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’˜ŽÒE”­•\ŽÒHiroaki Ozaki, Masayasu Kuwahara and Hiroaki Sawai Š‘®F‘SˆõŒQ”n‘åŠw‘åŠw‰@HŠwŒ¤‹†‰È
•\‘èOligonucleotides Bearing a 5-substituted Pyrimidine Nucleoside: Their Synthesis, Properties, and Application
”­•\æFrontiers in Organic Chemistry, 1, (1), (pp63-78), Edited by Yoshihiro Hayakawa, Bentham Science Publishers @¨uê–发v
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’˜ŽÒE”­•\ŽÒJun-ichi Nagashima, Satoshi Minezaki, Satoshi Obika, Takeshi Imanishi, Masayasu Kuwahara and Hiroaki Sawai ¬”ä‰êæ¶E¡¼æ¶F‘åã‘åŠw‘åŠw‰@–òŠwŒ¤‹†‰È ‚»‚Ì‘¼FŒQ”n‘åŠw‘åŠw‰@HŠwŒ¤‹†‰È
•\‘èPolymerisation of a DNA strand using oligo-DNA template with modified bases, sugars and phosphates (oral)
”­•\æThe 5th International Symposium on Nucleic Acids Chemistry ”õlFOralC1Œ Nucleic Acids Res. Sym.Ser., 51, 55-56 (2008)
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’˜ŽÒE”­•\ŽÒ‰i“‡ˆêC•õèŒ«–¤CŒKŒ´³–õC”öèL–¾CàVˆäG–¾ Š‘®F‘SˆõŒQ”n‘åŠw‘åŠw‰@HŠwŒ¤‹†‰È
•\‘èDNA‚̎卽ƒŠƒ“Ž_•”ˆÊ‚̉ü•Ï‚ªƒ|ƒŠƒƒ‰[ƒ[”½‰ž‚É‹y‚Ú‚·‰e‹¿
”­•\æ ‘æ10‰ñ@¶–½‰»ŠwŒ¤‹†‰ïƒVƒ“ƒ|ƒWƒEƒ€
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’˜ŽÒE”­•\ŽÒHiroaki Sawai, Jun-ichi Nagashima, Masayasu Kuwahara, Rina Kitagata, Takehiro Tamura, Ikuo Matsui
•\‘èDifferences in substrate specificity of C(5)-substituted or C(5)-unsubstituted pyrimidine nucleotides by DNA polymerases from thermophilic bacteria, archaea, and phages.
”­•\æChem. Biodivers., 4, (9), 1979-1995 (2007).
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’˜ŽÒE”­•\ŽÒKazuomi Ohsawa, Toshiyuki Kasamatsu, Jun-ichi Nagashima, Kazuo Hanawa, Masayasu Kuwahara, Hiroaki Ozaki and Hiroaki Sawai
•\‘èArginine-modified DNA Aptamers That Show Enantioselective Recognition of the Dicarboxylic Acid Moiety of Glutamic Acid
”­•\æAnalytical Sciences, 2008, 24(1), 167-172.
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’˜ŽÒE”­•\ŽÒ•õèŒ«–¤E‰i“‡ˆêEú㌴³–õE”öèL–¾EàVˆäG–¾ Š‘®F‘SˆõŒQ”n‘åŠw‘åŠw‰@HŠwŒ¤‹†‰È
•\‘èDNAƒ|ƒŠƒƒ‰[ƒ[‚É‚æ‚éŽå½œŠiCü’’Œ^½‚Ì”z—ñ“ÇŽæ‚è
”­•\æ‘æ22‰ñ¶‘Ì‹@”\ŠÖ˜A‰»ŠwƒVƒ“ƒ|ƒWƒEƒ€ iŒû“ª1Œj
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’˜ŽÒE”­•\ŽÒMasayasu Kuwahara, Jun-ichi Nagashima, Hidetoshi Takeshima, Hiroaki Ozaki and Hiroaki Sawai Š‘®FGraduate School of Engineering, Gunma University
•\‘èSynthesis of acridonyl-dUTP analog and its substrate property during a polymerase reaction
”­•\æThe 2nd international workshop on approaches to single-cell analysis iƒ|ƒXƒ^[1Œj
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’˜ŽÒE”­•\ŽÒú㌴³–õ Š‘®FŒQ”n‘åŠw‘åŠw‰@HŠwŒ¤‹†‰È
•\‘èlHŠjŽ_•ªŽq‚̉”\«`ŠjŽ_ˆã–òEf’f–ò‚Ì‘n»‚ð‚ß‚´‚µ‚ā`
”­•\æ ‘æ22‰ñ@¶‘Ì‹@”\ŠÖ˜A‰»ŠwƒVƒ“ƒ|ƒWƒEƒ€uŽáŽèƒtƒH[ƒ‰ƒ€v
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’˜ŽÒE”­•\ŽÒ‰i“‡ˆêCú㌴³–õC”öèL–¾CàVˆäG–¾
•\‘èCüŠîŽ¿‚ð—p‚¢‚½ƒvƒ‰ƒCƒ}[L’·”½‰ž‚Ì‘¬“x˜_“I‰ðÍ
”­•\æ“ú–{‰»Šw‰ï@‘æ87‰ñt‹G”N‰ï
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’˜ŽÒE”­•\ŽÒ¯Ži“ÖŽmCú㌴³–õC”öèL–¾CàVˆäG–¾
•\‘è(R)-ƒTƒŠƒhƒ}ƒCƒh‚É“ÁˆÙ“I‚ÉŒ‹‡‚·‚éCüDNAƒAƒvƒ^ƒ}[‚Ì‘n»‚Æ‚»‚Ì‹@”\‰ðÍ
”­•\æ“ú–{‰»Šw‰ï@‘æ87‰ñt‹G”N‰ï
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’˜ŽÒE”­•\ŽÒMasayasu Kuwahara, Yoshiyuki Suto, Satoshi Minezaki, Rina Kitagata, Jun-ichi Nagashima and Hiroaki Sawai
•\‘èSubstrate property and incorporation accuracy of various dATP analogs during enzymatic polymerization using thermostable DNA polymerases.
”­•\æ Nucleic Acids Res. Sym.Ser., 50, 31-32 (2006) (Thirty-third Symposium on Nucleic Acids Chemistry)iŒû“ª‚PŒj
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’˜ŽÒE”­•\ŽÒXŠp—T•½EŠ}¼•qKEú㌴³–õE”öèL–¾EàVˆäG–¾ Š‘®F‘SˆõŒQ”n‘åŠwHŠw•”
•\‘èDNA‚Ì“ñŽŸ\‘¢‚ð”FŽ¯‚·‚éCüDNAƒAƒvƒ^ƒ}[‚ÌSPR‚É‚æ‚é‹@”\‰ðÍ
”­•\æ¶‘Ì‹@”\ŠÖ˜A‰»Šw•”‰ïEƒoƒCƒIƒeƒNƒmƒƒW[•”‰ïE¶–½‰»ŠwŒ¤‹†‰ï‡“¯ƒVƒ“ƒ|ƒWƒEƒ€(2006) iŒû“ª‚PŒj
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’˜ŽÒE”­•\ŽÒú㌴³–õ Š‘®FŒQ”n‘åŠwHŠw•”
•\‘èƒCƒ“ƒrƒgƒEƒZƒŒƒNƒVƒ‡ƒ“–@‚É‚æ‚é‹@”\«CüDNA•ªŽq‚Ì‘n»
”­•\æ “ú–{–òŠw‰ïE‘æ127”N‰ïiµ‘ҍu‰‰EŒû“ª‚PŒj
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’˜ŽÒE”­•\ŽÒAtsushi Shoji, Masayasu Kuwahara, Hiroaki Ozaki and Hiroaki Sawai
•\‘èA modified DNA aptamer that binds the (R)-isomer of a thalidomide derivative with high enantioselectivity.
”­•\æJournal of the American Chemical Society, 129, (5), 1456-1464 (2007)
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’˜ŽÒE”­•\ŽÒMasayasu Kuwahara, Yusuke Katouno, Jun-ichi Nagashima and Hiroaki Sawai
•\‘èScreening of catalytic modified DNA molecules from histidine-modified DNA library
”­•\æThe second international symposium on biomolecular chemistry
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’˜ŽÒE”­•\ŽÒMasayasu Kuwahara, Jun-ichi Nagashima, Masatoshi Hasegawa, Takehiro Tamura, Rina Kitagata, Kazuo Hanawa, Shin-ichi Hososhima, Toshiyuki Kasamatsu, Hiroaki Ozaki and Hiroaki Sawai
•\‘èSystematic characterization of 2f-deoxynucleoside- 5f-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA.
”­•\æNucleic acids research, 34, (19), 5383-5394 (2006)
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’˜ŽÒE”­•\ŽÒã‰“–ì—Y‰îCú㌴³–õCàVˆäG–¾
•\‘èRNA‚ð”z—ñ“ÁˆÙ“I‚ɐؒf‚·‚éƒqƒXƒ`ƒWƒ“CüDNAzyme‚Ì‘n»
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’˜ŽÒE”­•\ŽÒú㌴³–õCŠ}¼•qKC‘å‘ò˜abCàVˆäG–¾
•\‘èƒOƒ‹ƒ^ƒ~ƒ“Ž_‚É“ÁˆÙ“I‚ÉŒ‹‡‚·‚éƒAƒ‹ƒMƒjƒ“CüƒAƒvƒ^ƒ}[‚Ì‘n»
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’˜ŽÒE”­•\ŽÒú㌴³–õC’·’Jì‰ërC“c‘º‘sLC{“¡‰À”VCàVˆäG–¾
•\‘èŽíX‚̏CüƒkƒNƒŒƒIƒ`ƒh‚̍y‘f“IŽæ‚荞‚Ý‚É‚æ‚éDNAƒ‰ƒCƒuƒ‰ƒŠ‚Ì‘½—l‰»
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’˜ŽÒE”­•\ŽÒ¯Ži“ÖŽmCú㌴³–õCàVˆäG–¾
•\‘èƒTƒŠƒhƒ}ƒCƒhŒ‹‡CüDNAƒAƒvƒ^ƒ}[‚Ì‘I•Ê‚ÆSPR‚É‚æ‚é‹@”\•]‰¿
”­•\æ ‘æ8‰ñ@¶–½‰»ŠwŒ¤‹†‰ïƒVƒ“ƒ|ƒWƒEƒ€
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’˜ŽÒE”­•\ŽÒú㌴³–õC’·’Jì‰ërC–k•û—¢“ށC”öèL–¾CàVˆäG–¾
•\‘葽dCüDNAƒ‰ƒCƒuƒ‰ƒŠ‚ð—p‚¢‚½”­“WƒCƒ“ƒrƒgƒEƒZƒŒƒNƒVƒ‡ƒ“–@‚ÌŠJ”­
”­•\æ JSTESORSTƒWƒ‡ƒCƒ“ƒgƒVƒ“ƒ|ƒWƒEƒ€(4)
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’˜ŽÒE”­•\ŽÒMasayasu Kuwahara, Kazuo Hanawa, Kazuomi Ohsawa, Rina Kitagata, Hiroaki Ozaki and Hiroaki Sawai
•\‘èDirect PCR amplification of various modified DNAs having amino acids: convenient preparation of DNA libraries with high-potential activities for in vitro selection
”­•\æBioorganic & Medicinal Chemistry, 14, (8), 2518-26 (2006)
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’˜ŽÒE”­•\ŽÒMasayasu Kuwahara, Takehiro Tamura, Rina Kitagata, Hiroaki Sawai, Hiroaki Ozaki
•\‘èComparison study on PCR amplification of modified DNA by using various kinds of polymerase and modified nucleoside triphosphates.
”­•\æNucleic Acids Res. Sym.Ser., 49, 275-276 (2005) (The 4th International Symposium on Nucleic Acids Chemistry)
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’˜ŽÒE”­•\ŽÒMasayasu Kuwahara, Kazuomi Ohsawa, Toshiyuki Kasamatsu, Atsushi Shoji, Hiroaki Sawai, Hiroaki Ozaki
•\‘èScreening of a glutamic acid-binding aptamer from arginine-modified DNA library.
”­•\æNucleic Acids Res. Sym.Ser., 49, 81-82 (2005) (The 4th International Symposium on Nucleic Acids Chemistry)
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’˜ŽÒE”­•\ŽÒTatsuo Ohmichi, Masayasu Kuwahara, Naoko Sasaki, Masatoshi Hasegawa, Takahito Nishikata, Hiroaki Sawai and Naoki Sugimoto
•\‘èNucleic acid with guanidinium modification exhibits efficient cellular uptake.
”­•\æAngew. Chem. Int. Ed., 44, (41), 6682-6685.
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’˜ŽÒE”­•\ŽÒTsutomu Ohbayashi, Masayasu Kuwahara, Masatoshi Hasegawa, Toshiyuki Kasamatsu, Takehiro Tamura and Hiroaki Sawai
•\‘èExpansion of repertoire of modified DNAs prepared by PCR using KOD Dash DNA polymerase.
”­•\æOrg. Biomol. Chem., 3,(13), 2463-2468 (2005)
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’˜ŽÒE”­•\ŽÒMohammad M. Masud, Akiko N. Ozaki, Masayasu Kuwahara, Hiroaki Ozaki and Hiroaki Sawai
•\‘èModified DNA Bearing 5-(Methoxycarbonylmethyl)-2f-deoxyuridine: Preparation by PCR with Thermophilic DNA Polymerase and Post Synthetic Derivatization
”­•\æChemBioChem, 4, 584-588 (2003).
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’˜ŽÒE”­•\ŽÒMasayasu Kuwahara, Yumi Takahata, Atsushi Shoji, Akiko N. Ozaki, Hiroaki Ozaki and Hiroaki Sawai
•\‘èSubstrate@Properties of@C5-Substituted@Pyrimidine 2f-Deoxynucleoside 5f-Triphosphates for Thermostable DNA Polymerases during PCR
”­•\æBioorg. Med. Chem. Lett., 13, (21), 3735-3738 (2003).
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’˜ŽÒE”­•\ŽÒMasud M. Mohammad, Masayasu Kuwahara, Hiroaki Ozaki, Hiroaki Sawai
•\‘èSialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX
”­•\æBioorganic & Medicinal Chemistry (2004), 12(5), 1111-1120.
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’˜ŽÒE”­•\ŽÒú㌴³–õ
•\‘èPCR‚É‚æ‚éCüDNA‚ÌŒø—¦“I’²»–@‚ÆŽŽŒ±ŠÇ“à‘I‘ð–@‚ւ̉ž—p
”­•\æFIBERƒtƒH[ƒ‰ƒ€2004 (ŽåÃFb“ì‘åŠwæ’[¶–½HŠwŒ¤‹†Š)
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’˜ŽÒE”­•\ŽÒ‰i“‡ˆêCú㌴³–õCàVˆäG–¾
•\‘èCüDNA‚̍y‘f“I‡¬–@‚Æ‚»‚̉ž—p(4):ƒAƒ‹ƒh[ƒ‹”½‰ž‚ðG”}‚·‚éCüDNA‚Ì‘n»
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’˜ŽÒE”­•\ŽÒ¯Ži“ÖŽmCú㌴³–õC”öèL–¾CàVˆäG–¾
•\‘èCüDNA‚̍y‘f“I‡¬–@‚Æ‚»‚̉ž—p(3):ƒTƒŠƒhƒ}ƒCƒhŒ‹‡CüDNAƒAƒvƒ^ƒ}[‚Ì‘n»
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•\‘èCüDNA‚̍y‘f“I‡¬–@‚Æ‚»‚̉ž—p(2):CüŠîŽ¿‚ð—p‚¢‚½ƒvƒ‰ƒCƒ}[L’·”½‰ž‚Ì‘¬“x˜_“I‰ðÍ
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•\‘èCüDNA‚̍y‘f“I‡¬–@‚Æ‚»‚̉ž—p(1):CüƒvƒŠƒ“ƒkƒNƒŒƒIƒVƒhŽOƒŠƒ“Ž_‚̍‡¬‚ÆPCR‚É‚¨‚¯‚éŠîŽ¿“Á«
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