本文へジャンプ
技術参事
瀬古　玲
（SEKO Akira)
略　歴
1965年10月 三重県桑名市生まれ
1984年3月 三重県立桑名高等学校理数科　卒業
1984年4月 東京大学理科II類　入学
1988年3月 東京大学理学部生物化学科　卒業
1992年4月 日本学術振興会研究員
1993年3月 東京大学理学系大学院生物化学専攻博士課程　修了
1993年4月
株式会社太陽化学総合研究所　入社
（在職中、1993年6月～12月
理化学研究所国際フロンティア　出向）
1995年5月
株式会社太陽化学総合研究所　退社
財団法人佐々木研究所生化学部　入所
2002年8月
主任研究員
2006年3月
財団法人佐々木研究所生化学部　退所
2006年4月
東京工業大学イノベーション研究推進体研究員
2007年11月
東京工業大学特別研究員
2009年4月
東京工業大学非常勤講師
2010年6月
（独）科学技術振興機構ERATO伊藤グライコトリロジープロジェクト技術参事　現在に至る

受賞歴
第25回日本糖質学会奨励賞（2005年度）

所属学会
日本生化学会、日本糖質学会、日本癌学会、日本分子腫瘍マーカー学会

論　文
  1. Seko, A., Kitajima, K., Iwasaki, M., Inoue, S., and Inoue, Y. (1989) Structural studies of fertilization-associated carbohydrate-rich glycoproteins (hyosophorin) isolated from the fertilized and unfertilized eggs of flounder, Paralichthys olivaceus. Presence of a novel penta-antennary N-linked glycan chain in the tandem repeating glycopeptide unit of hyosophorin. J. Biol. Chem., 264, 15922-15929.
  2. Seko, A., Kitajima, K., Inoue, S., and Inoue, Y. (1991) Identification of free glycan chain liberated by de-N-glycosylation of the medaka fish, Oryzias latipes. Biochem. Biophys. Res. Commun., 180, 1165-1171.
  3. Seko, A., Kitajima, K., Inoue, Y., and Inoue, S. (1991) Peptide:N-glycosidase activity found in the early embryos of Oryzias latipes (medaka fish). The first demonstration of the occurrence of peptide:N-glycosidase in animal cells and its implication for the presence of a de-N-glycosylation system in living organisms. J. Biol. Chem., 266, 22110-22114.
  4. 大久保宏一、瀬古　玲、石井孝司、重松　亮、北島　健、井上康男、岩崎万理子、井上貞子、林不二雄、手島千里、佐藤敦彦、薩美賢策、奥本直人 (1991) 性成熟遅延系（３年成熟）雌ニジマスの卵表層胞糖タンパク質による同定 Nippon Suisan Gakkaishi, 57, 1355-1364.
  5. Shin, W.S., Sasaki, T., Kato, M., Hara, K., Seko, A., Yang, W-.D., Shimamoto, N., Sugimoto, T., and Toyo-oka, T. (1992) Autocrine and paracrine effects of endothelium-derived relaxing factor on intracellular Ca2+ of endothelial cells and vascular smooth muscle cells. Identification by two-dimensional image analysis in coculture. J. Biol. Chem., 267, 20377-20382.
  6. Iwasaki, M., Seko, A., Kitajima, K., Inoue, Y., and Inoue, S. (1992) Fish egg glycophosphoproteins have species-specific N-linked glycan units previously found in a storage pool of free glycan chains. J. Biol. Chem., 267, 24287-24296.
  7. Taguchi, T., Seko, A., Kitajima, K., Inoue, S., Iwamatsu, T., Khoo, K-.H., Morris, H.R., Dell, A., and Inoue, Y. (1993) Structural studies of a novel type of tetraantennary sialoglycan unit in a carbohydrate-rich glycopeptide isolated from the fertilized eggs of indian medaka fish, Oryzias melastigma. J. Biol. Chem., 268, 2353-2362.
  8. Suzuki, T., Seko, A., Kitajima, K., Inoue, Y., and Inoue, S. (1993) Identification of peptide:N-glycanase activity in mammalian derived cultured cells. Biochem. Biophys. Res. Commun., 194, 1124-1130.
  9. Taguchi, T., Seko, A., Kitajima, K., Muto, Y., Inoue, S., Khoo, K-.H., Morris, H.R., Dell, A., and Inoue, Y. (1994) Structural studies of a novel type of pentaantennary large glycan unit in the fertilization-associated carbohydrate-rich glycopeptide isolated from the fertilized eggs of Oryzias latipes. J. Biol. Chem., 269, 8762-8771.
  10. Suzuki, T., Seko, A., Kitajima, K., Inoue, Y., and Inoue, S. (1994) Purification and enzymatic properties of peptide:N-glycanase from C3H mouse-derived L-929 fibroblast cells. Possible widespread occurrence of post-translational remodification of proteins by N-deglycosylation. J. Biol. Chem., 269, 17611-17618.
  11. Koketsu, M., Seko, A., Juneja, L.R., Kim, M., Kashimura, N., and Yamamoto, T. (1995) An efficient preparation and structural characterization of sialylglycopeptides from protease treated egg yolk. J. Carbohydr. Chem., 14, 833-841.
  12. Seko, A., Ohkura, T., Kitamura, H., Yonezawa, S., Sato, E., and Yamashita, K. (1996) Quantitative differences in GlcNAc:b1→3 and GlcNAc:b1→4 galactosyltransferase activities between human colonic adenocarcinomas and normal colonic mucosa. Cancer Res., 56, 3468-3473.
  13. Fukushima, K., Hara-Kuge, S., Ohkura, T., Seko, A., Ideo, H., Inazu, T., and Yamashita, K. (1997) Lectin-like characteristics of recombinant human interleukin-1b recognizing glycans of glycosylphosphatidylinositol anchor. J. Biol. Chem., 272, 10579-10584.
  14. Seko, A., Juneja, L.R., and Yamamoto, T. (1997) Enzymes in unfertilized hen eggs. In Hen Eggs. Their Basic and Applied Science (Edited by Yamamoto, T., Juneja, L.R., Hatta, H., and Kim, M.) pp. 135-144. CRC Press, Inc., Boca Raton, Florida.
  15. Seko, A., and Juneja, L.R. (1997) Cell proliferation-promoting activities in unfertilized eggs. In Hen Eggs. Their Basic and Applied Science (Edited by Yamamoto, T., Juneja, L.R., Hatta, H., and Kim, M.) pp. 145-150. CRC Press, Inc., Boca Raton, Florida.
  16. Seko, A., Koketsu, M., Nishizono, M., Enoki, Y., Ibrahim, H.R., Juneja, L.R., Kim, M., and Yamamoto, T. (1997) Occurrence of a sialylglycopeptide and free sialylglycans in hen’s egg yolk. Biochim. Biophys. Acta, 1335, 23-32.
  17. Seko, A., Hara-Kuge, S., Yonezawa, S., Nagata, K., and Yamashita, K. (1998) Identification and characterization of N-acetylglucosamine-6-O-sulfate-specific b1,4-galactosyltransferase in human colorectal mucosa. FEBS Lett., 440, 307-310.
  18. Fukushima, K., Hara-Kuge, S., Seko, A., Ikehara, Y., and Yamashita, K. (1998) Elevation of Gal:a2→6 sialyltransferase and Gal:a1→2 fucosyltransferase activities in human choriocarcinoma. Cancer Res., 58, 4301-4306.
  19. Hara-Kuge, S., Ohkura, T., Seko, A., and Yamashita, K. (1999) Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing a1→2 mannosyl residues in MDCK cells. Glycobiology, 9, 833-839.
  20. Seko, A., Kitajima, K., Iwamatsu, T., Inoue, Y., and Inoue, S. (1999) Identification of two distinct peptide:N-glycanases in Oryzias latipes during embryogenesis. Glycobiology, 9, 887-895.
  21. Seko, A., Sumiya, J., Yonezawa, S., Nagata, K., and Yamashita, K. (2000) Biochemical differences in two types of N-acetylglucosamine:→6sulfotransferase between human colonic adenocarcinomas and the adjacent normal mucosa: specific expression of a GlcNAc:→6sulfotransferase in mucinous adenocarcinoma. Glycobiology, 10, 919-929.
  22. Seko, A., Hara-Kuge, S., and Yamashita, K. (2001) Molecular cloning and characterization of a novel human galactose 3-O-sulfotransferase that transfers sulfate to Galb1→3GalNAc residue in O-glycans. J. Biol. Chem., 276, 25697-25704.
  23. Ideo, H., Seko, A., Ohkura, T., Matta, K.L., and Yamashita, K. (2002) High affinity binding of recombinant human galectin-4 to SO3-→3Galb1→3GalNAc pyranoside.Glycobiology, 12, 199-208.
  24. Seko, A., Nagata, K., Yonezawa, S., and Yamashita, K. (2002) Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic nucinous adenocarcinoma. Glycobiology, 12, 379-388.
  25. Seko, A., Nagata, K., Yonezawa, S., and Yamashita, K. (2002) Down-regulation of Gal 3-O-sulfotransferase-2 (Gal3ST-2) expression in human colonic non-mucinous adenocarcinoma. Jpn. J. Cancer Res., 93, 507-515.
  26. Ohkura, T., Seko, A., Hara-Kuge, S., and Yamashita, K. (2002) Occurrence of secretory glycoprotein-specific GalNAcb1®4GlcNAc sequence in N-glycans in MDCK cells. J. Biochem. (Tokyo), 132, 891-901.
  27. Hara-Kuge, S., Seko, A., and Yamashita, K. (2003) Carbohydrate recognition of vesicular integral protein of 36 kDa(VIP36) in intracellular transport of newly synthesized glycoproteins. Methods Enzymol., 363, 525-532.
  28. Seko, A., Dohmae, N., Takio, K., and Yamashita, K. (2003) b1,4-galactosyltransferase (b4GalT)-IV is specific for GlcNAc 6-O-sulfate. b4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X. J. Biol. Chem., 278, 9150-9158.
  29. Ideo, H., Seko, A., Ishizuka, I., and Yamashita, K. (2003) The N-terminal carbohydrate recognition domain of galectin-8 recognizes specific glycosphingolipids with high-affinity. Glycobiology, 13, 713-723.
  30. Seko, A., and Yamashita, K.(2004) b1,3-N-acetylglucosaminyltransferase-7(b3Gn-T7) acts efficiently on keratan sulfate-related glycans. FEBS Lett., 556, 216-220.
  31. Hara-Kuge, S., Seko, A., Shimada, O., Tosaka-Shimada, H., and Yamashita, K. (2004) The binding of VIP36 and a-amylase in the secretory vesicles via high-mannose type glycans. Glycobiology, 14, 739-744.
  32. Ideo, H., Seko, A., and Yamashita, K. (2005) Galectin-4 binds to sulfated glycosphingolipids and Carcinoembryonic antigen in patches on the cell surface of human colon adenocarcinoma cells. J. Biol. Chem., 280, 4730-4737.
  33. Seko, A., and Yamashita, K. (2005) Identification and characterization of a novel galactose:b1,3-N-acetylglucosaminyltransferase (b3Gn-T8): The complex formation of b3Gn-T2 and b3Gn-T8 enhances enzymatic activity. Glycobiology 15, 943-951.
  34. Seko, A., Sumiya, J., and Yamashita, K. (2005) Porcine, mouse, and human Gal 3-O-sulfotransferase-2 enzymes have different substrate specificities; the porcine enzyme requires basic compounds for its catalytic activity. Biochemical Journal 391, 77-85.
  35. Kanoh, A., Seko, A., Ideo, H., Yoshida, M., Nomoto, M., Yonezawa, S., Sakamoto, M., Kannagi, R., and Yamashita, K. (2006) Ectopic expression of N-acetylglucosamine 6-O-sulfotransferase 2 in chemotherapy-resistant ovarian adenocarcinomas. Glycoconjugate J., 23, 453-460, 2006.
  36. Dejima, K., Seko, A., Yamashita, K., Gengyo-Ando, K., Mitani, S., Izumikawa, T., Kitagawa, H., Sugahara, K., Mizuguchi, S., and Nomura, K. (2006) Essential roles of 3'-phosphoadenosine 5'-phosphosulfate synthase in embryonic and larval development of the nematode Caenorhabditis elegans. J. Biol. Chem., 281, 11431-11440.
  37. Ideo, H., Seko, A., and Yamashita, K. (2007) Recognition Mechanism of Galectin-4 for Cholesterol 3-Sulfate. J. Biol. Chem., 282, 21081-21089.
  38. Seko, A., and Yamashita, K. (2008) Activation of b1,3-N-Acetylglucosaminyltransferase-2 (b3Gn-T2) by b3Gn-T8: Possible involvement of b3Gn-T8 in Increasing Poly-N-Acetyllactosamine Chains in Differentiated HL-60 Cells. J. Biol. Chem., 283, 33094-33100.
  39. Seko, A., Kataoka, F., Aoki, D., Sakamoto, M., Nakamura, T., Hatae, M., Yonezawa, S., and Yamashita, K. (2009) N-acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer. Glycoconjugate J., in press (PMID: 19156517).
  40. Seko, A., Kataoka, F., Aoki, D., Sakamoto, M., Nakamura, T., Hatae, M., Yonezawa, S., and Yamashita, K. (2009) b1,3-Galactosyltransferases-4/5 are novel tumor markers for gynecological cancers. Tumor Biol., 30, 43-50.
  41. Seko, A., Yamase, T., and Yamashita, K. (2009) Polyoxometalates as effective inhibitors for sialyl- and sulfotransferases. J. Inorg. Biochem., 103, 1061-1066.
  42. Masayuki Izumi, Yutaka Makimura, Simone Dedola, Akira Seko, Akiko Kanamori, Masafumi Sakono, Yukishige Ito, and Yasuhiro Kajihara. (2012) Chemical Synthesis of Intentionally Misfolded Homogeneous Glycoprotein: A Unique Approach for the Study of Glycoprotein Quality Control. J. Am. Chem. Soc., 134, 7238-7241.
  43. Masafumi Sakono, Akira Seko, Yoichi Takeda, Masakazu Hachisu, and Yukishige Ito. (2012) Biophysical properties of UDP-glucose:glycoprotein glucosyltransferase, a folding sensor enzyme in the ER, delineated by synthetic probes. BBRC, 426, 504-510.
  44. Yoichi Takeda, Akira Seko, Masafumi Sakono, Masakazu Hachisu, Akihiko Koizumi, Kohki Fujikawa, and Yukishige Ito (2013) Parallel quantification of lectin–glycan interaction using ultrafiltration. Carbohydrate Research, 375, 112-117.
  45. Akihiko Koizumi, Ichiro Matsuo, Maki Takatani, Akira Seko, Masakazu Hachisu, Yoichi Takeda, and Yukishige Ito. (2013) Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming. Angewandte Chemie, 52, 7426–7431.
  46. H. Ideo, T. Matsuzaka, T. Nonaka, A. Seko and K. Yamashita. (2011) Galectin-8-N-domain Recognition Mechanism for Sialylated and Sulfated Glycans. J.Biol.Chem., 286, 11346-11355.
  47. A. Seko, T. Ohkura, H. Ideo and K.Yamashita (2012) Novel O-linked glycans containing 6′-sulfo-Gal/GalNAc of MUC1 secreted from human breast cancer YMB-S cells: Possible carbohydrate epitopes of KL-6(MUC1) monoclonal antibody. Glycobiology, 22, 181-195.
  48. A. Koizumi, I. Matsuo, M. Takatani, A. Seko, M. Hachisu, Y. Takeda, Y. Ito. (2013) Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming. Angewandte Chemie, 52, 7426-7431.
  49. Y. Takeda, A. Seko, M. Sakono, M. Hachisu, A. Koizumi, K. Fujikawa, Y. Ito. (2013) Parallel quantification of lectin–glycan interaction using ultrafiltration. Carbohydrate Res. 375, 112-117.
  50. Y. Takeda, A. Seko, M. Hachisu, S. Daikoku, M. Izumi, A. Koizumi, K. Fujikawa, Y. Kajihara, and Y. Ito. (2014) Both Isoforms of Human UDP-glucose:glycoprotein Glucosyltransferase are Enzymatically Active. Glycobiology 24, 344-350.
  51. S. Dedola, M. Izumi, Y. Makimura, A. Seko, A. Kanamori, M. Sakono, Y. Ito, Y. Kajihara. (2014) Folding of Synthetic Homogeneous Glycoproteins in the Presence of a Glycoprotein Folding Sensor Enzyme. Angewandte Chemie International Edition 53, 2883-2887.
  52. M. Sakono, A. Seko, Y. Takeda, J. Aikawa, M. Hachisu, A. Koizumi, K. Fujikawa, Y. Ito. (2014) Glycan specificity of a testis-specific lectin chaperone calmegin and effects of hydrophobic interactions. Biochimica et Biophysica Acta (BBA) - General Subjects 1840, 2904-2913.
  53. S. Iwamoto, Y. Kasahara, K. Kamei, A. Seko, Y. Takeda, Y. Ito, I. Matsuo (2014) Measurement of endo-α-mannosidase activity using a fluorescently labeled oligosaccharide derivatives. Biosci. Biotech. Biochem. 78, 927-936.
  54. S. Daikoku, A. Seko, Y. Ito, O. Kanie. (2014) Glycan structure and site of glycosylation in the ER-resident glycoprotein, uridine 5'-diphosphate-glucose: glycoprotein glucosyltransferases 1 from rat, porcine, bovine, and human. Biochem. Biophys. Res. Commun. 451, 356-360.
  55. M. Sakono, A. Seko, Y. Takeda, Y. Ito. (2014) PDI family protein ERp29 forms 1:1 complex with lectin chaperone calreticulin. Biochem. Biophys. Res. Commun. 452, 27-31.
  56. K. Fujikawa, A. Koizumi, M. Hachisu, A. Seko, Y. Takeda, Y. Ito. (2015) Construction of a High-Mannose-Type Glycan Library by a Renewed Top-Down Chemo-Enzymatic Approach. Biochem. Biophys. Res. Commun. 21, 3224-3233.
総　説
  1. Seko, A. (2006) Complex formation of glycosyltransferases and their biological significance. (review) Trends Glycosci. Glycotechnol., 18, 209-230.
  2. 伊藤 幸成,　金森 審子,　瀬古 玲,　武田 陽一 “化学合成をツールとする糖タンパク質の小胞体内機能解析” 化学工業 62, 65-72 (2011)
  3. Takeda, Y., Seko, A., and Ito, Y. (2014) Functional Analysis of Endoplasmic Reticulum Glucosyltransderase (UGGT) Using Synthetic Glycans. (review) Trends Glycosci. Glycotechnol., 26, 107-118.

趣味・特技：
10年ほど前までは、山登り、サイクリング、映画（ミニシアター系）、テニス、日本酒、洋楽CDあさり、などをやっていましたが、いずれもご無沙汰です。最近は読書（歴史物）と新聞の将棋欄を見ることが楽しみです。また、子供の教育も楽しんで（頭痛のタネ？）やっています。

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