塚崎 智也(ツカザキ トモヤ)

塚崎 智也
学位
理学博士
所属・職位
奈良先端科学技術大学院大学 バイオサイエンス研究科 准教授
URL
http://bsw3.naist.jp/courses/courses309.html
研究分野
構造生命科学

主要論文

  1. Furukawa A, Nakayama S, Yoshikaie K, Tanaka Y, Tsukazaki T.
    Remote coupled drastic β-barrel-sheet transition of the protein translocation motor.
    Structure. 2018 Jan 26. pii: S0969-2126(18)30002-9. doi: 10.1016/j.str.2018.01.002. [Epub ahead of print]
  2. Daimon Y, Iwama-Masui C, Tanaka Y, Shiota T, Suzuki T, Miyazaki R, Sakurada H, Lithgow T, Dohmae N, Mori H, Tsukazaki T, Narita S, Akiyama Y.
    The TPR domain of BepA is required for productive interaction with substrate proteins and the β-barrel assembly machinery complex.
    Mol Microbiol. 2017 Dec;106(5):760-776. doi: 10.1111/mmi.13844. Epub 2017 Oct 17.
  3. Tanaka Y, Iwaki S, Tsukazaki T.
    Crystal structure of a plant multidrug and toxic compound extrusion family protein
    Structure. 2017 Sep 5;25(9):1455-1460.e2.
  4. Sugano Y, Furukawa A, Nureki O, Tanaka Y, Tsukazaki T.
    SecY-SecA fusion protein retains the ability to mediate protein transport.
    PLoS One. 2017 Aug 18;12(8):e0183434.
  5. Furukawa A, Yoshikaie K, Mori T, Mori H, Morimoto YV, Sugano Y, Iwaki S, Minamino T, Sugita Y, Tanaka Y, Tsukazaki T.
    Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF.
    Cell Rep. 2017 May 2;19(5):895-901. doi: 10.1016/j.celrep.2017.04.030
  6. Tanaka Y, Sugano Y, Takemoto M, Mori T, Furukawa A, Kusakizako T, Kumazaki K, Kashima A, Ishitani R, Sugita Y, Nureki O, Tsukazaki T.:Crystal Structures of SecYEG in Lipidic Cubic Phase Elucidate a Precise Resting and a Peptide-Bound State. Cell Rep. 2015 Nov 24;13(8):1561-8. doi: 10.1016/j.celrep.2015.10.025. Epub 2015 Nov 12.
  7. Kumazaki K, Chiba S, Takemoto M, Furukawa A, Nishiyama K, Sugano Y, Mori T, Dohmae N, Hirata K, Nakada-Nakura Y, Maturana AD, Tanaka Y, Mori H, Sugita Y, Arisaka F, Ito K, Ishitani R, Tsukazaki T, Nureki O. Structural basis of Sec-independent membrane protein insertion by YidC. Nature. 2014 May 22;509(7501):516-20. doi: 10.1038/nature13167. Epub 2014 Apr 16.
  8. Kumazaki K, Tsukazaki T, Nishizawa T, Tanaka Y, Kato HE, Nakada-Nakura Y, Hirata K, Mori Y, Suga H, Dohmae N, Ishitani R, Nureki O. Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans. Acta Crystallogr F Struct Biol Commun. 2014 Aug;70(Pt 8):1056-60. doi: 10.1107/S2053230X14012540. Epub 2014 Jul 23.
  9. Kumazaki K, Kishimoto T, Furukawa A, Mori H, Tanaka Y, Dohmae N, Ishitani R, Tsukazaki T, Nureki O. Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase. Sci Rep. 2014 Dec 3;4:7299. doi: 10.1038/srep07299.
  10. Kaoru Kumazaki, Toshiki Kishimoto, Arata Furukawa, Hiroyuki Mori, Yoshiki Tanaka, Naoshi Dohmae, Ryuichiro Ishitani, Tomoya Tsukazaki, Osamu Nureki. Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase. Scientific Reports 4, 7299 (2014).
  11. Mio K, Tsukazaki T, Mori H, Kawata M, Moriya T, Sasaki Y, Ishitani R, Ito K, Nureki O, Sato C. Conformational variation of the translocon enhancing chaperone SecDF. J Struct Funct Genomics. 2014 Sep;15(3):107-15. doi: 10.1007/s10969-013-9168-4. Epub 2013 Dec 25.
  12. Tsukazaki, T., Mori, H, Echizen, Y., Ishitani, R., Fukai, S., Tanaka, T., Perederina A., Vassylyev, D.G., Kohno, T., Maturana, A.D., Ito, K., and Nureki, O. Structure and function of a membrane component SecDF that enhances protein export. Nature 474, 235-238 (2011).
  13. Tsukazaki, T. and Nureki, O. The mechanism of protein export enhancement by the SecDF membrane component. BIOPHYSICS, 7 129-133 (2011).
  14. Echizen, Y., Tsukazaki, T., Dohmae, N., Ishitani, R. and Nureki, O. Crystallization and preliminary X-ray diffraction of the first periplasmic domain of SecDF, a translocon-associated membrane protein, from Thermus thermophilus. Acta. Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 67, 1367-1370 (2011).
  15. Mori, T., Ishitani, R., Tsukazaki, T., Nureki, O. and Sugita, Y. Molecular mechanisms underlying the early stage of protein translocation through the Sec translocon. Biochemistry 49, 945-950 (2010).
  16. Tsukazaki, T., Mori, H., Fukai, S., Ishitani, R., Mori, T., Dohmae, N., Perederina, A., Sugita, Y., Vassylyev, D.G., Ito, K. and Nureki, O. Conformational transition of Sec machinery inferred from bacterial SecYE structures. Nature 455, 988-991 (2008).
  17. Tsukazaki, T., Mori, H., Fukai, S., Numata, T., Perederina, A., Adachi, H., Matsumura, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Sasaki, T., Vassylyev, D.G., Nureki, O. and Ito, K. Purification, crystallization and preliminary X-ray diffraction of SecDF, a translocon-associated membrane protein, from Thermus thermophilus. Acta. Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62, 376-380 (2006).
  18. Vassylyev, D.G., Mori, H., Vassylyeva, M.N., Tsukazaki, T., Kimura, Y., Tahirov, T.H. and Ito, K. Crystal structure of the translocation ATPase SecA from Thermus thermophilus reveals a parallel, head-to-head dimer. J. Mol. Biol. 364, 248-258 (2006).
  19. Vassylyeva, M.N., Mori, H., Tsukazaki, T., Yokoyama, S., Tahirov, T.H., Ito, K. and Vassylyev, D.G. Cloning, expression, purification, crystallization and initial crystallographic analysis of the preprotein translocation ATPase SecA from Thermus thermophilus. Acta. Crystallograph. Sect. F. Struct. Biol. Cryst. Commun. 62, 909-912 (2006).
  20. Mori, H., Tsukazaki, T., Masui, R., Kuramitsu, S., Yokoyama, S., Johnson, A.E., Kimura, Y., Akiyama, Y. and Ito, K., Fluorescence resonance energy transfer analysis of protein translocase. SecYE from Thermus thermophilus HB8 forms a constitutive oligomer in membranes. J. Biol. Chem. 278, 14257-14264 (2003).

論文

  1. Mio K, Tsukazaki T, Mori H, Kawata M, Moriya T, Sasaki Y, Ishitani R, Ito K, Nureki O, Sato C.:Conformational variation of the translocon enhancing chaperone SecDF. J Struct Funct Genomics. 2014 Sep;15(3):107-15. doi: 10.1007/s10969-013-9168-4. Epub 2013 Dec 25
  2. 塚崎智也,濡木理. タンパク質膜透過装置 膜タンパク質構造研究. 岩田想 編(化学同人) p44-52 (2013.10.15).
  3. 塚崎智也. 2つのSecモーター蛋白質による蛋白質膜透過のしくみ. 実験医学(増刊「構造生命科学で何がわかるのか,何ができるのか」), 32, 1571-1575 (2014).
  4. Doki, S., Kato, H.E., Solcan, N., Iwaki, M., Koyama, M., Hattori, M., Iwase, N., Tsukazak,i T., Sugita, Y., Kandori, H., Newstead, S., Ishitani, R. and Nureki, O. Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT. Proc Natl Acad Sci U S A 110, 11343-11348 (2013).
  5. Tanaka. Y., Hipolito, C.J., Maturana, A.D., Ito, K., Kuroda, T., Higuchi, T., Katoh, T., Kato, H.E., Hattori, M., Kumazaki, K., Tsukazaki, T., Ishitani, R., Suga, H. and Nureki, O. Structural basis for the drug extrusion mechanism by a MATE multidrug transporter. Nature 496, 247-251(2013).
  6. Kato, H.E., Zhang, F., Yizhar, O., Ramakrishnan, C., Nishizawa, T., Hirata, K., Ito, J., Aita, Y., Tsukazaki, T., Hayashi, S., Hegemann, P., Maturana, A.D., Ishitani, R., Deisseroth, K., and Nureki O. Crystal structure of the channelrhodopsin light-gated cation channel. Nature, 482, 369-374 (2012).
  7. Hattori, M., Iwase, N., Furuya, N., Tanaka, Y., Tsukazaki, T., Ishitani, R., Maguire, M.E., Ito, K. and Nureki, O. Mg2+-dependent gating of bacterial MgtE channel underlies Mg2+ homeostasis. EMBO J. 28, 3602-361 (2009).
  8. Hirota, S., Tsukazaki, T. and Yamauchi, O. Interactions of cytochrome c peroxidase with lysine peptides. Biochem. Biophys. Res. Commun. 268, 395-397 (2000)
  9. Hirota, S., Endo, M., Hayamizu, K., Tsukazaki, T., Takabe, T., Kohzuma, T. and Yamauchi, O. Interactions of Cytochrome c and Cytochrome f with Aspartic Acid Peptides. J. Am. Chem. Soc. 121, 849-855. (1999)
  10. Hirota, S., Endo, M., Tsukazaki, T., Takabe, T. and Yamauchi, O. Effects of charged peptides on electron transfer from [Fe(CN)6]4- to cytochrome c or plastocyanin. J. Biol. Inor. Chem. 3, 563-569 (1998).