中内幹細胞制御プロジェクト

論文発表成果

2012

• Homeostasis of hematopoietic stem cells regulated by myeloproliferative disease associated gene product Lnk/Sh2b3 via Bcl-xL. Nao Suzuki, Satoshi Yamazaki, Hideo Ema, Tomoyuki Yamaguchi, Hiromitsu Nakauchi, and Satoshi Takaki. Experimental Hematology, Vol. 40, Issue 2, Februaly 2012, Pages 166-174.
• Hollow fiber vitrification: a novel method for vitrifying multiple embryos in a single device. Matsunari H, Maehara M, Nakano K, Ikezawa Y, Hagiwara Y, Sasayama N, Shirasu A, Ohta H, Takahashi M, Nagashima H. Journal of Reproduction Development. 2012;58(5):599-608.
• Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming. Tomoyuki Yamaguchi, Sanae Hamanaka, Akihide Kamiya, Motohito Okabe, Mami Kawarai, Yukiko Wakiyama, Ayumi Umino, Tomonari Hayama, Hideyuki Sato, Youn-Su Lee, Hideki Masaki, Toshihiro Kobayashi, Satoshi Yamazaki, and Hiromitsu Nakauchi. PloS One July 2012, Vol.7 (7),
• Identification of Rat Rosa26 Locus Enables Generation of Knock-in Rat Lines Ubiquitously ExpressingtdTomato. Toshihiro Kobayashi, Megumi Kato-Itoh, Tomoyuki Yamaguchi, Chihiro Tamura, Makoto Sanbo, Masumi Hirabayashi , and Hiromitsu Nakauchi. Stem Cells and Development. 2012 Nov 1;21(16):2981-6.
• A Differentiation Checkpoint Limits Hematopoietic Stem Cell Self-Renewal in Response to DNA Damage. Wang J, Sun Q, Morita Y, Jiang H, Gros A, Lechel A, Hildner K, Guachalla LM, Gompf A, Hartmann D, Schambach A, Wuestefeld T, Dauch D, Schrezenmeier H, Hofmann WK, Nakauchi H, Ju Z, Kestler HA, Zender L, Rudolph KL. Cell. 2012 Mar 2;148(5):1001-1014.
• Lethal myelofibrosis induced by Bmi1-deficient hematopoietic cells unveils a tumor suppressor function of the polycomb group genes. Oguro H, Yuan J, Tanaka S, Miyagi S, Mochizuki-Kashio M, Ichikawa H, Yamazaki S, Koseki H, Nakauchi H, Iwama A. J Exp Med. 2012 Mar 12;209(3):445-54.
• Bmi1 confers resistance to oxidative stress on hematopoietic stem cells Shunsuke Nakamura, Motohiko Oshima, Jin Yuan, Atsunori Saraya, Satoru Miyagi, Takaaki Konuma1, Satoshi Yamazaki, Mitsujiro Osawa, Hiromitsu Nakauchi, Haruhiko Koseki, Atsushi Iwama, PLoS One. 2012;7(5):e36209. Epub 2012 May 11.
• Generation of Kidney from Pluripotent Stem Cells via Blastocyst Complementation. Jo-ichi Usui,Toshihiro Kobayashi, Tomoyuki Yamaguchi, A.S. Knisely Ryuichi Nishinakamura, Hiromitsu Nakauchi, Am J Pathol. 2012 Jun;180(6):2417-26.
• Ability of Tetraploid Rat Blastocysts to Support Fetal Development After Complementation With Embryonic Stem Cells. Masumi Hirabayashi, Chihiro Tamura, Makoto Sanbo, Teppei Goto, Megumi Kato-Itoh, Toshihiro Kobayashi, Hiromitsu Nakauchi and Shinichi Hochi.Mol. Reprod. Dev. 79: 402?412, 2012.
• Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells. Eliver Eid Bou Ghosna, Ryo Yamamoto, Sanae Hamanakab, Yang Yanga, Leonard A. Herzenberga, Hiromitsu Nakauchib, Leonore A. Herzenberg Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5394-8. Epub 2012 Mar 19.
• A retrospective analysis of germline competence in rat embryonic stem cell lines. Hirabayashi M, Tamura C, Sanbo M, Kato-Itoh M, Kobayashi T, Nakauchi H, Hochi S. Transgenic Res. 2012 Aug 9.[Epub ahead of print]
• Highly efficient generation of definitive endoderm lineage from human induced pluripotent stem cells. Sekine K, Takebe T, Suzuki Y, Kamiya A, Nakauchi H, Taniguchi H. Transplant Proc. 2012 May;44(4):1127-9.

2011

• Hair follicle stem cells provide a functional niche for melanocyte stem cells. Tanimura S, Tadokoro Y, Inomata K, Binh NT, Nishie W, Yamazaki S, Nakauchi H, Tanaka Y, McMillan JR, Sawamura D, Yancey K, Shimizu H, Nishimura EK. Cell Stem Cell, 2011 Feb 4;8(2):177-87.
• Development of Defective and Persistent Sendai Virus Vector: A Unique Gene Delivery/Expression System Ideal for Cell Reprograming. Nishimura, Ken; Sano, Masayuki; Ohtaka, Manami; Furuta, Birei; Umemura, Yoko; Nakajima, Yoshiro; Ikehara, Yuzuru; Kobayashi, Toshihiro; Segawa, Hiroaki; Takayasu, Satoko; Sato, Hideyuki; Motomura, Kaori; Uchida, Eriko; Kanayasu-Toyoda, Toshie; Asashima, Makoto; Nakauchi, Hiromitsu; Yamaguchi, Teruhide; Nakanishi, Mahito. Journal of Biological Chemistry, Vol. 286, No. 6 (February 11, 2011), 4760-4771, published online December 7, 2010
• Generation of Germline-Competent Rat Induced Pluripotent Stem Cells. Sanae Hamanaka, Tomoyuki Yamaguchi, Toshihiro Kobayashi, Megumi Kato-Itoh, Satoshi Yamazaki, Hideyuki Sato, Ayumi Umino, Yukiko Wakiyama, Mami Arai, Makoto Sanbo, Masumi Hirabayashi, Hiromitsu Nakauchi. PLoS ONE, Vol. 6, No. 7 (2011)
• Frequent pathway mutations of splicing machinery in myelodysplasia. Yoshida K, Sanada M, Shiraishi Y, Nowak D, Nagata Y, Yamamoto R, Sato Y, Sato-Otsubo A, Kon A, Nagasaki M, Chalkidis G, Suzuki Y, Shiosaka M, Kawahata R, Yamaguchi T, Otsu M, Obara N, Sakata-Yanagimoto M, Ishiyama K, Mori H, Nolte F, Hofmann WK, Miyawaki S, Sugano S, Haferlach C, Koeffler HP, Shih LY, Haferlach T, Chiba S, Nakauchi H, Miyano S, Ogawa S. Nature. 2011 Sep 11;478(7367): 64-9.
• Nonmyelinating Schwann cells in mouse bone marrow niche maintain hematopoietic stem cell hibernation through TGF-be-ta signaling. Satoshi Yamazaki, Hideo Ema, Goran Karlsson, ,Tomoyuki Yamaguchi, Hiroyuki Miyoshi, Seiji Shioda, Makoto M. Taketo, Stefan Karlsson, Atsushi Iwama, and Hiromitsu Nakauchi. Cell, 147, 1146-1158,2011.
• The creation of transgenic pigs expressing human proteins using BAC-derived, full-length genes and intracytoplasmic sperm injection-mediated gene transfer. Masahito Watanabe, Mayuko Kurome, Hitomi Matsunari, Kazuaki Nakano, Kazuhiro Umeyema, Akira Shiota, Hiromitsu Nakauchi, Hiroshi Nagashima. Transgenic Research, 2011 Oct 25. [Epub ahead of print]
• Characterization of the ICSI-mediated gene transfer method in the production of transgenic pigs. Kazuhiro Umeyama, Hitoshi Saito, Mayuko Kurome, Hitomi Matsunari, Masahito Watanabe, Hiromitsu Nakauchi, Hiroshi Nagashima. Molecular Reproduction and Development, 2012 Mar, 79 (3): 218-228.

2010

• Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells.　Toshihiro Kobayashi, Tomoyuki Yamaguchi, Sanae Hamanaka, Megumi Kato-Itoh, Yuji Yamazaki, Makoto Ibata, Hideyuki Sato,Youn-Su Lee,Jo-ichi Usui, A.S. Knisely, Masumi Hirabayashi, Hiromitsu Nakauchi. Cell 142, 787-799, 2010
• Rat Transgenesis Via Embryonic Stem Cells Electroporated With Kusabira-Orange Gene. Masumi Hirabayashi, Megumi Kato, Makoto Sanbo, Toshihiro Kobayashi, Shinichi Hochi, Hiromitsu Nakauchi. Mol Reprod Dev. 2010 Jun; 77(6):474,
• Establishment of rat embryunic stem cell lines that can participate in germline chimerae at high efficiency. Hirabayashi M, Kato M, Kobayashi T, Sanbo M, Yagi T, Hochi S, Nakauchi H. Mol Reprod Dev. 2010 Jun; 77(6):474,
• Poised lineage specification in multipotential hematopoietic stem and progenitor cells by the polycomb protein Bmi1. Hideaki Oguro, Jin Yan, Hitoshi Ichikawa, Tomokatsu Ikawa, Satoshi Yamazaki, Hiroshi Kawamoto, Hiromitsu Nakauchi, Atsushi Iwama. Cell Stem Cells,Vol6, 279-286, March 5, 2010
• TIM1 is an endogenous ligand for LMIR5/CD300b: LMIR5 deficiency ameliorates mouse kidney ischemia/reperfusion injury. Yoshinori Yamanishi , Jiro Kitaura, Kumi Izawa, Ayako Kaitani, Yukiko Komeno, Masaki Nakamura, Satoshi Yamazaki, Yutaka Enomoto, Toshihiko Oki, Hisaya Akiba, Takaya Abe, Tadasuke Komori, Yoshihiro Morikawa, Hiroshi Kiyonari6, Toshiyuki Takai, Ko Okumura, and Toshio Kitamura. The Journal of Experimental Medicine, Jul 5; 207(7): 1501-11.
• Knockout of exogenous EGFP gene in porcine somatic cells using zinc-finger nucleases. Watanabe M, Umeyama K, Matsunari H, Takayanagi S, Haruyama E, Nakano K, Fujiwara T, Ikezawa Y, Nakauchi H, Nagashima H. Biochemical and Biophysical Research Communications, 402, 14-18, 2010.

2009

• Hepatic Stem/Progenitor Cells and Stem Cell Transplantation for the Treatment of Liver Disease. Sei Kakinuma, Hiromitsu Nakauchi, Mamoru Watanabe. Journal of Gastroenterology 2009; 44:167-172
• Dominant-negative mutant hepatocyte nuclear factor 1･ induces diabetes in transgenic-cloned pigs. Kazuhiro Umeyama, Masahito Watanabe, Hitoshi Saito, Mayuko Kurome, Sadaaki Tohi, Hitomi Matsunari, Keizaburo Miki, Hiroshi Nagashima. Transgenic Research, Vol.18(5), p697-706, 2009
• Analyses of Cell Surface Molecules on Hepatic Stem/Progenitor Cells in Mouse Fetal Liver. Sei Kakinuma, Haruhiko Ohta, Akihide Kamiya, Yuji Yamazaki, Tsunekazu Oikawa, Ken Okada, Hiromitsu Nakauchi. Journal of Hepatology,　Vol.51, p127-138, 2009
• Application of genetically modified and cloned pigs in translational research. Hitomi Matsunari, Hiroshi Nagashima. Journal of Reproduction and Development, Vol.55(3), p225-230, 2009
• A rapid and efficient strategy to generate allele-specific anti-HLA monoclonal antibodies. Yamazaki S, Suzuki N, Saito T, Ishii Y, Takiguchi M, Nakauchi H, Watanabe N. Jornal of Immunol Methods　Vol. 343 No1
• TGF-beta as a candidate bone marrow niche signal to induce hematopoietic stem cell hibernation. Yamazaki S, Iwama A, Takayanagi S, Eto K, Ema H, Nakauchi H. Blood,　Vol.113,　No6, P1250-1256
• Definitive proof for direct reprogramming of hematopoietic cells to pluripotency. Motohito Okabe, Makoto Otsu, Ahn Dong Hyuck, Toshihiro Kobayashi, Yohei Morita, Yukiko Wakiyama, Masafumi Onodera, Koji Eto, Hideo Ema, Hiromitsu Nakauchi. Blood　114(9):1764-7.
• Gain-of-function of mutated C-CBL tumour suppressor in myeloid neoplasms. Sanada M, Suzuki T, Shih LY, Otsu M, Kato M, Yamazaki S, Tamura A, Honda H, Sakata-Yanagimoto M, Kumano K, Oda H, Yamagata T, Takita J, Gotoh N, Nakazaki K, Kawamata N, Onodera M, Nobuyoshi M, Hayashi Y, Harada H, Kurokawa M, Chiba S, Mori H, Ozawa K, Omine M, Hirai H, Nakauchi H, Koeffler HP, Ogawa S. Nature. 2009 13;460(7257):904-8.