Analyzing protein-protein interaction is essential to elucidate finely tuned life phenomena. Prof. Ueda and his co-workers have developed a novel technology, named “Firefly luminescent intermediate based Protein Interaction Assay (FlimPIA)”, to detect these interactions by combining two complementary luciferase mutants. Highly sensitive and specific, this technology demonstrated under-1-second detection of several known protein-protein interactions in a concentration-dependent manner.
FlimPIA may be applied to drug discovery and clinical diagnostics as a convenient, high speed and specific assay.
Grant Information
JST-SENTAN (Development of Advanced Measurement and Analysis Systems), Technology Development Type, “Development of novel protein-protein interaction detecting technology with high speed and sensitivity”
Journal Information
Yuki Ohmuro-Matsuyama, Kota Nakano, Aoi Kimura, Keiichi Ayabe, Masaki Ihara, Takeshi Wada, and Hiroshi Ueda.
“A Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases”
Published online on August 1st 2013, in “Analytical Chemistry”.
doi: 10.1021/ac4016825
Contact
[About Research]
Professor Hiroshi UEDA, Ph.D.
Chemical Resources Laboratory, Tokyo Institute of Technology
E-mail:
[About Program]
Akira KUBO
Masae SUGAWARA, Ph.D.
Department of Industry-Academic Alliance, Office of Advanced Measuring Technology,
Japan Science and Technology Agency
E-mail: