本文へジャンプ
Research Manager
SEKO Akira
Education and Experience
Education
1988.3
Graduated at the Faculty of Science, University of Tokyo
1993.3
Obtained Ph.D. at Dr. Yasuo Inoue’s laboratory, Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo
Experience
1993.4
Engaged in the Research Institute of Taiyo Chemical Company
(Transiently moved to Dr. C.H. Wong and Dr. T. Kajimoto’s laboratory at RIKEN)
1995.5
Moved to the Division of Biochemistry, Sasaki Institute (Dr. Katsuko Yamashita’s laboratory)
2006.4
Moved to Innovative Research Initiatives, Tokyo Institute of Technology (NEDO)
2010.6
Moved to JST ERATO Ito Glycotrilogy Project at RIKEN (Dr. Yukishige Ito’s laboratory) as Research Manager

Award
25th the Encouragement Awards of Japanese Society of Carbohydrate Research (2005)

Academic Society
The Japanese Biochemical Society
The Japanese Society of Carbohydrate Research
Japanese Cancer Association
Japan Society for Molecular Tumor Maker Research

Publications
  1. Seko, A., Kitajima, K., Iwasaki, M., Inoue, S., and Inoue, Y. (1989) Structural studies of fertilization-associated carbohydrate-rich glycoproteins (hyosophorin) isolated from the fertilized and unfertilized eggs of flounder, Paralichthys olivaceus. Presence of a novel penta-antennary N-linked glycan chain in the tandem repeating glycopeptide unit of hyosophorin. J. Biol. Chem., 264, 15922-15929.
  2. Seko, A., Kitajima, K., Inoue, S., and Inoue, Y. (1991) Identification of free glycan chain liberated by de-N-glycosylation of the medaka fish, Oryzias latipes. Biochem. Biophys. Res. Commun., 180, 1165-1171.
  3. Seko, A., Kitajima, K., Inoue, Y., and Inoue, S. (1991) Peptide:N-glycosidase activity found in the early embryos of Oryzias latipes (medaka fish). The first demonstration of the occurrence of peptide:N-glycosidase in animal cells and its implication for the presence of a de-N-glycosylation system in living organisms. J. Biol. Chem., 266, 22110-22114.
  4. Shin, W.S., Sasaki, T., Kato, M., Hara, K., Seko, A., Yang, W-.D., Shimamoto, N., Sugimoto, T., and Toyo-oka, T. (1992) Autocrine and paracrine effects of endothelium-derived relaxing factor on intracellular Ca2+ of endothelial cells and vascular smooth muscle cells. Identification by two-dimensional image analysis in coculture. J. Biol. Chem., 267, 20377-20382.
  5. Iwasaki, M., Seko, A., Kitajima, K., Inoue, Y., and Inoue, S. (1992) Fish egg glycophosphoproteins have species-specific N-linked glycan units previously found in a storage pool of free glycan chains. J. Biol. Chem., 267, 24287-24296.
  6. Taguchi, T., Seko, A., Kitajima, K., Inoue, S., Iwamatsu, T., Khoo, K-.H., Morris, H.R., Dell, A., and Inoue, Y. (1993) Structural studies of a novel type of tetraantennary sialoglycan unit in a carbohydrate-rich glycopeptide isolated from the fertilized eggs of indian medaka fish, Oryzias melastigma. J. Biol. Chem., 268, 2353-2362.
  7. Suzuki, T., Seko, A., Kitajima, K., Inoue, Y., and Inoue, S. (1993) Identification of peptide:N-glycanase activity in mammalian derived cultured cells. Biochem. Biophys. Res. Commun., 194, 1124-1130.
  8. Taguchi, T., Seko, A., Kitajima, K., Muto, Y., Inoue, S., Khoo, K-.H., Morris, H.R., Dell, A., and Inoue, Y. (1994) Structural studies of a novel type of pentaantennary large glycan unit in the fertilization-associated carbohydrate-rich glycopeptide isolated from the fertilized eggs of Oryzias latipes. J. Biol. Chem., 269, 8762-8771.
  9. Suzuki, T., Seko, A., Kitajima, K., Inoue, Y., and Inoue, S. (1994) Purification and enzymatic properties of peptide:N-glycanase from C3H mouse-derived L-929 fibroblast cells. Possible widespread occurrence of post-translational remodification of proteins by N-deglycosylation. J. Biol. Chem., 269, 17611-17618.
  10. Koketsu, M., Seko, A., Juneja, L.R., Kim, M., Kashimura, N., and Yamamoto, T. (1995) An efficient preparation and structural characterization of sialylglycopeptides from protease treated egg yolk. J. Carbohydr. Chem., 14, 833-841.
  11. Seko, A., Ohkura, T., Kitamura, H., Yonezawa, S., Sato, E., and Yamashita, K. (1996) Quantitative differences in GlcNAc:b1→3 and GlcNAc:b1→4 galactosyltransferase activities between human colonic adenocarcinomas and normal colonic mucosa. Cancer Res., 56, 3468-3473.
  12. Fukushima, K., Hara-Kuge, S., Ohkura, T., Seko, A., Ideo, H., Inazu, T., and Yamashita, K. (1997) Lectin-like characteristics of recombinant human interleukin-1b recognizing glycans of glycosylphosphatidylinositol anchor. J. Biol. Chem., 272, 10579-10584.
  13. Seko, A., Juneja, L.R., and Yamamoto, T. (1997) Enzymes in unfertilized hen eggs. In Hen Eggs. Their Basic and Applied Science (Edited by Yamamoto, T., Juneja, L.R., Hatta, H., and Kim, M.) pp. 135-144. CRC Press, Inc., Boca Raton, Florida.
  14. Seko, A., and Juneja, L.R. (1997) Cell proliferation-promoting activities in unfertilized eggs. In Hen Eggs. Their Basic and Applied Science (Edited by Yamamoto, T., Juneja, L.R., Hatta, H., and Kim, M.) pp. 145-150. CRC Press, Inc., Boca Raton, Florida.
  15. Seko, A., Koketsu, M., Nishizono, M., Enoki, Y., Ibrahim, H.R., Juneja, L.R., Kim, M., and Yamamoto, T. (1997) Occurrence of a sialylglycopeptide and free sialylglycans in hen’s egg yolk. Biochim. Biophys. Acta, 1335, 23-32.
  16. Seko, A., Hara-Kuge, S., Yonezawa, S., Nagata, K., and Yamashita, K. (1998) Identification and characterization of N-acetylglucosamine-6-O-sulfate-specific b1,4-galactosyltransferase in human colorectal mucosa. FEBS Lett., 440, 307-310.
  17. Fukushima, K., Hara-Kuge, S., Seko, A., Ikehara, Y., and Yamashita, K. (1998) Elevation of Gal:a2→6 sialyltransferase and Gal:a1→2 fucosyltransferase activities in human choriocarcinoma. Cancer Res., 58, 4301-4306.
  18. Hara-Kuge, S., Ohkura, T., Seko, A., and Yamashita, K. (1999) Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing a1→2 mannosyl residues in MDCK cells. Glycobiology, 9, 833-839.
  19. Seko, A., Kitajima, K., Iwamatsu, T., Inoue, Y., and Inoue, S. (1999) Identification of two distinct peptide:N-glycanases in Oryzias latipes during embryogenesis. Glycobiology, 9, 887-895.
  20. Seko, A., Sumiya, J., Yonezawa, S., Nagata, K., and Yamashita, K. (2000) Biochemical differences in two types of N-acetylglucosamine:→6sulfotransferase between human colonic adenocarcinomas and the adjacent normal mucosa: specific expression of a GlcNAc:→6sulfotransferase in mucinous adenocarcinoma. Glycobiology, 10, 919-929.
  21. Seko, A., Hara-Kuge, S., and Yamashita, K. (2001) Molecular cloning and characterization of a novel human galactose 3-O-sulfotransferase that transfers sulfate to Galb1→3GalNAc residue in O-glycans. J. Biol. Chem., 276, 25697-25704.
  22. Ideo, H., Seko, A., Ohkura, T., Matta, K.L., and Yamashita, K. (2002) High affinity binding of recombinant human galectin-4 to SO3-→3Galb1→3GalNAc pyranoside.Glycobiology, 12, 199-208.
  23. Seko, A., Nagata, K., Yonezawa, S., and Yamashita, K. (2002) Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic nucinous adenocarcinoma. Glycobiology, 12, 379-388.
  24. Seko, A., Nagata, K., Yonezawa, S., and Yamashita, K. (2002) Down-regulation of Gal 3-O-sulfotransferase-2 (Gal3ST-2) expression in human colonic non-mucinous adenocarcinoma. Jpn. J. Cancer Res., 93, 507-515.
  25. Ohkura, T., Seko, A., Hara-Kuge, S., and Yamashita, K. (2002) Occurrence of secretory glycoprotein-specific GalNAcb1®4GlcNAc sequence in N-glycans in MDCK cells. J. Biochem. (Tokyo), 132, 891-901.
  26. Hara-Kuge, S., Seko, A., and Yamashita, K. (2003) Carbohydrate recognition of vesicular integral protein of 36 kDa(VIP36) in intracellular transport of newly synthesized glycoproteins. Methods Enzymol., 363, 525-532.
  27. Seko, A., Dohmae, N., Takio, K., and Yamashita, K. (2003) b1,4-galactosyltransferase (b4GalT)-IV is specific for GlcNAc 6-O-sulfate. b4GalT-IV acts on keratan sulfate-related glycans and a precursor glycan of 6-sulfosialyl-Lewis X. J. Biol. Chem., 278, 9150-9158.
  28. Ideo, H., Seko, A., Ishizuka, I., and Yamashita, K. (2003) The N-terminal carbohydrate recognition domain of galectin-8 recognizes specific glycosphingolipids with high-affinity. Glycobiology, 13, 713-723.
  29. Seko, A., and Yamashita, K.(2004) b1,3-N-acetylglucosaminyltransferase-7(b3Gn-T7) acts efficiently on keratan sulfate-related glycans. FEBS Lett., 556, 216-220.
  30. Hara-Kuge, S., Seko, A., Shimada, O., Tosaka-Shimada, H., and Yamashita, K. (2004) The binding of VIP36 and a-amylase in the secretory vesicles via high-mannose type glycans. Glycobiology, 14, 739-744.
  31. Ideo, H., Seko, A., and Yamashita, K. (2005) Galectin-4 binds to sulfated glycosphingolipids and Carcinoembryonic antigen in patches on the cell surface of human colon adenocarcinoma cells. J. Biol. Chem., 280, 4730-4737.
  32. Seko, A., and Yamashita, K. (2005) Identification and characterization of a novel galactose:b1,3-N-acetylglucosaminyltransferase (b3Gn-T8): The complex formation of b3Gn-T2 and b3Gn-T8 enhances enzymatic activity. Glycobiology 15, 943-951.
  33. Seko, A., Sumiya, J., and Yamashita, K. (2005) Porcine, mouse, and human Gal 3-O-sulfotransferase-2 enzymes have different substrate specificities; the porcine enzyme requires basic compounds for its catalytic activity. Biochemical Journal 391, 77-85.
  34. Kanoh, A., Seko, A., Ideo, H., Yoshida, M., Nomoto, M., Yonezawa, S., Sakamoto, M., Kannagi, R., and Yamashita, K. (2006) Ectopic expression of N-acetylglucosamine 6-O-sulfotransferase 2 in chemotherapy-resistant ovarian adenocarcinomas. Glycoconjugate J., 23, 453-460, 2006.
  35. Dejima, K., Seko, A., Yamashita, K., Gengyo-Ando, K., Mitani, S., Izumikawa, T., Kitagawa, H., Sugahara, K., Mizuguchi, S., and Nomura, K. (2006) Essential roles of 3'-phosphoadenosine 5'-phosphosulfate synthase in embryonic and larval development of the nematode Caenorhabditis elegans. J. Biol. Chem., 281, 11431-11440.
  36. Ideo, H., Seko, A., and Yamashita, K. (2007) Recognition Mechanism of Galectin-4 for Cholesterol 3-Sulfate. J. Biol. Chem., 282, 21081-21089.
  37. Seko, A., and Yamashita, K. (2008) Activation of b1,3-N-Acetylglucosaminyltransferase-2 (b3Gn-T2) by b3Gn-T8: Possible involvement of b3Gn-T8 in Increasing Poly-N-Acetyllactosamine Chains in Differentiated HL-60 Cells. J. Biol. Chem., 283, 33094-33100.
  38. Seko, A., Kataoka, F., Aoki, D., Sakamoto, M., Nakamura, T., Hatae, M., Yonezawa, S., and Yamashita, K. (2009) N-acetylglucosamine 6-O-sulfotransferase-2 as a tumor marker for uterine cervical and corpus cancer. Glycoconjugate J., in press (PMID: 19156517).
  39. Seko, A., Kataoka, F., Aoki, D., Sakamoto, M., Nakamura, T., Hatae, M., Yonezawa, S., and Yamashita, K. (2009) b1,3-Galactosyltransferases-4/5 are novel tumor markers for gynecological cancers. Tumor Biol., 30, 43-50.
  40. Seko, A., Yamase, T., and Yamashita, K. (2009) Polyoxometalates as effective inhibitors for sialyl- and sulfotransferases. J. Inorg. Biochem., 103, 1061-1066.
  41. Masayuki Izumi, Yutaka Makimura, Simone Dedola, Akira Seko, Akiko Kanamori, Masafumi Sakono, Yukishige Ito, and Yasuhiro Kajihara. (2012) Chemical Synthesis of Intentionally Misfolded Homogeneous Glycoprotein: A Unique Approach for the Study of Glycoprotein Quality Control. J. Am. Chem. Soc., 134, 7238-7241.
  42. Masafumi Sakono, Akira Seko, Yoichi Takeda, Masakazu Hachisu, and Yukishige Ito. (2012) Biophysical properties of UDP-glucose:glycoprotein glucosyltransferase, a folding sensor enzyme in the ER, delineated by synthetic probes. BBRC, 426, 504-510.
  43. Yoichi Takeda, Akira Seko, Masafumi Sakono, Masakazu Hachisu, Akihiko Koizumi, Kohki Fujikawa, and Yukishige Ito (2013) Parallel quantification of lectin–glycan interaction using ultrafiltration. Carbohydrate Research, 375, 112-117.
  44. Akihiko Koizumi, Ichiro Matsuo, Maki Takatani, Akira Seko, Masakazu Hachisu, Yoichi Takeda, and Yukishige Ito. (2013) Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming. Angewandte Chemie, 52, 7426–7431.
  45. H. Ideo, T. Matsuzaka, T. Nonaka, A. Seko and K. Yamashita. (2011) Galectin-8-N-domain Recognition Mechanism for Sialylated and Sulfated Glycans. J.Biol.Chem., 286, 11346-11355.
  46. A. Seko, T. Ohkura, H. Ideo and K.Yamashita (2012) Novel O-linked glycans containing 6′-sulfo-Gal/GalNAc of MUC1 secreted from human breast cancer YMB-S cells: Possible carbohydrate epitopes of KL-6(MUC1) monoclonal antibody. Glycobiology, 22, 181-195.
  47. A. Koizumi, I. Matsuo, M. Takatani, A. Seko, M. Hachisu, Y. Takeda, Y. Ito. (2013) Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming. Angewandte Chemie, 52, 7426-7431.
  48. Y. Takeda, A. Seko, M. Sakono, M. Hachisu, A. Koizumi, K. Fujikawa, Y. Ito. (2013) Parallel Quantification of Lectin–Glycan Interaction using Ultrafiltration. Carbohydrate Res. 375, 112-117.
  49. Y. Takeda, A. Seko, M. Hachisu, S. Daikoku, M. Izumi, A. Koizumi, K. Fujikawa, Y. Kajihara, and Y. Ito. (2014) Both Isoforms of Human UDP-glucose:glycoprotein Glucosyltransferase are Enzymatically Active. Glycobiology 24, 344-350.
  50. S. Dedola, M. Izumi, Y. Makimura, A. Seko, A. Kanamori, M. Sakono, Y. Ito, Y. Kajihara. (2014) Folding of Synthetic Homogeneous Glycoproteins in the Presence of a Glycoprotein Folding Sensor Enzyme. Angewandte Chemie International Edition 53, 2883-2887.
  51. M. Sakono, A. Seko, Y. Takeda, J. Aikawa, M. Hachisu, A. Koizumi, K. Fujikawa, Y. Ito. (2014) Glycan specificity of a testis-specific lectin chaperone calmegin and effects of hydrophobic interactions. Biochimica et Biophysica Acta (BBA) - General Subjects 1840, 2904-2913.
  52. S. Iwamoto, Y. Kasahara, K. Kamei, A. Seko, Y. Takeda, Y. Ito, I. Matsuo (2014) Measurement of endo-α-mannosidase activity using a fluorescently labeled oligosaccharide derivatives. Biosci. Biotech. Biochem. 78, 927-936.
  53. S. Daikoku, A. Seko, Y. Ito, O. Kanie. (2014) Glycan structure and site of glycosylation in the ER-resident glycoprotein, uridine 5'-diphosphate-glucose: glycoprotein glucosyltransferases 1 from rat, porcine, bovine, and human. Biochem. Biophys. Res. Commun. 451, 356-360.
  54. M. Sakono, A. Seko, Y. Takeda, Y. Ito. (2014) PDI family protein ERp29 forms 1:1 complex with lectin chaperone calreticulin. Biochem. Biophys. Res. Commun. 452, 27-31.
  55. K. Fujikawa, A. Koizumi, M. Hachisu, A. Seko, Y. Takeda, Y. Ito. (2015) Construction of a High-Mannose-Type Glycan Library by a Renewed Top-Down Chemo-Enzymatic Approach. Biochem. Biophys. Res. Commun. 21, 3224-3233.
Review
  1. Seko, A. (2006) Complex formation of glycosyltransferases and their biological significance. (review) Trends Glycosci. Glycotechnol., 18, 209-230.
  2. Takeda, Y., Seko, A., Ito, Y. (2014) Functional Analysis of Endoplasmic Reticulum Glucosyltransderase (UGGT) Using Synthetic Glycans. (review) Trends Glycosci. Glycotechnol., 26, 107-118.

Miscellaneous:
About ten years ago, I liked climbing, cycling, watching movies, drinking sake, and playing tennis, but now I have little done them (lack of outdoor exercise !). Recently, my hobby is reading historical books, Japanese chess, and education of my daughters. The last is sometimes a cause of headache.


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