2), 2017
2017/01
Y-.f. Miao, R. Metzner, and Y. Asano
Kemp elimination catalyzed by naturally occurring aldoxime dehydratases, ChemBioChem, 18 (5), 451-454, 2017
2016/12
M. Kameya and Y. Asano
Translation-dependent bioassay for amino acid quantification using auxotrophic microbes as biocatalysts of protein synthesis, Appl. Microbiol. Biotechnol, 101 (6), 2523-2531, 2017
2016/09
S. Nakano, K. Yasukawa, T. Tokiwa, T. Ishikawa, E. Ishitsubo, N. Matsuo, S. Ito, H. Tokiwa, and Y. Asano
Origin of stereoselectivity and substrate/ligand recognition in an FAD-dependent R-selective amine oxidase, J. Phys. Chem. B, 120 (41), 10736-10743, 2016
2016/07
T. Yamaguchi, K. Noge, and Y. Asano
Cytochrome P450 CYP71AT96 catalyses the final step of herbivore-induced phenylacetonitrile biosynthesis in the giant knotweed, Fallopia sachalinensis, Plant Mol. Biol, 91 (3), 1-11, 2016
2016/06
Y. Ishida, Y. Kuwahara, M. Dadashipour, A. Ina, T. Yamaguchi, M. Morita, Y. Ichiki, and Y. Asano
A sacrificial millipede altruistically protects its swarm using a drone blood enzyme, mandelonitrile oxidase, Scientific Reports, 6, 26998, 2016
2016/05
T. Yamaguchi, and Y. Asano
Draft genome sequence of an aldoxime degrader, Rhodococcus sp. strain YH3-3, Genome announcements, 4(3), e00406-16, 2016
2016/05
Y. Asano, S. Okazaki, and K. Yasukawa
Recent developments in amino peptidases, oxidases and racemases,In Biocatalysis-Green Technology, Ed. Ramesh Patel, Chapter 19, pp489-502, 2016
2016/04
Y. Asano and N. Kawahara
A new S-hydroxynitrile lyase from Baliospermum montanum Its structure, molecular dynamics simulation, and improvement by protein engineering, Industrial Biotechnology, 12 (2), 91-97, 2016. Cover picture
2016/02
T. Yamaguchi, K. Noge, and Y. Asano
Cytochrome P450 CYP71AT96 catalyses the final step of herbivore-induced phenylacetonitrile biosynthesis in the giant knotweed, Fallopia sachalinensis, Plant Mol. Biol., 91 (3), 229-39, 2016.Cover picture
2016/01
Y. Asano
Enchanted by Enzyme Discovery -Developing biological power-(In Japanese), Modern Chemistry (Gendai Kagaku), 539 (2), 48-53,2016
2016/01
Daisuke Matsui, Anna Terai, and Yasuhisa Asano
L-Arginine oxidase from Pseudomonas sp. TPU 7192: Characterization, gene cloning, heterologous expression, and application to L-arginine determination, Enzyme Microb.Technol., 82:151-157,2015
2015/11
Kazunori Yamamoto, and Yasuhisa Asano
Efficient Production of Lumichrome byMicrobacterium sp. Strain TPU3598, Appl.Environ.Microbiol., 81:7360-7367,2015
2015/09
Shogo Nakano, Seiji Okazaki, Erika Ishitsubo, Nobuhiro Kawahara, Hidenobu Komeda, Hiroaki Tokiwa, Yasuhisa Asano
Structural analysis of the peptide ligand recognition mechanism of alkaline D-peptidase from Bacillus cereus DF4-B, Sci.rep., 5:13836, 2015
2015/08
Mohammad Dadashipour, Yuko Ishida, Kazunori Yamamotoa, and Yasuhisa Asano
Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede,Chamberlinius hualienensis, Proc.Nat.Acad.Sci.USA, 112(34):10605-10610, 2015
2015/07
Nobuhiro Kawahara,and Yasuhisa Asano
Mutagenesis of Asn156 residue on surface region inS-selective HNL fromBaliospermum montanum enhances catalytic efficiency and stereoselectivity, ChemBioChem, 16:1891-1895,2015
2015/04
Daisuke Matsui, Yasuhisa Asano
Heterologous production of L-lysine ε-oxidase by directed evolution using a fusion reporter method, Bioscience, Biotechnology, and Biochemistry, 79(9):1473-1480, 2015
2015/02
Shogo Nakano, Yasuhisa Asano
Protein evolution analysis ofS-hydroxynitrile lyase by complete sequence design utilizing the INTMSAlign software, Scientific reports, 5(8193), DOI: 10.1038/srep08193
2014/10
Shin-ichi Kudo, Yasumasa Kuwahara, Zoltán Korsós, Yayoi Ichiki, Masashi Morita, Yasuhisa Asano, Yasuyuki Nakamura, Tsutomu Tanabe
Notes on egg brooding newly recorded in two millipede species, 昆蟲(ニューシリーズ), 17: 135–137, 2014
2015/1
Yasumasa Kuwahara, Yayoi Ichiki, Masashi Morita, Tsutomu Tanabe, Yasuhisa Asano
Chemical polymorphism of defense secretion with growth in the millipedeNiponia nodulosa [Polydesmida: Cryptodesmidae] , Journal of Chemical Ecology, 41, 15-21, 2015
2014/12
Shogo Nakano, Mohammad Dadashipour, Yasuhisa Asano
Structural and functional analysis of hydroxynitrile lyase fromBaliospermum montanum with crystal structure, molecular dynamics and enzyme kinetics , BBA Proteins and Proteomics, 1844(12): 2059-2067, 2014
2014/11
Yuta Miki, Yasuhisa Asano
Biosynthetic pathway for the cyanide-free production of phenylacetonitrile inEscherichia coli by utilizing plant cytochrome P450 79A2 and bacterial aldoxime dehydratase, Applied and Environmental Microbiology, 80(21): 6828-36, 2014
2014/11
Richard Metzner, Seiji Okazaki, Yasuhisa Asano, Harald Gröger
Cyanide-free enantioselective synthesis of nitriles: synthetic proof of a biocatalytic concept and mechanistic insights ChemCatChem, 11, 3105–3109, 2014
2014/09
Takuya Yamaguchi, Kazunori Yamamoto, Yasuhisa Asano
Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in L-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot,Prunus mume Sieb. et Zucc. Plant Molecular Biology, 86(1-2): 215-23, 2014
2014/11
Asami Sugawara, Daisuke Matsui, Narumi Takahashi, Miwa Yamada, Yasuhisa Asano, Kimiyasu Isobe
Characterization of a pyridoxal-5'-phosphate-dependent L-lysine decarboxylase/oxidase fromBurkholderia sp. AIU 395 Journal of Bioscience and Bioengineering, 118(5): 496-501, 2014
2014/08
Yasumasa Kuwahara, Yayoi Ichiki, Masashi Morita, Yasuhisa Asano
(2-Nitroethyl)benzene: a major flower scent from the Japanese LoquatEriobotrya japonica [Rosales: Rosaceae] Bioscience, Biotechnology, and Biochemistry, 78(8): 1320-1323, 2014
2014/02
Daisuke Matsui, Do-Hyun Im, Asami Sugawara, Yasuhisa Fukuta, Shinya Fushinobu, Kimiyasu Isobe, Yasuhisa Asano
Mutational and crystallographic analysis of L-amino acid oxidase/monooxygenase fromPseudomonas sp. AIU 813: Interconversion between oxidase and monooxygenase activities. FEBS Open Bio 4: 220–228, 2014
2014/04
Shogo Nakano, Seiji Okazaki, Hiroaki Tokiwa, Yasuhisa Asano
Binding of NAD+ and L-Threonine induces Stepwise Structural and Flexibility changes inCupriavidus Necator L-Threonine Dehydrogenase The Journal of Biological Chemistry 289(15):10445-54, 2014
2014/04
Kazuyuki Yasukawa, Shogo Nakano, Yasuhisa Asano
Tailoring D-amino acid oxidase from pig kidney toR-stereoselective amine oxidase and its use in deracemization of α-methylbenzylamine. Angewandte chemie International Edition 53(17):4428-31, 2014
2014/04
Masafumi Kameya, Yasuhisa Asano
Rapid enzymatic assays for L-citrulline and L-arginine based onthe platform of pyrophosphate detection. Enzyme and Microbial Technology 57: 36-41, 2014
Journal, Author manuscript
2014/02
Masafumi Kameya, Mariko Himi, Yasuhisa Asano
Rapid and selective enzymatic assay for L-methionine based on a pyrophosphate detection system. Analytical Biochemistry 447: 33-38, 2014
Journal, Author manuscript
2013/08
Seiji Okazaki, Shogo Nakano, Daisuke Matsui, Shusaku Akaji, Kenji Inagaki, Yasuhisa Asano
X-Ray crystallographic evidence for the presence of the cysteine tryptophylquinone cofactor in L-lysine ε-oxidase fromMarinomonas mediterranea. The Journal of Biochemistry 154(3):233-236, 2013
2013/03
Masafumi Kameya, Hiroyasu Onaka, Yasuhisa Asano
Selective tryptophan determination by using tryptophan oxidases involved in bis-indole antibiotic biosynthesis. Analytical Biochemistry 438(2): 124-132, 2013
Journal, Author manuscript

Oral or Poster Presentation list

2016/11
○Aem nuylert, C.Khanongnuch, Yasuhisa Asano   The Occurrence of Hydroxynitrile lyase in Two Pssion Fruit leaves, Passiflora edulis Sims and Passiflora edulis forma flavicarpa and Their Application in Asymmetric Synthesis of (R)-mandelonitrile , The 28th Annual Meerting of the Thai Society for Biotechnology and International Conference
2016/11
○Yasuhisa Asano   Explotion and moleculer characterization of hydroxynitrile lyase, an industrial enzyme from nature,The 28th Annual Meerting of the Thai Society for Biotechnology and International Conference
2016/11
○Yasuhisa Asano   Discovery of R-sterepselective amine oxidase by protein engineering of porcine kidney D-amino acid oxidase, and its use for chiral (S)-amine systhesis and oxidative cyanation reaction,The 14th China-Japan-Korea Joint Symposium on Enzyme Engineering
2016/9
○Aem Nuylert, Takuya Yamaguchi, Yuko Ishida, Yasumasa Kuwahara, Yasuhisa Asano   Molecular cloning of the full-length cDNA of ynamics of hydroxynitrile lyase from a millipede, Parafontaria laminata and its expression in Escherchia coli, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Yasushi Tani, Yuko Ishida, Yasuhisa Asano   Overexpression of Vargula luciferase using co-expression system with Vargula hilgendrifii protein disulfide isomerase gene in Pichia pastoris, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Zhenyu Zhai, Yasuhisa Asano   Overexpression of Chemberlinius hualienesis hydroxynitrile lyase in Pichia pastoris by co-expression of protein disulfide isomerase, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Takuya Yamaguchi, Koji Noge, Yasuhisa Asano   Identification and characterization of CYP71AT96 catalyzing the final step of herbivore-induced phenylacetonitrile biosynthesis in giant knodweed, Fallopia sachalinensis, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Yuko Ishida, Yasumasa Kuwahara, Mohammad Dadashipour, Atsutoshi Ina, Takuya Yamaguchi, Masashi Morita, Yayoi Ichiki, Yasuhisa Asano   Mandelonitrile oxidase from the millipede's blood as a defensive enzyme stoichiometrically synthesizing bezoyl cyanide and hydrogen peroxide from (R)-mandelonitrile, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Richard Metzner, Yasuhisa Asano   Flavin-dependent alteration of bacterial aryl-alkyl nitrile degradation towards ketones and carbinols, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Daisuke Matsui, Yasuhisa Asano   Exploration of the soluble variants of aggregated enzymes in heterologous expression system by amino acid substitution, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Seiji Okazaki, Shogo Nakano, Daisuke Matsui, Shusaku Akaji, Kenji Inagaki, Yasuhisa Asano   Structural study of a cysteine tryptophylquinone-dependent oxidase, L-lysine Ɛ-oxidase from Marinomonas mediterranea, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Atsutoshi Izumi, Zhenyu Zhai, Takuya Yamaguchi, Sayaka Shichida, Yasuhisa Asano   Function and crystallographic analysis of hydroxynitrile lyase from a millipede Chemberlinius hualienensisin in lipocalin family, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Fumihiro Motojima, Aem Nuylert, Yasuhisa Asano   Crystal structureb and functional analysis of hydroxynitrile lyase from passion fruit, Passiflora edulis, The Fifth International Conference on Cofactors Active Enzyme Molecule 201 (Poster)6
2016/9
○Atsutoshi Ina, Yasuhisa Asano   Apppication of ion mobility mass spectrometry to glycan analysis, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Nobuhiro Kawahara, Kazuyuki Yasukawa, Yasuhisa Asano   Enzymatic synthesis of α-aminonitrile and α-methylamino acid with D-amino acid oxudase from porcine kidney be oxidative cyanation of primary amine, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Daijun Zheng, Zhenyu Zhei, Takuya Yamaguchi, Nuylert Aem, Yasuhisa Asano   Asymmetric Henry reaction catalyzed by novel R-hydroxynitrile lyase from millipedes, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Yasumasa Kuwahara, Tsutomu Tanabe, Yasuhisa Asano   Distribution of HNL and MOX among cyanogenic polydesmid millipedes [Polydesmida: Diplopoda], The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Sayaka Shichida, Ryoko Ueda, Daisuke Matsui, Shinsuke Miki, Tetsuro Nishii, Akihiro Ishii, Kimiyasu Isobe, Yasuhisa Asano   Characterization of two alcohol dehysrogenases from Ogataea polymorpha NBRC 0799 for production of (S)-1,1,1-trifluoro-2-propanol, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Kazunori Yamamoto, Atsutoshi Ina, Yuko Oku, Atsushi Izumi, Masaharu Doya, Syuji Ebata, Yasuhisa Asano   Asymmetric synthesis of (S)-muscone with a novel econe reductase from Sporidiobolus salmonicolor CBS 490, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016 (Poster)
2016/9
○Daisuke Matsui   Exploration of new methods to assign aggregation hot-spots focused on secondary structure and amino acid hydrophobicity, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016
2016/9
○Fumihiro Motojima, Shogo Nakano   Structure and bioinfomatics analysis of industrially-promising enzymes for protein engineering, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016
2016/9
○Kazunori Yamamoto   Development of new enzymatic methods for efficient production of useful chemicals, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016
2016/9
○Takuya Yamaguchi, Mohammad Dadashipour   Exploration of novel biocatalysts from plants and arthropods, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016
2016/9
○Yasuhisa Asano   Introduction to the ERATO session, The Fifth International Conference on Cofactors Active Enzyme Molecule 2016
2016/7
○Takuya Yamaguchi, Yasuhisa Asano   A cytochrome P450 involved in cyanogenesis of a Polydesmid millipede, Chamberlinius hualoenensis Wang, The 13th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
2016/7
○Yasuhisa Asano   Evolution of porcine kidney D-amino acid oxidase into R-stereoselective amine oxidase and its application for chiral (S)-amine synthesis, Gordon Research Conference (Poster)
2016/7
○Fumihiro Motojima, Yasuhisa Asano   Crystal structure and functional analysis of hydroxynitorile lyase from passion fruits, Passiflora edulis, 17th European Congress on Biotechnology (Poster)
2016/7
○Aem Nuylert, Yuko Ishida, Yasuhisa Asano   Contribution of N-glycosylation site to the enzyme stability of hydroxynitrile lyase from Passiflora edulis in the differential expression system, 17th European Congress on Biotechnology (Poster)
2016/6
○Yasuhisa Asano   Cyanide-free nitrile synthesis and “metabolic tuning” of molecular parts, 11th Metabolic Engineering Conference
2016/6
○Yasuhisa Asano   Development of Enzymatic Processes for Industrial Applications-from enzyme discovery side-, National University of Singapore
2016/5
○Yasuhisa Asano   Enzymatic Processes for Industrial Applications, Institute of Chemical Engineering Science (ICES)
2016/5
○Yasuhisa Asano   Enzymatic Processes for Industrial Applications, Bioenergy and Biorefinery Conference - Southeast Asia 2016
2016/5
○Yasuhisa Asano   Development of Enzymes for Industrial Uses, Center for Bioindustrial Technology Agency for the Assessment and Application of Technology
2016/4
○Yasuhisa Asano   Creation of R-stereoselective amine oxidase from porcine kidney D-amino acid oxidase for chiral (S)-amine synthesis, 2016 Korean Society for Biotechnology and Bioengineering
2015/12
○Yasuhisa Asano   Enzymes from microbial, plant and animal aldoxime-nitrile pathways and their applications, The International Chemical Congress of Pacific Basin Societies
2015/11
○Yasuhisa Asano   Protein engineering of porcine kidney D-amino acid oxidase to create R-stereoselective amine oxidase for chiral (S)-amine synthesis, Asian Congress on Biotechnology 2015
2015/09
○Daisuke Matsui, Do-Hyun Im, Asami Sugawara, Yasuhisa Fukuta, Shinya Fushinobu, Kimiyasu Isobe and Yasuhisa Asano   L-Amino acid oxidase/monooxygenase from Pseudomonas sp. AIU813: Characterization and interconversion of the bifunctional activities by mutagenesis, 18th Japanese-German Workshop Enzyme Technology, Kyoto
2015/09
○Yasuhisa Asano   Invasive millipede harbors speedy R-hydroxynitrile lyase , 18th Japanese-German Workshop Enzyme Technology, Kyoto
2015/09
○Yasuhisa Asano   Microbial, plant and animal aldoxime-nitrile pathways –Discovery of new enzymes, their comparative studies and applications , Enzyme Engineering XXIII, Florida, USA
2015/09
○Metzner Richard, Yasuhisa Asano   A biomimetic approach to coelenterazine-type luciferins, Enzyme Engineering XXIII, Florida, USA
2015/08
Kazuyuki Yasukawa, ○Atsushi Ono, Yasuhisa Asano    Screening of mutant D-amino acid oxidase from pig kidney and functional analysis , 11th D-amino acid research society gakujutsu kouenkai, Nagaoka
2015/08
○Nobuhiro Kawahara, Kazuyuki Yasukawa, Yasuhisa Asano    Addition of cyanide catalyzed by engineered D-amino acid oxidase from pig kidney , 11th D-amino acid research society gakujutsu kouenkai, Nagaoka
2015/07
○Yasuhisa Asano    Newly Discovered Enzymes and Their Applications, Biotrans 2015, Vienna, Austria
2015/07
○Kazunori Yamamoto, Yasuhisa Asano    Efficient Production of Lumichrome byMicrobacterium sp. TPU3598, Biotrans 2015, Vienna, Austria
2015/07
○Yufeng Miao, Yasuhisa Asano    Kemp Elimination Catalyzed by Naturally Existing Aldoxime Dehydratases, Biotrans 2015, Vienna, Austria
2015/06
○Yasuhisa Asano   In-silico screening for amino acid amide racemase and its use in dynamic kinetic resolution of α-aminonitriles, 2015 BEST Conference International Symposium on Biotechnology and Bioengineering, Taipei City, Taiwan
2015/03
○Yasumasa Kuwahara, Tsutomu Tanabe, Yasuhisa Asano    Identification of 2-alkanones from Jurid millipedesAnaulaciulus sp., Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Nobuhiro Kawahara, Yasuhisa Asano    Improvement of stereoselectivity and catalytic efficiency of hydroxynitrile lyase fromBaliosperumum montanum by mutagenesis, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Yuko Ishida, Atsutoshi Ina, Yasuhisa Asano    Purification, characterization, and tissue-specific localization of mandelonitrile oxidase from the invasive millipede,Chamberlinius hualienensis, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Takuya Yamaguchi, Yuta Miki, Koji Noge, Yasuhisa Asano    Identification of methyl jasmonate induced phenylacetonitrile biosynthesizing cytochrome P450 in the giant knotweed, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Kimiyasu Isobe, Asami Sugawara, Daisuke Matsui, Miwa Yamada, Yasuhisa Asano,    Characterization of three enzymes with 4-aminobutylamide oxidase activity fromAspergillus carbonarius AIU 205, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Ryoko Ueda, Daisuke Matsui, Kimiyasu Isobe, Tetsurou Nishii, Akihiro Ishii, Yasuhisa Asano    Purification and characterization of alcohol dehydrogenase fromOgataea polymorpha NBRC 0799, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Kazunori Yamamoto, Yasuhisa Asano    Efficient production of lumichrome byMicrobacterium sp. TPU3598, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Daisuke Matsui, Yasuhisa Asano    Heterologous expression of inclusion body as active enzymes by random mutagenesis, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Aem Nuylert, Yuko Ishida, Yasuhisa Asano    Characterization of wild-type and recombinant hydroxynitrile lyase fromPassiflora edulis inEscherichia coli andPichia pastoris - effect of glycosylation on the enzyme stability, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2015/03
○Shingo Nagamori, Kazunori Yamamoto, Atsutoshi Ina, Masaharu Doya, Shuta Kihara, Yasuhisa Asano    Purification and characterization of old yellow enzyme fromYarrowia lipolytica TPU1602, Japan Society for Bioscience, Biotechnology, and Agrochemistry 2015, Okayama
2014/12
○Yasuhisa Asano    Introduction to the ERATO session, Active Enzyme Molecule 2014, Toyama
2014/12
○Kazuyuki Yasukawa, Shogo Nakano, Yasuhisa Asano   Newly evolvedR-stereoselective amine oxidase applicable to a chiral (S)-amine synthesis, Active Enzyme Molecule 2014, Toyama
2014/12
○Yuko Ishida, Mohammad Dadashipour, Takuya Yamaguchi, Yuta Miki, Kazunori Yamamoto, Atsutoshi Ina, Yayoi Ichiki, Yasumasa Kuwahara, Yasuhisa Asano    Plants and animals are valuable bioresources for exploring novel enzymes, Active Enzyme Molecule 2014, Toyama
2014/12
○Mohammad Dadashipour, Yuko Ishida, Kazunori Yamamoto, Yasuhisa Asano   Hydroxynitrile lyase from a millipede as a valuable tool for white biotechnology: purification, characterization and recombinant expression, Active Enzyme Molecule 2014, Toyama
2014/12
○Masafumi Kameya, Daisuke Matsui, Yasuhisa Asano    New enzymatic and microbial methods for selective assay of amino acid, Active Enzyme Molecule 2014, Toyama
2014/12
○Daisuke Matsui, Yasuhisa Asano    Soluble expression inEscherichia coli of heterologous enzymes by mutagenesis, Active Enzyme Molecule 2014, Toyama
2014/12
○Shogo Nakano, Seiji Okazaki, Kazuyuki Yasukawa, Mohanmad Dadashipour, Hiroaki Tokiwa, Yasuhisa Asano    Endeavors to bridge a gap between wet and dry science in enzyme engineering, Active Enzyme Molecule 2014, Toyama
2014/12
○Kimiyasu Isobe, Asami Sugawara, Daisuke Matsui, Miwa Yamada, Yasuhisa Asano   Characterization of L-lysine decarboxylase/oxidase fromBurkholderia sp. AIU 395 and its application to assay of L-lysine, Active Enzyme Molecule 2014, Toyama (Poster)
2014/12
○Masafumi Kameya, Yasuhisa Asano   Application of aminoacyl-tRNA synthetase for selective determination of amino acids, Active Enzyme Molecule 2014, Toyama (Poster)
2014/12
○Daisuke Matsui, Yasuhisa Asano   Heterologous expression of L-lysine ε-oxidase by directed evolution using a fusion reporter method, Active Enzyme Molecule 2014, Toyama (Poster)
2014/12
○Richard Metzner, Seiji Okazaki, Yasuhisa Asano, Harald Groeger   Enantioselective aldoxime dehydratases – Synthetic proof andin silico studies, Active Enzyme Molecule 2014, Toyama (Poster)
2014/12
○Shogo Nakano, Yasuhisa Asano   Application of INTMSAlign to design functional sequences ofS-selective hydroxynitrile lyase, Active Enzyme Molecule 2014, Toyama (Poster)
2014/12
○Seiji Okazaki, Shogo Nakano, Nobuhiro Kawahara, Hidenobu Komeda, Yasuhisa Asano   Structural study of an alkaline D-stereospecific endopeptidase fromBacillus cereus DF4-B, Active Enzyme Molecule 2014, Toyama (Poster)
2014/12
○Takuya Yamaguchi, Yasuhisa Asano   Biocatalytic synthesis of (R)-mandelic acid from L-phenylalanine using a combination of plant cytochrome P450s and bacterial nitrilase, Active Enzyme Molecule 2014, Toyama (Poster)
2014/12
○Kazunori Yamamoto, Yasuhisa Asano   Efficient production of lumichrome byMicrobacterium sp. TPU3598, Active Enzyme Molecule 2014, Toyama (Poster)
2014/12
○Nobuhiro Kawahara, Yasuhisa Asano   Improvement of the stereoselectivity and catalytic efficiency by mutagenesis in hydroxynitrile lyase fromBaliospermum montanum, Active Enzyme Molecule 2014, Toyama (Poster)
2014/11
○Yasuhisa Asano   Structure and application of amino acid metabolizing enzymes, 13th China-Japan-Korea Joint Symposium on Enzyme Engineering, Jeju Island, Korea
2014/11
○Mohammad Dadashipour, Shogo Nakano, Yasuhisa Asano   Structural and functional analysis of hydroxynitrile lyase fromBaliospermum montanum with crystal structure, molecular dynamics and enzyme kinetics, Japan-Italy Symposium 2014, Nara
2014/11
○Richard Metzner, Seiji Okazaki, Harald Gröger, Yasuhisa Asano   Synergetic analysis of enantioselective aldoxime dehydratases by combination ofin vitro andin silico methods, Japan-Italy Symposium 2014, Nara
2014/11
○Yasuhisa Asano   In silico Screening, Structure and Application of Enzymes Related to Amino Acid Metabolism, Japan-Italy Symposium 2014, Nara
2014/10
○Yasuhisa Asano   Novel enzymes for organics synthesis and diagnostics uses, 4th International Conference on Novel Enzymes, Ghent, Belgium
2014/09
Shogo Nakano, ○Seiji Okazaki, Nobuhiro Kawahara, Hidenobu Komeda, Yasuhisa Asano   Crystal structure of an alkaline D-stereospecific endopeptidase fromBacillus cereus, The 2nd International Conference of D-Amino Acid Research, Utsunomiya
2014/08
○Takuya Yamaguchi, Kazunori Yamamoto, Yasuhisa Asano   Identification and characterization of CYP79D16 and CYP71AN24 involved in the mandelonitrile biosynthesis from L-phenylalanine in the Japanese apricot, 7th international congress on biocatalysis, Humburg, Germany (Poster)
2014/08
○Yasuhisa Asano, Kazuyuki Yasukawa, Shogo Nakano   Creation of R-stereoselective amine oxidase from porcine kidney D-amino acid oxidase and its use in deracemization of a-methylbenzylamine, The Fourth International Conference on Cofactors, Parma, Italy
2014/08
○Seiji Okazaki, Shogo Nakano, Daisuke Matsui, Shusaku Akaji, Kenji Inagaki, Yasuhisa Asano   A novel structure of a cysteine tryptophylquinone-dependent oxidase, L-lysine ε-oxidase fromMarinomonas mediterranea, The Fourth International Conference on Cofactors, Parma, Italy
2014/08
○Yaushisa Asano   Utilization of L-lysine ε-oxidase and engineered D-amino acid oxidase for L-lysine determination and chiral amine synthesis, respectiverly, The 3rd Toyama-Basel Joint Symposium on Pharmaceutical Reserchand Drug Development, Toyama
2014/07
○Daisuke Matsui, Do-Hyun Im, Shinya Fushinobu, Kimiyasu Isobe, Yasuhisa Asano   L-Amino acid oxidase/monooxygenase fromPseudomonas sp. AIU 813: characterization and mutational analysis, IUBMB Symposium : 18th International Symposium on Flavins and Flavoproteins, Phechburi, Thailand (Poster)
2014/07
○Yasuhisa Asano, Kazuyuki Yasukawa, Shogo Nakano   Tailoring D-amino acid oxidase from pig kidney toR-stereoselective amine oxidase and its use in deracemization of a-methylbenzylamine, Gordon Research Conference 2014 Biocatalysis, Smithfield, RI ,USA (Poster)
2014/07
○Nobuhiro Kawahara, Yasuhisa Asano  Improvement of hydroxynitrile lyase fromBaliosperumum montanum by mutagenesis, Gordon Research Conference 2014 Biocatalysis, Smithfield, RI, USA (Poster)
2014/03
○Kazuyuki Yasukawa, Yasuhisa Asano  Amino acid oxidase-catalyzed Strecker reaction, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Yuta Miki, SEiji Okazaki, Yasuhisa Asano  Screening of phenylacetaldoxime-converting cytochrome P450s inArabidopsis thaliana, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Mohammad Dadashipour, Yuko Ishida, Yasuhisa Asano  A new hydroxynitrile lyase: purification, characterization and recombinant expression, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Seiji Okazaki, Shogo Nakano, Daisuke Matsui, Shusaku Akaji, Kenji Inagaki, Yasuhisa Asano   Crystallographical evidence for the presence of the cysteine tryptophylquinone cofactor in L-Lysine ε-oxidase fromMarinomonas mediterranea, Annual Meeting of Japan Society for iosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Masafumi Kameya, Yasuhisa Asano  Application of aminoacyl-tRNA synthetases to amino acid determination, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Daisuke Matsui, Yasuhisa Asano  Heterologous expression of L-lysine ε-oxidase by directed evolution; Selection of the expressible variant by fusion with an antibiotic marker, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Takuya Yamaguchi, Yasuhisa Asano  Identification and characterization of P450s fromPrunus mume Sieb. Et Zucc in the biosynthesis of amygdalin, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Ina Atsutoshi, Yasuhisa Asano  Functional expression of dehydrogenase fromSchizosaccharomyces pombe inEscherichia coli, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Yasumasa Kuwahara,Yayoi Ichiki,Masashi Morita, Yasuhisa Asano  Chemical polymorphism of defense secretions along with growth in the millipedeNiponia nodulosa [Polydesmida: Cryptodesmidae], Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Yayoi Ichiki, Masashi Morita, Yuko Ishida, Mohammad Dadashipour, Takuya Yamaguchi, Yasumasa Kuwahara, Yasuhisa Asano  Purification and characterization of mandelonitrile oxidase from the millipedeChamberlinius hualienensis, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Nobuhiro Kawahara, Yasuhisa Asano  Screening and analysis of the soluble mutants at hydroxynitrile lyase in E.coli expression system, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Asami Sugawara, Daisuke Matsui, Miwa Yamada, Yasuhisa Asano, Kimiyasu Isobe   Characterization of an enzyme with decarboxylase and oxidase activities fromBurkholderia sp.AIU 395, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
Seiji Okazaki, Shogo Nakano, ○Hiroaki Tokiwa, Yasuhisa Asano  Multiscale Simulations of Plant Hydroxynitrile Lyase using the First-Principles Calculations & MD Simulations, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Chiaki Yoshikawa, Masafumi Kameya, Yasuhisa Asano  Identification of aromatic compounds in plants and characterization of alcohol acyltransferase, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Shunsuke Watanabe, Ken-ichi Fuhshuku, Tetsuro Nishii, Akihiro Ishii, Yasuhisa Asano  Purification, gene cloning and expression of enantioselective amidase acting on α-(trifluoromethyl)lactamide, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/03
○Kana Hounoki, Kazuyuki Yasukawa, Yasuhisa Asano  Screening of thermostable nitrile hydratase, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2014, Tokyo
2014/01
○Yasuhisa Asano, Mohammad Dadashipour  Mechanism of soluble expression of a mutant plant enzyme hydroxynitrile lyase, Gordon Research Conference 2014 Protein Folding Dynamics,Galveston, TX, USA (Poster)
2013/09
○Yasuhisa Asano  Development of enzymes for industrial and diagnostic uses, EnzymeEngineeringXXII,Toyama
2013/09
○Seiji Okazaki, Yasuhisa Asano  Crystallographic evidence for the presence of the cysteine tryptophylquinone cofactor in L-Lysine ε-oxidase fromMarinomonas mediterranea, EnzymeEngineeringXXII, Toyama
2013/09
○Yuta Miki, Yasuhisa Asano  Microbial production of phenylacetonitrile utilizing enzymes from " the aldoxime-nitrile pathway ", EnzymeEngineeringXXII, Toyama
2013/09
○Seiji Okazaki,Yasuhisa Asano  Switching open and closed conformation of L-Threonine Dehydrogenase fromCupriavidus necator, EnzymeEngineeringXXII, Toyama (Poster)
2013/09
○Kazuyuki Yasukawa, Yasuhisa Asano   Production of (S)-methylbenzylamine by deracemization of its racemic mixture using newly evolved amine oxidase from porcine kidney D-amino acid oxidase, EnzymeEngineeringXXII,Toyama (Poster)
2013/09
○Shogo Nakano, Yasuhisa Asano  Development of multiple sequence alignment method to support a design of site-directed mutants: INTMSAlign, EnzymeEngineeringXXII,Toyama (Poster)
2013/09
○Daisuke Matsui, Yasuhisa Asano  Enhancement of stability and catalytic activity of L-tryptophan dehydrogenase by directed evolution for L-tryptophan determination, EnzymeEngineeringXXII,Toyama (Poster)
2013/09
○Masafumi Kameya,Yasuhisa Asano  Enzymatic determination of amino acids by coupling aminoacyl-tRNA synthetase and pyrophosphate detection system, EnzymeEngineeringXXII,Toyama (Poster)
2013/07
○Yasuhisa Asano  Recent progress in ERATO Asano Active Enzyme Molecule project:1. Enzymatic nitrile synthesis andin-silico screening for amino acid amide racemase 2. L-Amino acid oxidase/oxygenase, and tryptophan dehydrogenase - Evolution of selective enzymes for microdetermination of amino acids, 17th German-Japanese Workshop on Enzyme Technology,Hamburg, Germany
2013/07
○Daisuke Matsui, Kimiyasu Isobe, Yasuhisa Asano  Application of an L-amino acid oxidase/oxygenase to amino acid assay:Interconversion between oxidase and oxygenase activities, BioTrans2013, Manchester, UK (Poster)
2013/07
○Yuta Miki, Yasuhisa Asano  Potential white-biotechnology for production of nitrile compounds utilizing ‘the aldoxime-nitrile pathway’, BioTrans2013,Manchester, UK (Poster)
2013/06
○Masafumi Kameya, Yasuhisa Asano  Selective quantification of amino acids by enzymatic assays, Application for Enzyme Technologies 2013,San Francisco, USA (Poster)
2013/06
○Yasuhisa Asano  Biopolyamid Platform, 10th World Congress on Industiral Biotechnology,Montreal,Canada
2013/05
Shogo Nakano, Seiji Okazaki, Yasuhisa Asano  Open and closed structures of GalE-like L-Threonine dehydrogenase fromCupriavidus necator apperared from X-rey crystal structures and molecular dynamics simulation, ISDSB 2013,Nagoya (Poster)
2013/05
Seiji Okazaki, Shogo Nakano, Yasuhisa Asano  Molecular dynamics of Manihot esculenta hydroxynitrile lyase, ISDSB 2013,Nagoya (Poster)
2013/03
○Dohyun IM, Daisuke Matsui, Kimiyasu Isobe, Yasuhisa Asano, Shinya Fushinobu  Structure based study of a novel bifunctional flavoenzyme L-amino acid monooxygenase/oxidase fromPseudomonas sp. AIU 813, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2013, Sendai
2013/03
○Anna Terai,Daisuke Matsui, Yasuhisa Asano  Screening of highly substrate-specific L-arginine oxidase for L-amino acid determination, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2013, Sendai
2013/03
○Kazushi Yasukawa, Yasuhisa Asano  Deracemization of (RS)-alpha-methylbenzylamine using engineered D-amino acid oxidase from porcine kidney, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2013, Sendai
2013/03
○Daisuke Matsui, Yasuhisa Asano  Screening of stable L-tryptophan dehydrogenase mutant by use of E. coli tryptophan auxotroph, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2013, Sendai
2013/03
○Yuta Miki, Yasuhisa Asano  Production of phenylacetonitrile by recombinant Escherichia coli, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2013, Sendai
2013/03
○Yasumasa Kuwahara, Yayoi Ichiki,Masashi Morita,Yasuhisa Asano  Natural Product: Enzyme Reaction during Hexane Extraction, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2013, Sendai
2013/03
○Shogo Nakano, Yasuhisa Asano  Development of derivative program of ClustalW, INTMSAlign, and prediction of hydrogen bond network of enzymes, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2013, Sendai
2013/03
○Masafumi Kameya, Yasuhisa Asano  Proposal of the "translation-dependent" bioassay, a novel concept of amino acid assays, Annual Meeting of Japan Society for Biosience,Biotechinology,and Agrochemistry 2013, Sendai
2012/10
○Shogo Nakano, Yasuhisa Asano  Derivative program of ClustalW can predict candidate residues which improve protein solubility, The Society of Biotechinology, Japan, 64th Annual Meeting(2012), Kobe
2012/10
○Masafumi Kameya, Yasuhisa Asano  Development of novel assays for amino acids coupling to pyrophosphate-detection as a platform, The Society of Biotechinology,Japan,64th Annual Meeting(2012), Kobe
2012/07
○Masafumi Kameya and Yasuhisa Asano  Selective and enzymatic quantification of amino acids based on pyrophosphate detection, EMBO2012, Groningen, Netherlands (Poster)