Enabling Technology Project

E12 Technology Development of High Efficient Bio Production by Innovative Cell Control Method and Breeding Method

Outline of the area

Akihiko Kondo Professor, Kobe University
Akihiko Kondo
Professor, Kobe University

Biomass such as plants and algae directly contribute to the reduction of greenhouse gases by fixing carbon dioxide. Its decomposition products and metabolites become raw materials for bioethanol, bioplastics, basic compounds, etc. and it can also substitute for petroleum. Furthermore, biotechnology can promote energy saving by deriving compounds difficult to prepare by conventional chemical methods and introducing bioprocesses. However, there are various problems in increased bio-production, such as derivation of excellent varieties, high-speed screening, maintaining traits in the real environment, difficulty in designing metabolism, differences between in lab scale and actual production scale culture, costs of cultivation and product purification.
On the other hand, by combining cutting-edge technologies such as synthetic biology, genome editing and synthesis technology, high-speed screening technology and AI / IT technology, innovative cell control and breeding become possible, shortening the period for social implementation.
In this project, we aim to achieve higher efficiency and lower cost of material production through developing innovative cell control and breeding method by collaboration among technology areas and by constructing cells that can increase the biomass production amount and enable high-efficiency production of bio-derived chemical products.


Metabolic Engineering of Cyanobacteria for Fermentative Production of Succinate and Lactate

Takashi Osanai
Associate professor, Meiji University

Bio-based succinate is a promising feedstock for the substitution of fossil fuels. Cyanobacteria are a group of bacteria performing oxygenic photosynthesis. In this study, we perform genetic engineering of cyanobacterial transcriptional regulators and circadian clocks for succinate production. Metabolomic approaches are important for clarifying the metabolic status of cyanobacteria to improve the productivity of succinate.

Genetic Engineering of Cyanobacterial Transcriptional Regulators and Circadian Clocks for Succinate Production


Development of a Robust and Biologically Contained Culturing Method of Microalgae Using Phosphite

Ryuichi Hirota
Associate Professor, Hiroshima University

Most organisms can not assimilate phosphite with a phosphorus oxidation state of +3. In this project, we will develop a robust and selective cultivation method of microalgae using phosphite dehydrogenase. Furthermore, we will apply this method to biocontainment that makes algae growth and survival dependent on phosphite.

Development of a robust cultivation method of microalgae using phosphite