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$B$3$N%_%K%3!<%9$G$O!"G[NsAjF1@-8!:w%W%m%0%i%`$G$"$k(JBLAST$B%U%!%_%j$K$D$$$F
$B"#(J $B2]Bj(J.1-1 $B%W%i%$%^!<$G$NA}I}NN0h$NFCDj(J
$B2<$K<($7$?%W%i%$%^!<$rMQ$$$k$3$H$GA}I}$G$-$k!"(JGenBank$B$KEPO?$5$l$F$$$k%R%H%2%N%`$NG[Ns$r(J BLAST$B$rMQ$$$FC5$7$J$5$$!#(J
Primer1: GTACCTTGATTTCGTATTC
Primer2: GACTCTACTACCTTTACCC
$B"#(J $B%R%s%H(J
forward$B$H(Jreverse$BN>J}$N%W%i%$%^!<$r(JBLAST$B$N(Jinput box$B$KE=$jIU$1$F$_$^$7$g$&!#$3$N;~$K:G=i$N(J
$B%W%i%$%^! $BC5:wHO0O$r%R%H%2%N%`$N$_$K9J$j9~$s$G$/$@$5$$!#(J $B7k2L$,F@$i$l$?$i!"$=$l$i$,F1$8G[Ns$KEv$?$C$F$$$k$3$H$r3NG'$7$F$/$@$5$$!#$^$?$3$l$i$N%R%C%H$O(J
DNA$B$NAjJd:?$K$"$?$C$F$$$J$1$l$P$$$1$^$;$s(J $B$$$/$D$N%R%C%H$,F@$i$l$^$7$?$+!)(J
$B"#(J $B2]Bj(J.1-2 $B0l1v4pB?7?(J(SNPs)$B$NFCDj(J
Hermankova,M$B$i$O!";R6!$HBg?M$,J;MQNEK!$G
BLAST $BF~NOG[Ns(J
$B"#(J $B2]Bj(J.2-1 $B%?%s%Q%/
$B2<$K<($5$l$?%"%_%N;@G[Ns$NAG@-$rD4$Y$F$/$@$5$$!#$3$NG[Ns$O(J
$B%7%g%&%8%g%&%P%($N(J5$B%(%/%=%s$rK]Lu$7$F:n@.$5$l$?$b$N$G$9!#(J
$B$3$N%?%s%Q%/ BLAST $BF~NOG[Ns(J
$B"#(J $B2]Bj(J.2-2 $B%?%s%Q%/
$B2<$K<($5$l$?%"%_%N;@G[Ns$NAG@-$rD4$Y$F$/$@$5$$!#$3$NG[Ns$O(J
$B%7%g%&%8%g%&%P%($N(J4$B%(%/%=%s$rK]Lu$7$F:n@.$5$l$?$b$N$G$9!#(J
$B$3$N%?%s%Q%/ BLAST $BF~NOG[Ns(J
$B"#(J $B2]Bj(J.3-1 $B%U%l!<%`%7%U%H0LCV$NFCDj(J
$B2<$K<($5$l$?3K;@G[Ns$N$I$3$K%U%l!<%`%7%U%H$,F~$C$F$$$^$9$+!)(J
BLAST $BF~NOG[Ns(J
$B"#(J $B2]Bj(J.3-2 $B%U%l!<%`%7%U%H0LCV$NFCDj(J
$B2<$K<($5$l$?3K;@G[Ns$N$I$3$K%U%l!<%`%7%U%H$,F~$C$F$$$^$9$+!)(J
BLAST $BF~NOG[Ns(J
$B"#(J $B2]Bj(J.4-1 Einstein$B$O(JGenBank$B$K$$$k$N!)(J
$B$$$C$?$$$$$/$D$N(J'einstein'$B$,(JGenBank$B$N%"%_%N;@G[Ns$K$O4^$^$l$F$$$k$N$G$7$g$&$+!)(J "$BC;$$$[$H$s$I40A4$K%^%C%A$9$kG[Ns$NC5:w(J"$BMQ$KMQ0U$5$l$?FCJL$J%Z!<%8$K=i4|@_Dj$5$l$F$$$k(J $B%Q%i%a!<%?$KCmL\$7$F$/$@$5$$!#FC$K(Jwordsize, expect, filter$B%*%W%7%g%s(J, $BHf3S%^%H%j%/%9(J $B$KCmL\$7$F$/$@$5$$!#(J
$B!J0JA0$N(Jblast$B$K$O(J"Search for short, nearly exact matches"$B$H$$$&FCJL$KMQ0U$5$l$?(J $B%5!<%S%9$,$"$C$?$N$G$9$,!"8=:_$O$"$j$^$;$s!#!K(J
$B"#(J $B2]Bj(J.4-2 Valentine$B$O(JGenBank$B$K$"$k$N!)(J
$B$$$C$?$$$$$/$D$N(J'valentein'$B$,(JGenBank$B$N%"%_%N;@G[Ns$K$O4^$^$l$F$$$k$N$G$7$g$&$+!)(J "$BC;$$$[$H$s$I40A4$K%^%C%A$9$kG[Ns$NC5:w(J"$BMQ$KMQ0U$5$l$?FCJL$J%Z!<%8$K=i4|@_Dj$5$l$F$$$k(J $B%Q%i%a!<%?$KCmL\$7$F$/$@$5$$!#FC$K(Jwordsize, expect, filter$B%*%W%7%g%s(J, $BHf3S%^%H%j%/%9(J $B$KCmL\$7$F$/$@$5$$!#(J
$B!J0JA0$N(Jblast$B$K$O(J"Search for short, nearly exact matches"$B$H$$$&FCJL$KMQ0U$5$l$?(J $B%5!<%S%9$,$"$C$?$N$G$9$,!"8=:_$O$"$j$^$;$s!#!K(J
$B"#(J $B2]Bj(J.5-1 Hoxb$B%[%b%m%0$NC5:w(J
$B%^%&%9(JHoxb$B$K4X$9$k(JmRNA(NM_008268), $B%"%_%N;@(JRefseq$BG[Ns(J(NP_032294)$B$rMQ$$$F(J reference genome $B$KBP$9$k(Jblast$BC5:w$G2?8D$N%[%b%m%0$,%^%&%9$G8+$D$+$k$+$rD4$Y$F$_$F$/$@$5$$!#(J
$B"#(J $B2]Bj(J.5-2 $B%W%m%H%+%I%X%j%s%[%b%m%0$NC5:w(J
$B%^%&%9%W%m%H%+%I%X%j%s$K4X$9$k(JmRNA(AY013770), $B%"%_%N;@G[Ns(J(AAK26059)$B$rMQ$$$F(J reference genome $B$KBP$9$k(Jblast$BC5:w$G2?8D$N%[%b%m%0$,%^%&%9$G8+$D$+$k$+$rD4$Y$F$_$F$/$@$5$$!#(J
$B"#(J $B2]Bj(J.6-1 Exon$B0LCV$NC5:w(J
$B2<$K<($7$?G[Ns$O(JGenBank$B$N(JHTG$B$KEPO?$5$l$?(JWRN Werener$B%7%s%I%m!<%`0dEA;R$N0lIt$r4^$s$@$b$N$G$9!#(J $B$3$N0dEA;R$O(J35$B8D$N(JExon$B$+$i$J$C$F$*$j!">e$K<($7$?$h$&$J(JcDNA$B9=B$$r$H$C$F$$$^$9!#(JBLAST2seq$B$rMQ$$$F(J WRN$B0dEA;R$N(JcDNA$BG[Ns$H$3$N(JHTG$B$KEPO?$5$l$?G[Ns$H$rHf$Y$k$3$H$G!"$3$N(JHTG$B$NG[Ns$,$I$N(JExon$B$N(J $B$b$N$+$rD4$Y$F$/$@$5$$!#(JSubject$BG[Ns$N8~$-$KCm0U$7$F$/$@$5$$!#(J
BLAST $BF~NOG[Ns(J
$B"#(J $B2]Bj(J.6-2 $B7gMn$7$?G[Ns$O!)(J
$B2<$K<($5$l$?G[Ns$r(JSequence1, Sequence2$B$NN>J}$KF~$l$F(JBLAST2sequecne$B$r BLAST $BF~NOG[Ns(J
NCBI$B$N%H%C%W%Z!<%8>eIt$+$i(JBLAST$B$rA*Br$7!"(JBLAST$B%a%K%e!<$N%Z!<%8$K0\F0$7$^$9!#(J
$B$=$3$+$i$^$:(Jblsatn($B3K;@Ld$$9g$o$;G[Ns(J vs $B3K;@%G!<%?%Y!<%9(J)($B@V;M3Q$G0O$^$l$?2U=j(J)$B$rA*Br$7$^$9!#(J
$B>eIt$N%F%-%9%H%\%C%/%9$KC5:w$7$?$$G[Ns(J($BFsK\$N%W%i%$%^!<$N4V$K(JN$B$r(J30$BJ8;zF~$l$?$b$N(J)$B$r(J
$BF~NO$7-!!"@8J*
$B$9$k$H2
$B?^$+$i(J2$BK\$NG[Ns$KBP$7$F%R%C%H$,$"$k$3$H$,3NG'$G$-$^$9(J($B@V;M3Q(J)$B!#(J
$B$3$l$i$N%R%C%H$O$$$:$l$b(J17$BHV@w?'BN$N$b$N$G$9$,!"2<$N(JNW$B$G;O$^$k%(%s%H%j$O(J
$B9q:]%3%s%=!<%7%"%`$K$h$k(Jreference$B%"%;%s%V%k$H$O0[$J$C$?8D?M$N%2%N%`$r:FFI$7$?(J
$B$b$N$G$"$j!"
$B7k2L2hLL$r2<$K%9%/%m!<%k$7$F$=$l$>$l$N7k2L$N%"%i%$%a%s%H$r8+$k$H!"(J
$B%W%i%$%^!<$N(Jforward/reverse$BAPJ}$NItJ,$,(JPlus$B$N(Jstrand$B$H(JMinus$B$N(Jstrand$B$K%R%C%H$7$F$$$k$3$H$,(J
$B3NG'$G$-$^$9!#!J(Jforward$B$N%^%C%T%s%0>pJsItJ,$,@D;M3Q$G0O$^$l$?2U=j!"(Jreverse$B$N%^%C%T%s%0>pJs$,(J
$B@V;M3Q$G0O$^$l$?2U=j!K(J
$B$3$l$K$h$j!"(Jforward/reverse$B$N%W%i%$%^!<$,5U8~$-$K@5$7$/%^%C%T%s%0$5$l$F$$$k$3$H$,3NG'$G$-$^$9!#(J
$B$3$N%W%i%$%^!<$K$h$jA}I}$5$l$kNN0h$O!"(J17$BHV@w?'BN$N(J5,001,573bp$B!A(J5,001,796bp$B$K$J$j$^$9!#(J
$BF1MM$K(Jblastn$B$NG[NsEjF~2hLL$K$*$$$F2]Bj$K<($5$l$?G[Ns$rF~NO$7$^$9!#(J
$B$?$@$7!":#2s$O%"%i%$%a%s%H7k2L$r8+$?$$$N$G!"=PNO$N(JAlignment View$B%*%W%7%g%s$N2U=j$r(J
flat-query anchored with identities$B$KJQ99$7$F(JBLAST$B$r
$B>eIt$N%F%-%9%H%\%C%/%9$KC5:w$7$?$$G[Ns$r(J
$BF~NO$7-!!"%G!<%?%Y!<%9$r(Jnr/nt$B$K$7-"!"8!:w%(%s%8%s$r(Jblast$B$K$7$?8e-#!"(J
BLAST$B%\%?%s$r%/%j%C%/$7$F-$C5:w$r3+;O$7$^$9!#(J $B$9$k$H!"?^$K<($5$l$?$h$&$K(JHIV-1$B$X$NB?$/$N%R%C%H$,G'$a$i$l$^$9!#(J
blast$B8!:w7k2L$NI=<(%U%)!<%^%C%HJQ99(J
$BB3$$$F!"=PNO%U%)!<%^%C%H$rJQ99$7$^$9!#(J
$B7k2L2hLL$N>eIt$N(J"Formatting options"$B$r%/%j%C%/$7$F$/$@$5$$!J?^Cf@V;M3Q!K!#(J
$B$9$k$H0J2<$N$h$&$K=PNO%U%)!<%^%C%H$r;XDj$9$k2hLL$,I=<($5$l$^$9$N$G!"(JDisplay$B%W%k%@%&%s%a%K%e!<(J $B$+$i(J"Flat query-anchored with dots for identities"$B$rA*Br$7!"1&>e$N(J"Reformat"$B%\%?%s$r%/%j%C%/$7$F(J $B:FIA2h$7$F$/$@$5$$!#(J
$B2hLL$r2<$K%9%/%m!<%k$H!"(Jblast$B$N7k2L$,%^%k%A%W%k%"%i%$%a%s%HMM$K<($5$l$F$$$^$9!#(J Query$BG[Ns$HF1$8G[Ns$O(J"."$B$G!"0c$&$H$3$m$@$1$,(JACGT$B$G<($5$l$F$$$^$9!#(J
$B?^$+$i(J6$B1v4pL\$K$O(JA/G$B$NB?7?$,$"$k$3$H$,$o$+$j$^$9!#(J
$B$^$:!"(JBLAST$B$N%H%C%W%Z!<%8$+$i(J"protein blast"$B$rA*Br$7$^$9!#(J
$B$7$P$i$/$7$FF@$i$l$k7k2L$r8+$k$H!"$=$N>e0L$O$$$:$l$b(JAspartate aminotransferase$B$G!"(J
Pongo, Mouse, Rat, Pig$B$J$I$5$^$6$^@8J*$N%?%s%Q%/ $B%R%C%H$7$?G[Ns$N>\:Y$J>pJs$O7k2L$N1&2#$K(J"G"$B%^!<%/$N$D$$$F$$$k2U=j$r%/%j%C%/$9$k$3$H$G(JEntrez Gene$B$N%Z!<%8(J
$B$X0\F0$9$k$3$H$G3NG'$G$-$^$9!#(J $B$3$l$i$N>pJs$+$i(J
$BLd$$9g$o$;G[Ns$O(J $B%7%g%&%8%g%&%P%($N(JAspartate aminotransferase$B$G$"$k$3$H$,N`?d$5$l$^$9!#(J
$BF1MM$K(Jblastp$B$N%Z!<%8$K2]Bj$N(J
$BLd$$9g$o$;G[Ns$rF~NO-!!"%G!<%?%Y!<%9$+$i(Jswissprot$B$rA*Br$7-"!"(J
$B8!:w%(%s%8%s$H$7$F(Jblastp$B$rA*Br8e-#!"(Jblast$B%\%?%s$r2!$7$F-$8!:w$r
$B$7$P$i$/$7$FF@$i$l$k7k2L$r8+$k$H!"$=$N>e0L$O$$$:$l$b(JPhosphoglucomutase $B$G(J $B!"(J
$B%7%g%&%8%g%&%P%(!"(JHuman$B$J$I$5$^$6$^@8J*$N%?%s%Q%/
$BM?$($i$l$?3K;@G[Ns$N$I$3$K%U%l!<%`%7%U%H$,F~$C$F$$$k$+$rD4$Y$k$?$a$K(Jblastx$B$rMQ$$$^$9!#(J BLAST$B%a%K%e!<$N%Z!<%8$+$i(Jblastx($B3K;@Ld$$9g$o$;G[Ns(J vs $B%"%_%N;@%G!<%?%Y!<%9(J)$B$rA*Br$7$^$9!#(J
$BLd$$9g$o$;G[Ns$H$7$FM?$($i$l$?G[Ns$rF~NO$7-!!"(J
$B$=$N$[$+$O%G%U%)%k%H$N$^$^$G(JBLAT$B%\%?%s$r%/%j%C%/$9$k-"$3$H$G(J
$B%[%b%m%8!<8!:w$r
$B7k2L$r8+$k$HLd$$9g$o$;G[Ns$O(Jenvelope glycoprotein$B$G$"$k$3$H$,?d;!$5$l!"2<$K%9%/%m!<%k$7$F(J
$B$=$N%"%i%$%a%s%H$r8+$k$H%"%i%$%a%s%H$,Fs$D$KJ,CG$5$l%U%l!<%`$,$:$l$F$$$k$3$H$,$o$+$j$^$9!#(J
$BFs$DL\$N%"%i%$%a%s%H$,(J+2$B$N%U%l!<%`$KBP$9$k$b$N$G!"(J
$BLd$$9g$o$;G[Ns$N(J268bp$B$G=*$o$C$F$*$j!J@V$/0O$^$l$?2U=j$r;2>H!K!"(J
$B0l$DL\$N%"%i%$%a%s%H$,(J+1$B$N%U%l!<%`$KBP$9$k$b$N$G!"Ld$$9g$o$;G[Ns$N(J268bp$B$+$i;O$^$C$F$$$k(J
$B!J@D$/0O$^$l$?2U=j$r;2>H!K$3$H$+$i!"$=$NJ,CG2U=j$OLd$$9g$o$;G[Ns$N(J268$B1v4pL\IU6a$G$"$k$3$H$,(J
$B3NG'$G$-$^$9!#(J
blastx($B3K;@Ld$$9g$o$;G[Ns(J vs $B%"%_%N;@%G!<%?%Y!<%9(J)$B$N%Z!<%8$+$i!"(J
$BLd$$9g$o$;G[Ns$H$7$FM?$($i$l$?G[Ns$rF~NO$7-!!"(J
$B$=$N$[$+$O%G%U%)%k%H$N$^$^$G(JBLAT$B%\%?%s$r%/%j%C%/$9$k-"$3$H$G(J
$B%[%b%m%8!<8!:w$r
$B7k2L$r8+$k$HLd$$9g$o$;G[Ns$O(Jenvelope glycoprotein$B$G$"$k$3$H$,?d;!$5$l!"2<$K%9%/%m!<%k$7$F(J
$B$=$N%"%i%$%a%s%H$r8+$k$H%"%i%$%a%s%H$,Fs$D$KJ,CG$5$l%U%l!<%`$,$:$l$F$$$k$3$H$,$o$+$j$^$9!#(J
$BFs$DL\$N%"%i%$%a%s%H$,(J+1$B$N%U%l!<%`$KBP$9$k$b$N$G!"(J
$BLd$$9g$o$;G[Ns$N(J564bp$B$G=*$o$C$F$*$j!J@V$/0O$^$l$?2U=j$r;2>H!K!"(J
$B0l$DL\$N%"%i%$%a%s%H$,(J+21$B$N%U%l!<%`$KBP$9$k$b$N$G!"Ld$$9g$o$;G[Ns$N(J566bp$B$+$i;O$^$C$F$$$k(J
$B!J@D$/0O$^$l$?2U=j$r;2>H!K$3$H$+$i!"$=$NJ,CG2U=j$OLd$$9g$o$;G[Ns$N(J565$B1v4pL\IU6a$G$"$k$3$H$,(J
$B3NG'$G$-$^$9!#(J
$B0JA0$N(Jblast$B$K$O(J"Search for short, nearly exact matches"$B$H$$$&FCJL$KMQ0U$5$l$?(J $B%5!<%S%9$,$"$C$?$N$G$9$,!"8=:_$O$"$j$^$;$s!#=>$C$F!"DL>o$N(Jblast$BC5:w$N%Q%i%a!<%?$r(J $BJQ99$9$k$3$H$G!"F1MM$N7k2L$,F@$i$l$k$h$&$KBP1~$7$?2sEz$r>R2p$7$^$9!#(J
BLAST$B%a%K%e!<$N%Z!<%8$+$i(Jblastp$B$rA*Br$7!"Ld$$9g$o$;G[Ns$H$7$F(J"einstein"$B$HF~NO$7-!!"(J $B%G!<%?%Y!<%9$H$7$F(J"nr"$B$rA*Br$7$F$/$@$5$$-"!#(J
$B\:Y$J%Q%i%a!<%?@_Dj2hLL$rI=<($5$;$F$/$@$5$$!#(J
$B%G!<%?%Y!<%9$X$N%R%C%H$,B?$$$3$H$rA[Dj$7$F!"(J"Max target sequences"$B!J2?K\$^$G$N%R%C%H$r(J
$BI=<($9$k$+!K$r(J1000$B$K-!!"(J"Word size"$B$r$h$jC;$$(J2$B$K-"!"(J"Matrix"$B$r(J
PAM30$B$H$$$&C;$$Ld$$9g$o$;G[Ns8~$-$N$b$N$K-#JQ99$7$^$9!#(J
$B$=$N8e!"(Jblast$B%\%?%s$r2!$7$F%[%b%m%8!<8!:w$r $B$7$P$i$/BT$D$H7k2L$,I=<($5$l$^$9!#6K$a$FB?$/$N%R%C%H$,G'$a$i$l$^$9!#%H%C%W%R%C%H$N(J
$B%"%i%$%a%s%H$r8+$F$_$^$7$g$&!#(J Score$BItJ,$K=q$+$l$??t;z$r%/%j%C%/$7$F$_$F$/$@$5$$!#3:Ev$9$k%"%i%$%a%s%H$K%8%c%s%W$7$^$9!#(J $B%"%i%$%a%s%H$h$jL@$i$+$J$h$&$K!"40A40lCW$9$k$b$N$O(J1$BK\$bF@$i$J$+$C$?$3$H$,$o$+$j$^$9!#(J $B$A$J$_$K8=:_$N(Jblastp$B8!:w$G$O!"7k2L$N:G>eIt$r8+$k$HJ,$+$k$h$&$K!"Ld$$9g$o$;G[Ns$,(J
$BC;$$>l9g$K$O<+F0E*$K$=$l$K9g$o$;$?%Q%i%a!<%?$,@_Dj$5$l$F%[%b%m%8!<8!:w$,
$B0JA0$N(Jblast$B$K$O(J"Search for short, nearly exact matches"$B$H$$$&FCJL$KMQ0U$5$l$?(J $B%5!<%S%9$,$"$C$?$N$G$9$,!"8=:_$O$"$j$^$;$s!#=>$C$F!"DL>o$N(Jblast$BC5:w$N%Q%i%a!<%?$r(J $BJQ99$9$k$3$H$G!"F1MM$N7k2L$,F@$i$l$k$h$&$KBP1~$7$?2sEz$r>R2p$7$^$9!#(J
4-1$B$HF1MM$NA`:n$r(J'valentein'$BG[Ns$KJQ$($F $B\:Y$J%Q%i%a!<%?@_Dj2hLL$rI=<($5$;$F$/$@$5$$!#(J $B%G!<%?%Y!<%9$X$N%R%C%H$,B?$$$3$H$rA[Dj$7$F!"(J"Max target sequences"$B!J2?K\$^$G$N%R%C%H$r(J
$BI=<($9$k$+!K$r(J1000$B$K-!!"(J"Word size"$B$r$h$jC;$$(J2$B$K-"!"(J"Matrix"$B$r(J
PAM30$B$H$$$&C;$$Ld$$9g$o$;G[Ns8~$-$N$b$N$K-#JQ99$7$^$9!#(J
$B$=$N8e!"(Jblast$B%\%?%s$r2!$7$F%[%b%m%8!<8!:w$r $B$7$P$i$/BT$D$H7k2L$,I=<($5$l$^$9!#6K$a$FB?$/$N%R%C%H$,G'$a$i$l$^$9!#%H%C%W%R%C%H$N(J
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BLAST$B%a%K%e!<$N%Z!<%8$+$i(J"BLAST Assembled Genomes"$B"M(J"Mouse"$B$rA*Br$7$^$9!#(J
query$B$N%F%-%9%H%\%C%/%9$K(J"NM_008268"$B$HF~NO$7-!!"(J
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$BLd$$9g$o$;G[Ns$H$7$F $B3K;@(J vs $B3K;@$N8!:w(J
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$B$K0lHV9b$$%9%3%"$GA4D9$K$o$?$C$F%R%C%H$7!"$=$l0J30$KA4It$G(J11$B2U=j!"(JE-value:1.0e-10$B0J2<(J
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$BLd$$9g$o$;G[Ns$H$7$F%"%_%N;@G[Ns$N%"%/%;%C%7%g%sHV9f$G$"$k(J"NP_032294"$B$rF~NO$7-!!"(J
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$B8!:w$,2DG=$G$9!#$3$N:]%G!<%?%Y!<%9B&$O(J6$B%U%l!<%`$9$Y$F$r%"%_%N;@$KJQ49$7!"%"%_%N;@(J vs $B%"%_%N;@$N(J
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$BHf3S$9$k$[$&$,46EY$,9b$$$3$H$,$o$+$j$^$9!#(JmRNA$B$H$NHf3S$G$O0lIt$7$+%R%C%H$,G'$a$i$l$J$+$C$?(J2$B7o(J
(6,15$BHV@w?'BN$X$N%R%C%H(J)$B$b$[$\A4D9$K$o$?$C$FN`;w$7$F$$$k$3$H$,3NG'$G$-$^$9!#2]Bj$K$"$C$?(J
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"Begin Search"$B%\%?%s$r2!$9$3$H$G-$!"%[%b%m%8!<8!:w$r AY013770$B$rF~NO$H$7$?Nc$G$O!"$3$NG[Ns$,B8:_$9$k(J18$BHV@w?'BN$X$N40A4$J%R%C%H$,(J1$B7o8+$i$l$k$N$_$G(J
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Database$B$O(J"genome (reference only)"$B$r-"!"(JProgram$B$O(J"tblastn"$B$rA*Br$7$F-#(J
"Begin Search"$B%\%?%s$r2!$9$3$H$G-$!"%[%b%m%8!<8!:w$r $B3K;@F1;N$NHf3S$G$O8+$D$1$i$l$J$+$C$?%[%b%m%0$,?tB?$/G'$a$i$l$^$9!#Nc$($PogCM$r(JE-value$B!'(J1.0e-10$B$H$9$k$H(J
13$B8D$H$J$j$^$9(J(6e-159$B!A(J1e-31)$B!#(J
BLAST$B%a%K%e!<$N%Z!<%8$+$i(JAlign two sequences using BLAST(bl2seq)$B$rA*Br$7$^$9!J@V;M3Q!K!#(J
$BB3$$$FG[NsEjF~2hLL$G(JSequence1, 2$B$K$=$l$>$l2]Bj$G<($5$l$?G[Ns$rF~NO$7!"$=$N$[$+$O%G%U%)%k%H$N%Q%i%a!<%?$N$^$^$G(J
$B%[%b%m%8!<8!:w$r $B7k2L$N2hLL$G$O$^$:>eIt$K$3$NFsK\$NG[Ns$N$I$NNN0h$,N`;w$7$?$+$,LO<0?^$*$h$S%I%C%H%^%H%j%/%9(J
($B%O!<%W%m%C%H(J)$B$K;w$?7A$G<($5$l$^$9(J($B?^Cf@V$G0O$C$?ItJ,(J)$B!#$=$N2<$K%"%i%$%a%s%H$N>\:Y$,<($5$l$F$$$^$9!#(J
$B:#2sMQ$$$?G[Ns$G$O0lK\L\$NG[Ns$N(J116bp$B$+$i(J233bp$BL\$,FsK\L\$NG[Ns$N(J954bp$BL\$+$i(J1071bp$BL\$K%R%C%H$7$F$$$k(J
$B$3$H$,$o$+$j$^$9!#(J
$B0lK\L\$NG[Ns$,(JHTG($B%2%N%`CGJR$N0lIt(J)$B$G!"FsK\L\$NG[Ns$,(JcDNA$B$G$"$k$3$H$r9M$($k$H!"0lK\L\$NG[Ns$N(J116-233bp$BL\(J
$BIU6a$,%(%/%=%s$G$=$NA08e$,%$%s%H%m%s$G$"$k$3$H!"$=$N%(%/%=%s$O(JcDNA$B>e$N(J954-1071bp$BL\IU6a$G$"$k$3$H$,(J
$B$o$+$j$^$9!#$3$l$r2]Bj$K$"$C$??^$K>H$i$79g$o$;$k$H$[$\%(%/%=%s(J8$B$KAjEv$7$^$9!#$h$C$F$3$N(JHTG$B$K$O%(%/%=%s(J8$B$,(J
$B4^$^$l$F$$$k$H3NG'$G$-$^$9!#(J
$BA4$/F1MM$K(JBLAST$B%a%K%e!<$N%Z!<%8$+$i(JAlign two sequences using BLAST(bl2seq)$B$rA*Br8e!"(J
Sequence1, 2$B$K$=$l$>$l2]Bj$G<($5$l$?G[Ns$rF~NO$7!"$=$N$[$+$O%G%U%)%k%H$N%Q%i%a!<%?$N$^$^$G(J
$B%[%b%m%8!<8!:w$r $B$9$k$H!"0J2<$N$h$&$KFs$D$KJ,CG$5$l$?7A$G7k2L$,F@$i$l$^$9!#N>
$B2<$K%9%/%m!<%k$7$F%"%i%$%a%s%H$r8+$k$H(J649bp$BL\$+$i(J752bp$BL\$+$i$,7g$1$F$$$k$h$&$G$9!#(J
$B$3$l$O%G%U%)%k%H$G%U%#%k%?%*%W%7%g%s$,(Jon$B$K$J$C$F$*$j!"$3$NNN0h$,(JLow Complexity$B$H(J
$BH=CG$5$l$?$3$H$K5/0x$9$k$H;W$o$l$^$9!#$=$3$G!"2hLL>eIt$N%*%W%7%g%s$G(JFilter$B$K$D$1$i$l$?(J
$B%A%'%C%/$r30$7$F:FEY(JAlign$B%\%?%s$r2!$7$F8!:w$r $B:#EY$OJ,CG$5$l$k$3$H$J$/A4D9$,%"%i%$%a%s%H$G$-$?$3$H$,3NG'$G$-$^$9!#(J
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