Large scale expression of active enzymes is essential for their industrial use. However, insoluble enzymes are often expressed in the Escherichia coli expression system and hinder the progress of both basic and applied research of enzymes. To assign aggregation hot-spots for soluble expression, two rules were found: the α-helix rule, which focuses on the hydrophobicity of amino acids in the α-helix structure, and the hydropathy contradiction rule, which focuses on the difference in hydrophobicity compared to the amino acid in the consensus protein. Both the rules are attractive to the rational design of the expression of soluble enzymes.
ASANO Active Enzyme Molecule Project
Daisuke Matsui, Shogo Nakano, Mohammad Dadashipour and Yasuhisa Asano. “Rational identification of aggregation hotspots based on secondary structure and amino acid hydrophobicity”. Scientific Reports, Published online 25 August 2017, doi: 10.1038/s41598-017-09749-2
Yasuhisa Asano, Ph.D.
Professor, Toyama Prefectural University
Department of Research Project, JST