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JST Press Release

Dec. 16, 2013
Japan Science and Technology Agency (JST)
5-3, Yonbancho, Chiyoda-ku, Tokyo 102-8666
URL http://www.jst.go.jp/EN/index.html

Development of a new selective labeling method for the target protein of bioactive molecule
by means of turn-ON fluorescence

Target identification of bioactive molecules, which leads to the development of novel classes of drugs with few side effects or the discovery of new druggable proteins, is a vital and still daunting task. To overcome the time-consuming work, we developed a novel affinity labeling method employing a small bifunctional O-nitrobenzoxadiazole (O-NBD) unit having both reactivity and potential fluorescence. O-NBD is usually not fluorescent, but gives the fluorescence in response to existing amino group in protein. This method is not only simple but also powerful for target identification as well as ligand binding-site determination. Moreover, since the target labeling takes place with turn-ON fluorescence, the approach enables a sensitive imaging/detection of target proteins in living cells.

Researcher Information

The Exploratory Research for Advanced Technology (ERATO)
"Sodeoka Live Cell Chemistry Project"

Journal Information

Takao Yamaguchi, Miwako Asanuma, Shuichi Nakanishi, Yohei Saito,
Masateru Okazaki, Kosuke Dodo, and Mikiko Sodeoka
"Turn-ON fluorescent affinity labeling using a small bifunctional O-nitorobenzoxadiazole unit"
Chem. Sci., 2014, Advance Article
doi: 10.1039/C3SC52704B


[About Research]
Mikiko Sodeoka Ph.D.
Chief Scientist
Director of Synthetic Organic Chemistry, RIKEN
Director of ERATO Live Cell Chemistry Project, JST

[About Program]
Yoshizumi Sakamoto
Department of Research Project
Japan Science and Technology Agency


JST, an integrated organization of science and technology in Japan, establishes an infrastructure for the entire process from the creation of knowledge to the return to the society. For more information, visit http://www.jst.go.jp/EN/