Shibata M, Nishimasu H, Kodera N, Hirano S, Ando T, Uchihashi T, Nureki O.
Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy.
Nat Commun. 2017 Nov 10;8(1):1430.
Yamano T, Zetsche B, Ishitani R, Zhang F, Nishimasu H, Nureki O.
Structural basis for the canonical and non-canonical PAM recognition by CRISPR-Cpf1.
Mol Cell. 2017 Aug 17;67(4):633-645.e3.
Nishimasu H, Yamano T, Gao L, Zhang F, Ishitani R, Nureki O.
Structural basis for the altered PAM recognition by engineered CRISPR-Cpf1.
Mol Cell. 2017 Jul 6;67(1):139-147.e2.
Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, Nishimasu H, Nureki O, Crosetto N, Zhang F.
Engineered Cpf1 variants with altered PAM specificities.
Nat Biotechnol. 2017 Aug;35(8):789-792.
Yamada M, Watanabe Y, Gootenberg JS, Hirano H, Ran FA, Nakane T, Ishitani R, Zhang F, Nishimasu H, Nureki O.
Crystal structure of the minimal Cas9 from Campylobacter jejuni reveals the molecular diversity in the CRISPR-Cas9 systems.
Mol Cell. 2017 Mar 16;65(6):1109-1121.
Matsumoto N, Nishimasu H, Sakakibara K, Nishida KM, Hirano T, Ishitani R, Siomi H, Siomi MC, Nureki O.
Crystal structure of silkworm PIWI-clade Argonaute Siwi bound to piRNA.
Cell. 2016 Oct 6;167(2):484-497.
Kato K, Satouh Y, Nishimasu H, Kurabayashi A, Morita J, Fujihara Y, Oji A, Ishitani R, Ikawa M, Nureki O
Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization.
Nat Commun. 2016 Jul 15;7:12198.
Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker IM, Li Y, Fedorova I, Nakane T, Makarova KS, Koonin EV, Ishitani R, Zhang F, Nureki O.
Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA.
Cell. 2016 May 5;165(4):949-62.
Hirano S, Nishimasu H, Ishitani R, Nureki O. Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9. Mol Cell. 2016 Mar 17;61(6):886-94. doi: 10.1016/j.molcel.2016.02.018
Hirano H, Gootenberg JS, Horii T, Abudayyeh OO, Kimura M, Hsu PD, Nakane T, Ishitani R, Hatada I, Zhang F, Nishimasu H, Nureki O. Structure and Engineering of Francisella novicida Cas9. Cell. 2016 Feb 25;164(5):950-61. doi: 10.1016/j.cell.2016.01.039. Epub 2016 Feb 11
Nishimasu H, Cong L, Yan WX, Ran FA, Zetsche B, Li Y, Kurabayashi A, Ishitani R, Zhang F, Nureki O.:Crystal structure of Staphylococcus aureus Cas9. Cell. 2015 Aug 27;162(5):1113-26. doi: 10.1016/j.cell.2015.08.007
Matsumoto N*, Sato K*, Nishimasu H*, Namba Y, Miyakubi K, Dohmae N, Ishitani R, Siomi H, Siomi MC & Nureki O (*equal contribution). Crystal structure and activity of the endoribonuclease domain of the piRNA pathway factor Maelstrom. Cell Reports 11, 1-10, 2015
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2015 Jan 29;517(7536):583-8. doi: 10.1038/nature14136. Epub 2014 Dec 10
Morita J, Kato K, Mihara E, Ishitani R, Takagi J, Nishimasu H, Aoki J, Nureki O. Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6. Acta Crystallogr F Struct Biol Commun. 2014 Jun;70(Pt 6):794-9. doi: 10.1107/S2053230X14008929. Epub 2014 May 24.
Yamagata K, Goto Y, Nishimasu H, Morimoto J, Ishitani R, Dohmae N, Takeda N, Nagai R, Komuro I, Suga H, Nureki O. Structural basis for potent inhibition of SIRT2 deacetylase by a macrocyclic peptide inducing dynamic structural change. Structure. 2014 Feb 4;22(2):345-52. doi: 10.1016/j.str.2013.12.001. Epub 2014 Jan 2.
Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O. Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13
Nishimasu, H.*, Ishizu, H.*, Saito, K., Fukuhara, S., Kamatani, M. K. , Bonnefond, L., Matsumoto, N., Nishizawa, T., Nakanaga, K., Aoki, J., Ishitani, R., Siomi, H., Siomi, M. C. & Nureki, O. (*equal contribution)
Structure and function of Zucchini endoribonuclease in piRNA biogenesis.
Nature 491, 284-287 (2012)
Tokunaga, F.*, Nishimasu, H.*, Ishitani, R., Goto, E., Noguchi, T., Mio, K., Kamei, K., Ma, A., Iwai, K. & Nureki, O. (*equal contribution)
Specific recognition of linear polyubiquitin by A20 zinc finger 7 is involved in NF-κB regulation.
EMBO J 31, 3856-3870 (2012)
Kato, K.*, Nishimasu, H.*, Okudaira, S., Mihara, E., Ishitani, R., Takagi, J., Aoki, J. & Nureki, O. (*equal contribution)
Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling.
Proc Natl Acad Sci USA 109, 16876-16881 (2012)
Fushinobu, S.*, Nishimasu, H.*, Hattori, D., Song, H. J. & Wakagi, T. (*equal contribution)
Structural basis for the bifunctionality of fructose-1,6-bisphosphate aldolase/phosphatase.
Nature 478, 538-541 (2011)
Nishimasu, H., Okudaira, S., Hama, K., Mihara, E., Dohmae, N., Inoue, A., Ishitani, R., Takagi, J., Aoki, J. & Nureki, O.
Crystal structure of autotaxin and insight into GPCR activation by lipid mediators.
Nat Struct Mol Biol 18, 205-212 (2011)
Nishimasu, H., Ishitani, R., Yamashita, K., Iwashita, C., Hirata, A., Hori, H. & Nureki, O.
Atomic structure of a folate/FAD-dependent tRNA T54 methyltransferase.
Proc Natl Acad Sci USA 106, 8180-8185 (2009)
Nishimasu, H., Fushinobu, S., Shoun, H. & Wakagi, T.
Crystal structures of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii.
J Biol Chem 282, 9923-9931 (2007)
Nishimasu, H., Fushinobu, S., Shoun, H. & Wakagi, T.
Identification and characterization of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii.
J Bacteriol 188, 2014-2019 (2006)
Nishimasu, H.*, Fushinobu, S.*, Shoun, H. & Wakagi, T. (*equal contribution)
The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic arhaea.
Structure 12, 949-959 (2004)
