MEMBERS

  • Dr.Masakazu Agetsuma

    Masakazu Agetsuma

    Associate Professor
    National Institute for Physiological Sciences

    Title

    Reading and manipulating fear memory - towards reconstruction of information processing mechanism for higher brain function

    Standard type Period：3years

    Summary

    The cerebral cortex plays important roles in cognition and behavior, while how its complex neural networks process the information for those functions is still unclear. Towards the deeper understanding of the mechanisms, we focus on the "fear memory" stored in the cerebral cortex, and investigate it via “observation” and “manipulation” of the neural activities with using cutting edge optical tools. We aim to unravel the "minimal building blocks" for fear memory, with suggesting the further medical applications to fear-related mental disorders.

    Research Results

    7/Apr/2016
    Oral Presentation: "Spatial and temporal network dynamics are co-regulated by parvalbumin-positive interneurons in mouse primary visual cortex" at the Cold Spring Harbor Laboratory meeting “Neural Circuit 2016”（Cold Spring Harbor）USA.
    May/2014
    Oral Presentation: “Functional contribution of different interneuron subtypes to cortical network synchrony” at the 79th Cold Spring Harbor Symposium on Quantitative Biology –Cognition　 (Cold Spring Harbor, USA)
    14/Jan/2014
    Karnani M., Agetsuma M., Yuste R.“A Blanket of Inhibition: Functional Inferences from Dense Inhibitory Connectivity”Current Opinion in Neurobiology 26, 96-102 (2014)
    13/Nov/2013
    Oral Presentation: "Functional contribution of different interneuron subtypes to cortical network synchrony" at the　43rd Annual Meeting of the Society for Neuroscience,San Diego,USA
  • Dr.Ryosuke Enoki

    Ryosuke Enoki

    Web site

    Assistant Professor
    Graduate School of Medicine, Photonic Bioimaging Section
    Hokkaido University

    Title

    Imaging, Control, and Reconstruction of Cellular Network in Master Circadian Clock

    Standard type Period：3years

    Summary

    Master circadian clock in mammals is located in the suprachiasmatic nucleus (SCN) of the brain. The SCN is the multi-oscillator system in which cellular network plays critical roles in expressing robust and coherent circadian rhythms. The present study will carry out to understand the fundamental mechanisms of cellular network in the SCN by utilizing various approaches, such as long-term optical imaging, cell/region-specific optogenetic control, and mathematical modeling. I will establish in-vivo optical imaging from deep region of the brain in behaving animals and analyze causal relationship between the cellular network activities and animal behavior (the final output).

    Research Results

    7/Mar/2017
    Enoki R, Oda Y, Mieda M, Ono D, Honma S, Honma KI."Synchronous circadian voltage rhythms with asynchronous calcium rhythms in the suprachiasmatic nucleus"Proc Natl Acad Sci U S A. 2017 Mar 7. pii: 201616815.
    5/Oct/2015
    Yoshikawa T, Nakajima Y, Yamada Y, Enoki R, Watanabe K, Yamazaki M, Sakimura K, Honma S, Honma KI. "Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus." European Journal of Neuroscience. 2015;42(9):2678-89.
    7-9/Sep/2015
    Oral Presentation: "Large-scale time lapse voltage imaging in neuronal networks of the suprachiasmatic nucleus." and "Multifunctional imaging of circadian rhythms in the suprachiasmatic nucleus" at the Asian Forum on Chronobiology in 2015. Sapporo, Japan
    5/Sep/2015
    Yoshikawa T, Nakajima Y, Yamada Y, Enoki R, Watanabe K, Yamazaki M, Sakimura K, Honma S, Honma KI."Spatiotemporal profiles of arginine vasopressin transcription in cultured suprachiasmatic nucleus" European Journal of Neuroscience, 2015 Nov;42(9):2678-89.
    2-6/Aug/2015
    Oral Presentation: "Multicolor imaging of circadian rhythms in the suprachiasmatic nucleus." at the European Biological Rhythms Society (EBRS)/World Congress of Chronobiology (WCC) meeting, (Manchester United Kingdom)
    4/Aug/2015
    Invited Lecture: "Multicolor imaging of circadian rhythms in the suprachiasmatic nucleus" at European Biological Rhythms Society (EBRS)/World Congress of Chronobiology (WCC), Manchester, UK.
    15/Nov/2014
    Presentation: "Large-scale imaging of circadian calcium rhythm in the suprachiasmatic nucleus" at the symposium"Network-mediated encoding of circadian time: the suprachiasmatic nucleus (SCN) from genes to neurons to circuits and back" in Society for Neuroscience（Washington DC, USA)
    15/Nov/2014
    Presentation: "Single-cell resolution fluorescence imaging of functional neuronal networks in the circadian master clock" at the Society for Neuroscience (Washington DC)
    12/Nov/2014
    Marco Brancaccio, Ryosuke Enoki, Cristina Mazuski,Jeff Jones, Jennifer Evans,Abdelhalim Azzi. "Network-mediated encoding of circadian time: the suprachiasmatic nucleus (SCN) from genes to neurons to circuits and back (review article)" Journal of Neuroscience, 2014 Nov 12;34(46):15192-9. doi: 10.1523/JNEUROSCI.3233-14.2014.
    27/Jul/2014
    Invited Lecture: “Large-scale, high-resolution fluorescence imaging of circadian rhythm in the master circadian clock” at the Joint Annual Meeting of the Japanese Society for Mathematical Biology (JSMB) and the Society for Mathematical Biology (SMB) (Osaka, Japan)
    May/2014
    Ryosuke Enoki, Sato Honma, Ken-ichi Honma“Imaging Circadian Calcium Rhythm in the Suprachiasmatic Nucleus” Dynamics of Circadian Oscillation in the SCN (edited by Honma KI), 2014; 37-49.
    15-19/Feb/2014
    Invitation Lecture : "Role of Rhythmic Calcium Signaling in SCN Circadian Pacemaker Function" at the 58th Annual Meeting, Plat Form Session, Biophysical Society,San Francisco, California,USA
    9－14/Nov/2013
    Oral Presentation: "Large-scale imaging of calcium rhythm in the suprachiasmatic nucleus" at the　43rd Annual Meeting of the Society for Neuroscience,San Diego,USA
  • Dr.Masato T. Kanemaki

    Masato T. Kanemaki

    Web site

    Professor
    Div. of Molecular Cell Engineering
    National Institute of Genetics

    Title

    Basic technology development for generation of degron mutants

    Standard type Period：3years

    Summary

    I have developed a new degron technology by applying an auxin-dependent degradation pathway of plants to none-plant cells. By using the auxin-inducible degron (AID) technology, it is now possible to deplete a protein of interest in less than one hour after addition of auxin to culture medium. In this project, I am going to develop methods to generate mammalian degron mutants, in which the expression level of an endogenous protein can be controlled. I am also going to try to improve the AID technology to make it better.

    Research Results

    26/Mar/2016
    Toyoaki Natsume, Tomomi Kiyomitsu, Yumiko Saga, and Masato T. Kanemaki "Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors" Cell Reports 2016 Apr 5;15(1):210-218. doi: 10.1016/j.celrep.2016.03.001.
    2016
    Devrekanli A, Kanemaki MT. "Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes." Methods in Molecular Biology, 1369, 257-278 (2016)
    19/Nov/2015
    Oral Presentation: "A short-homology-mediated tagging method for generation of human conditional mutants by use of the auxin-inducible degron (AID) technology" at the Conference on Transposition and Genome Engineering 2015, Nara, Japan
    2/Nov/2015
    Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H "Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines" Genes to Cells, 20, 1059-1076, 2015
    10/Oct/2015
    Oral Presentation: "Artificial destruction of active DNA replication forks reveals the HR-dependent fork recovery involving MCM8-9" at the 74th Japanese Cancer Association Annual Meeting, Nagoya, Japan.
    4/Sep/2015
    Invited Lecture: "Artificial destruction of active DNA replication forks reveals the HR-dependent fork recovery involving MCM8-9" at the Cold Spring Harbor Meeting on DNA replication and Genome Maintenance. Clod Spring Harbor Laboratories, NY, USA.
    1/Apr/2015
    Sanuki Y, Kubota Y, Kanemaki MT, Takahashi TS, Mimura S, Takisawa H. "RecQ4 promotes the conversion of the pre-initiation complex at a site-specific origin for DNA unwinding in Xenopus egg extracts." Cell Cycle, 14, 1010-1023, 2015
    20/Jan/2015
    Sanuki Y, Kubota Y, Kanemaki MT, Takahashi TS, Mimura S, Takisawa H. "RecQ4 promotes the conversion of the pre-initiation complex at a site-specific origin for DNA unwinding in Xenopus egg extracts" Cell Cycle 2015 Apr 3;14(7):1010-23. doi: 10.1080/15384101.2015.1007003.
    7/Nov/2014
    Samejima K, Ogawa H, Ageichik AV, Peterson KL, Kaufmann SH, Kanemaki MT, Earnshaw WC. "Auxin-induced rapid degradation of Inhibitor of Caspase Activated DNase (ICAD) induces apoptotic DNA fragmentation, caspase activation and cell death: A cell suicide module" Journal of Biological Chemistry 2014 Nov 7;289(45):31617-23. doi: 10.1074/jbc.M114.583542.
    29/Oct/2014
    Presentation: "A hidden DNA synthesis that contributes integrity of genomic DNA" at the US-Japan DNA repair meeting in Tokushima, Japan
    2/Sep/2014
    Nishimura K, Kanemaki MT.“IRapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID)” Current Protocols in Cell Biology, 64, 20.9.1-20.9.16, 2014
    19-23/May/2014
    Oral Presentation: “FThe Mcm8-9 complex functions in homologous recombination repair by promoting DNA strand synthesis after strand invasion” at the Abcam meeting on Mechanisms of Recombination (Alicante, Spain)
    8/Apr/2014
    Invited Lecture: “A hidden DNA synthesis in vertebrate cells” at the Seminar, MRC Genome Damage and Stability Centre, University of Sussex, UK
    7/Apr/2014
    Invited Lecture: “AWavelength sensitivity and depth perception mechanism in a jumping spider” at the Seminar in Clare Hall Institute, Cancer Research UK (London, UK)
    28/Feb/2014
    Rodriguez-Bravo V, Maciejowski J, Corona J, Buch HK, Collin P, Kanemaki MT, Shah JV,Jallepalli PV."Nuclear Pores Protect Genome Integrity by Assembling a Premitotic and Mad1-Dependent Anaphase Inhibitor" Cell, 156, 1017-1031, 2014
    5/Feb/2014
    Oral Presentation: "A crossover between DNA replication and recombination in vertebrate cells" at the International Conference, Kyoto, 2014: DNA replication, repair and transcription; coupling mechanisms and chromatin dynamics for genome integrity
    28/Dec/2012
    Kanemaki MT. "Frontiers of Protein Expression Control with Conditional Degrons." Pflugers Archive - European Journal of Physiology, 465, 419-425 (2013)
  • Dr.Koki Kamiya

    Koki Kamiya

    Researcher
    Artificial cell membrane system group
    Kanagawa Academy of Science and Technology

    Title

    Construction of a self-reproducible artificial cell model by using asymmetric lipid liposomes

    Standard type Period：3years

    Summary

    The asymmetric distribution of phospholipids in the extracellular and intracellular leaflets of natural cell membranes facilitates fundamental cellular functions, including signal transduction and cell division. In this study, I focus on the construction of a self-reproducible artificial cell model, which exhibits the cellular functions of cell fission and intracellular vesicular traffic. The artificial cells will be produced by reconstituting appropriate concentrations of biomolecules into asymmetric lipid liposomes by using various methods. The development of such an artificial cell model will provide new opportunities to identify the elementary reactions involved in various cellular functions and to create minimal cells.

    Research Results

    2016
    Kiyomitsu T. "Analyzing Spindle Positioning Dynamics in Cultured Cells." Methods in Molecular Biology. 2016
    13/Jul/2016
    Koki Kamiya,Ryuji Kawano,Toshihisa Osaki,Kazunari Akiyoshi,Shoji Takeuchi "Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes." Nature Chemistry 2016 Sep;8(9):881-9.
    24/Mar/2016
    Natsume T, Kiyomitsu T, Saga Y, and Kanemaki MT. "Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors." Cell Reports.15(1):210-8.
    27/Jan/2016
    Oral Presentation: " Construction of a self-reproducible artificial cell model by using asymmetric lipid liposomes"at the 29th International Conference on MEMS 2016, Shanghai China
    15/Sep/2015
    Oral Presentation: "Preparation of artificial cell models by bottom-up approach" at the 53rd Annual Meeting of the Biophysical Society of Japan. Kanazawa, Japan.
    26-30/Oct/2014
    Presentation: "SINGLE-CHANNEL CURRENT MEASUREMENT OF A CONNEXIN HEMICHANNEL EXPRESSED USING AN IN VITRO PROTEIN SYNTHESIS SYSTEM"at the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences in San Antonio, USA
    29/Jan/2014
    Oral Presentation: "RECONSTITUTION AND FUNCTION OF MEMBRANE PROTEINS INTO ASYMMETRIC GIANT LIPOSOMES BY USING A PULSED JET FLOW" and "MECHANICAL CELL PAIRING SYSTEM BY SLIDING PARYLENE RAILS" at the 27th IEEE International Conference on　Micro Electro Mechanical Systems(MEMS 2014) in San Francisco, California,USA
  • Dr.Tomomi Kiyomatsu

    Tomomi Kiyomitsu

    Assistant Professor
    Graduate School of Science
    Nagoya University

    Title

    Develop a method to control cell division types for the understanding of cell fate regulation and multi-cellular organization

    Standard type Period：3years

    Summary

    During the development in multi-cellular organisms, cells coordinate symmetric and asymmetric cell division to increase cell number and cell types, respectively. The type of cell division is determined in part by the positioning of the mitotic spindle within the cell. In this study, I will develop a method to control spindle position using optogenetic systems. With this technique, I will manipulate the localization of cortical force generating molecules in a single cell and multi-cellular context, and reveal the principles by which cells control cell division types and daughter cell fate.

    Research Results

    　
    22/May/2016
    Tomomi Kiyomitsu　"Analyzing Spindle Positioning Dynamics in Cultured Cells."　Methods Mol Biol. 2016;1413:239-52.
    20/Apr/2016
    Tomomi Kiyomitsu was awarded the Young Scientists' Prize on the Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology.
    17/Mar/2016
    Toyoaki Natsume, Tomomi Kiyomitsu, Yumiko Saga, and Masato T. Kanemaki "Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors" Cell Report 2016 Apr 5;15(1):210-218. doi: 10.1016/j.celrep.2016.03.001.
    26/Dec/2014
    Tomomi Kiyomitsu "Mechanisms of daughter cell-size control during cell division" Trends in Cell Biology (DOI: 10.1016/j.tcb.2014.12.003)
    31/Oct/2014
    Oral Presentation: "Cortical dynein and asymmetric membrane elongation coordinately position the spindle in anaphase" at the Dynein 2013 International Workshop (Kobe)
  • Dr.Nobuyasu Koga

    Nobuyasu Koga

    Associate Professor
    Institute for Molecular Science

    Title

    De novo design of allosteric proteins: Toward design and control of cellular functions

    Standard type Period：3years

    Summary

    Allosteric proteins perform switch-like regulation of their functions by changing their conformations upon ligand binding or light irradiation. For understanding the allosteric mechanisms, I will design allosteric proteins from scratch using computational methods and biochemical experiments. Furthermore, I aim to establish the rational methods for designing allosteric proteins and create novel functional proteins that contribute to designing and controlling cellular functions.

    Research Results

    5/Nov/2015
    Oral Presentation: "A synthetic approach to understanding protein molecules." at the JST CREST-PRESTO joint International Symposium, Tokyo Univ.
    Oct/2015
    Yu-Ru Lin, Nobuyasu Koga, Rie Koga, Gaohua Liu, Amanda F. Clouser, Gaetano T. Montelione, David Baker "Control over overall shape and size in de novo designed proteins" Proceedings of the National Academy of Sciences,2015 Oct 6;112(40):E5478-85.
    22/Sep/2015
    Yu-Ru Lin, #*Nobuyasu Koga, Rie Tatsumi-Koga, Gaohua Liu, Amanda F. Clouser, Gaetano T. Montelione, *David Baker ( # Yu-Ru Lin and Nobuyasu Koga contributed equally. ) "Control over overall shape and size in de novo designed proteins," Proc. Natl. Acad. Sci. USA, 112(40), E5478-5485, 2015
    17/Sep/2015
    Invited Lecture: "Design of ideal protein structures" at the 15th KIAS Conference on Protein Structure and Function, Korea Institute for Advanced Study, Seoul, Korea.
    12/Jun/2015
    Invited Lecture: "Protein design from scratch for understanding principles for folding" at the Supramolecular Dynamics at the interface of Chemistry and Biology, IMS Asian International Symposium, Institute for Molecular Science.
  • Dr.Mitsumasa Koyanagi

    Mitsumasa Koyanagi

    Associate Professor
    Graduate School of Science
    Osaka City University

    Title

    Spatio-temporally precise control of intracellular signaling molecules in a color-dependent manner

    Standard type Period：3years

    Summary

    Many cellular functions are controlled by the concentration changes of intracelluar signaling molecules. In this study, we try to develop useful optogenetic tools that can control intracellular signaling molecules in a color-dependent manner, which allow us to spatio-temporally control cellular and physiological functions more precisely than ever before. With the tools, we can reproduce and also disturb cellular functions, which may provide a clue to how cellular functions construct living systems.

    Research Results

    17/Aug/2016
    Tomohiro Sugihara, Takashi Nagata, Benjamin Mason, Mitsumasa Koyanagi, Akihisa Terakita "Absorption Characteristics of Vertebrate Non-Visual Opsin, Opn3." PLoS ONE 11(8):e0161215
    6/Nov/2015
    Hisao Tsukamoto, Yoshihiro Kubo, David L. Farrens, Mitsumasa Koyanagi, Akihisa Terakita, Yuji Furutani. "Retinal Attachment Instability Is Diversified among Mammalian Melanopsins." Journal of Biological Chemistry 290(45):27176-87
    22/Oct/2015
    Emi Kawano-Yamashita1, Mitsumasa Koyanagi, Seiji Wada, Hisao Tsukamoto, Takashi Nagata, Akihisa Terakita. "Activation of Transducin by Bistable Pigment Parapinopsin in the Pineal Organ of Lower Vertebrates." PLOS ONE 10(10): e0141280
    15/Sep/2015
    Koyanagi M, Wada S, Kawano-Yamashita E, Hara Y, Kuraku S, Kosaka S, Kawakami K, Tamotsu S, Tsukamoto H, Shichida Y, Terakita A. "Diversification of non-visual photopigment parapinopsin in spectral sensitivity for diverse pineal functions." BMC Biology (2015), 13:73.
    2015
    Akihisa Terakita1, Takashi Nagata1, Tomohiro Sugihara1, Mitsumasa Koyanagi "Optogenetic Potentials of Diverse Animal Opsins" Optogenetics pp 77-88 (Springer Japan)
    15/Sep/2015
    Mitsumasa Koyanagi, Seiji Wada, Emi Kawano-Yamashita, Yuichiro Hara, Shigehiro Kuraku, Shigeaki Kosaka, Koichi Kawakami, Satoshi Tamotsu, Hisao Tsukamoto, Yoshinori Shichida, Akihisa Terakita "Diversification of non-visual photopigment parapinopsin in spectral sensitivity for diverse pineal functions" BMC Biology 13:73 (2015)
    13/Sep/2015
    Oral Presentation: "Estimating spectral sensitivities of non-visual opsins based on biochemical responses of opsin-expressing cultured cells" at the 53rd Annual Meeting of the Biophysical Society of Japan. Kanazawa, Japan.
    9/Sep/2015
    Invited Lecture: "Introduction of animal rhodopsins and their optogenetic potentials" at the Annual Meeting on Photochemistry 2015.
    14/Jun/2015
    Oral Presentation:"Activation of Transducin by Bistable Pigment Parapinopsin in the Pineal Organ of Lower Vertebrates" at the FASEB Summer Research Conferences 2015 USA (Big Sky, Montana)
    8/Oct/2014
    Presentation: "Molecular properties of diverse and engineered bistable pigments and their optogenetic potential" at the 16th International Conference on Retinal Proteins in Shiga, Japan
    6/Oct/2014
    Presentation: "Identification of a visible light-sensitive pigment involved in the wavelength discrimination in the lamprey pineal organ","Molecular property and distribution of mammalian-like melanopsins in lamprey and hagfish" at the 16th International Conference on Retinal Proteins ・Shiga, Japan
    24/Sep/2014
    Sun L1, Kawano-Yamashita E1, Nagata T1, Tsukamoto H2, Furutani Y2, Koyanagi M3, Terakita A1. "Distribution of Mammalian-like Melanopsin in Cyclostome Retinas Exhibiting a Different Extent of Visual Functions" PLOS ONE 2014; 9(9):e108209. doi: 10.1371
    1/Aug/2014
    Invited Lecture:“A hidden DNA synthesis in vertebrate cells” at the Conference（New York, USA）
  • Dr.Yuki Sato

    Yuki Sato

    Lecturer
    Faculty of Medical Sciences
    Kyushu University

    Title

    Regulation and Reconstruction of Blood Vessel Networks by Blood Flow Manipulation

    Standard type Period：3years

    Summary

    Blood vessels display hierarchal network patterns corresponding to the blood volume in a central to peripheral manner. To understand the roles of blood flow in the vascular patterning process, we aim to experimentally manipulate the bloodstream by using an artificial heart pump. Through this manipulation, we aim to elucidate the correlation between blood flow and endothelial cell behavior, as well as simulate the process of vascular patterning. The ultimate goal of this study is to achieve complete control of blood vessel networks through manipulation of blood flow at a single point.

    Research Results

    15/Jan/2017
    Yuki Sato, Kei Nagatoshi, Ayumi Hamano, Yuko Imamura, David Huss, Seiichi Uchida, and Rusty Lansford.“Basal Filopodia and Vascular Mechanical Stress Organize Fibronectin into Pillars Bridging the Mesoderm-Endoderm Gap” Development,144(2):281-291.
    11/Dec/2015
    Invited Lecture: "Dynamic Imaging of Endothelial Cells and circulating blood in developing embryos."at the Cardiovascular and Metabolic Week 2015. (Kobe, Japan).
    13/Sep/2015
    Invited Lecture: "Basal Fillopodia and Vascular Pulsing Organize Fibronectin into Pillars That Bridge Somites and the Endoderm" at the Asia-Pacific Developmental Biology Conference 2015. China
    4/Jun/2015
    Oral Presentation: "Fibrillar Adhesion and Vascular Constriction Regulate Patterned Deposition of Fibronectin Pillars that Bridge Somites and the Endoderm" at the 48th Annual Meeting of the Japanese Society of Developmental Biologists. Japan
    17/Apr/2014
    Shoichiro Kanda, Shunsuke Tanigawa, Tomoko Ohmori, Atsuhiro Taguchi, Kuniko Kudo, Yutaka Suzuki, Yuki Sato, Shinjiro Hino, Maike Sander, Alan O. Perantoni, Sumio Sugano, Mitsuyoshi Nakao, and Ryuichi Nishinakamura “Sall1 Maintains Nephron Progenitors and Nascent Nephrons by Acting as Both an Activator and a Repressor.”Journal of the American Society of Nephrology 2014 Nov;25(11):2584-95.
  • Dr.Mito Shinohara

    Mito Shinohara

    Assistant professor
    Graduate School of Medicine
    Kyoto University

    Title

    Analysis of spermatogonial stem cell turnover and dynamics of spermatogenesis

    Standard type Period：3years

    Summary

    Spermatogonial stem cells are the source of sperm production throughout adult life, but their number is limited. Dynamics of spermatogonial stem cell turnover has remained unknown.This project is aimed to reveal the dynamics of spermatogonial stem cell turnover by in vivo clonal analysis. Dynamics of spermatogenesis will be reproduced by mathematical modeling. The dynamics of spermatogenesis will also be modulated by genetic manipulation.

    Research Results

    8/Aug/2016
    Kanatsu-Shinohara M, Naoki H, Shinohara T. "Nonrandom germline transmission of mouse spermatogonial stem cells." Dev Cell 2016, 38 (3): 248-61
    9/Aug/2016
    Takashi Tanaka, Mito Kanatsu-Shinohara, Zhenmin Lei, C.V. Rao, and Takashi Shinohara. "The luteinizing hormone-testosterone pathway regulates mouse spermatogonial stem cell self-renewal by suppressing WNT5A expression in Sertoli cells." Stem Cell Reports 2016 Aug 9;7(2):279-91.
    6/Apr/2016
    Mito Kanatsu-Shinohara, Hiroko Morimoto, and Takashi Shinohara "Fertility of male germline stem cells following spermatogonial transplantation in infertile mouse models." Biology of Reproduction 2016 May;94(5):112.
    17-19/Feb/2016
    Oral Presentation: "Enrichment of mouse spermatogonial stem cells by the stem cell dye CDy1"at International Symposium on Epigenome Dynamics and Regulation in Germ Cells. Kyoto, Japan
    1/Jan/2016
    Mito Kanatsu-Shinohara, Hiroko Morimoto, and Takashi Shinohara. "Enrichment of mouse spermatogonial stem cells by the stem cell dye CDy1." Biology of Reproduction (USA) 94(1):13. doi: 10.1095/biolreprod.115.135707.
    21/Oct/2015
    Tanaka, T., Kanatsu-Shinohara M, Hirose, M, Ogura, A, Shinohara, T. "Pluripotent cell derivation from male germline cells by suppression of Dmrt1 and Trp53." J. Reprod. Dev. USA.2015 Oct 21;61(5):473-84.
    25/Aug/2015
    Tanaka, T, Kanatsu-Shinohara M, Shinohara T "The CDKN1B-RB1-E2F1 pathway protects mouse spermatogonial stem cells from genomic damage." J. Reprod. Dev.USA. 2015;61(4):305-16.
    6/May/2015
    Morimoto H, Kanatsu-Shinohara M, Shinohara T."ROS-generating oxidase Nox3 regulates the self-renewal of mouse spermatogonial stem cells."Biol. Reprod.USA.2015 Jun;92(6):147
    10/Mar/2015
    Takashima S, Kanatsu-Shinohara, Tanaka T, Morimoto H, Inoue K, Ogonuki N, Jijiwa M, Takahashi M, Ogura A, ○Shinohara T. "Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal" Stem Cell Reports（USA）2015 Mar 10;4(3):489-502. doi: 10.1016/j.stemcr.2015.01.010.
    10/Sep/2014
    Kanatsu-Shinohara M, Ogonuki N, Matoba S, Morimoto H, Ogura A, Shinohara T. "Improved serum- and feeder-free culture of mouse spermatogonial stem cells." Biology of Reproduction USA 2014. pii: biolreprod.114.122317.
    17/Jul/2014
    Kanatsu-Shinohara M, Onoyama I, Nakayama KI, Shinohara T. "Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal." Proc Natl Acad Sci U S A. ;111(24):8826-31. 2014
  • Dr.Kaoru Sugimura

    Kaoru Sugimura

    Web site

    Assistant professor
    Institute for Integrated Cell-Material Sciences (WPI-iCeMS)
    Kyoto University

    Title

    Design and control of tissue morphogenesis

    Standard type Period：3years

    Summary

    How do cells push and pull each other to trigger precise deformations of a tissue when shaping the body? The answer to this central question is essential for understanding the development of animal forms. By combining physical modeling, mechanical and genetic perturbations along with live imaging and Bayesian force-inference, I will identify the underlying physical principles required to regulate morphogenesis. Furthermore, I will develop a method to manipulate deformation and force of a cell group aiming at a constitutive understanding of tissue morphogenesis.

    Research Results

    16/Aug/2016
    Oral Presentation: "Actin interacting protein 1 senses the extrinsic stretching force and orients cell rearrangement in Drosophila wing." at the 2016 Santa Cruz Developmental Biology Meeting.USA
    15/Jan/2016
    Kaoru Sugimura, Pierre-Francois Lenne, Francois Graner. "Measuring forces and stresses in situ in living tissues." Development 143(2):186-96. doi: 10.1242/dev.119776.
    9/Jan/2016
    Oral Presentation: "Dissecting the mechanical control of Drosophila wing shape determination." at the JAPAN Q-bio week NIG International Symposium - Tokyo Symposium: Force, Information and Dynamics: X factors shaping living systems.Tokyo Japan.
    12/Dec/2015
    Boris Guirao, Stephane U. Rigaud, Floris Bosveld, Anais Bailles, Jesus Lopez-Gay, Shuji Ishihara, Kaoru Sugimura, Francois Graner and Yohanns Bellaiche. "Unified quantitative characterization of epithelial tissue development." eLife 2015 Dec 12;4. pii: e08519. doi: 10.7554/eLife.08519.
    Apr/2015
    Guirao B, Rigaud SU, Bosveld F, Bailles A, Lopez-Gay J, Ishihara S, Sugimura K, Graner F, Bellaiche Y. "Unified quantitative characterization of epithelial tissue development." eLife 2015, 4, e08519.
    31/Jul/2014
    Mihoko Kajita, Kaoru Sugimura, Atsuko Ohoka, Jemima Burden, Hitomi Suganuma, Masaya Ikegawa, Takashi Shimada, Tetsuya Kitamura, Masanobu Shindoh, Susumu Ishikawa, Sayaka Yamamoto, Sayaka Saitoh, Yuta Yako, Ryosuke Takahashi, Takaharu Okajima, Junichi Kikuta, Yumiko Maijima, Masaru Ishii, Masazumi Tada,Yasuyuki Fujita "Filamin acts as a key regulator in epithelial defence against transformed cells" Nature Communications, 5: 4428 (2014)
    31/Jul/2014
    Oral Presentation: “Bayesian force inference and its application to tissue morphogenesis” at the 37th Naito Conference "Bioimaging-a paradigm shift for the life sciences" (Hokkaido, Japan)
    22/May/2014
    Oral Presentation: “Bayesian force inference from cell shape and connectivity II” at the "Forces in tissues" workshop (Paris, France)
    26/Apr/2014
    Oral Presentation: “Bayesian Force Inference and Its Application to Tissue Morphogenesis” at the BIT's 4th Annual World Congress of Molecular & Cell Biology 2014 (Dalian, China)
  • Dr.Haruki Takeuchi

    Haruki Takeuchi

    PRESTO Researcher
    Graduate School of Pharmaceutical Sciences
    University of Tokyo

    Title

    Unraveling Mechanisms of Activity-Dependent Neural Circuit Formation by Manipulating spike trains

    Standard type Period：3years

    Summary

    In mammals, neural circuits are assembled by genetically-determined coarse targeting process and subsequent activity-dependent refinement process. However, it remains unclear how neural activity represented as electrical spike trains contributes to the assembly of neural circuits. In this study, I seek to understand the instructive role for neural activity in neural circuit formation by applying optogenetics.

    Research Results

    5-8/Mar/2017
    Oral Presentation:“Patterned spontaneous activity regulates OR-specific axon sorting”at Keystone Symposia "Synapses and Circuits" (New Mexico, USA)
    12-16/Nov/2016
    Oral Presentation:“Differential expression of axon-sorting molecules in mouse olfactory sensory neurons”at Society for Neuroscience 2016 (San Diego, USA)
    28-29/Oct/2016
    Oral Presentation: “Patterned spontaneous activity regulates OR-specific axon sorting”at KoSCL symposium (Seoul, Korea).
    20-24/Sep/2016
    Oral Presentation: “An instructive role for spontaneous neural acitivity in glomerular map formation”at CSHL meeting "Axon Guidance, Synapse Formation & Regeneration" (New York, USA).
    20/Jul/2016
    Dr.Haruki Takeuchi was awarded the Japan Neuroscience Society Young Investigator Award in 2016.
    10/Jun/2016
    Ihara N, Nakashima A, Hoshina N, Ikegaya Y and Takeuchi H “Differential expression of axon-sorting molecules in mouse olfactory sensory neurons”　 Eur. J. Neurosci 　2016, Vol.44(3), 1998-2003　Featured in World biomedical Frontiers[ISSN:2328-0166]
    8/Jun/2016
    Oral Presentation: "Differential expression of axon-sorting molecules in mouse olfactory sensory neurons." at the 17th international symposium on olfaction and taste(Yokohama, Japan)
    13/Feb/2017
    Eerdunfu, Ihara N, Bao Ligao, Ikegaya Y, and Takeuchi H. “Differential timing of neurogenesis underlies the dorsal-ventral topographic projection of olfactory sensory neurons” Neural.Dev.
    18/Mar/2014
    Takeuchi H,Sakano H "Neural map formation in the mouse olfactory system" Cellular and Molecular Life Sciences Mar 18 doi: 10.1007/s00018-014-1597-0
    12/Sep/2013
    Nakashima A, ※Takeuchi H, Imai T, Saito H, Kiyonari H, Abe T, Chen M, Weinstein LS, Yu CR, Storm DR, Nishizumi H, and Sakano H “Agonist-Independent Receptor Activity Regulates Axon Targeting of Olfactory Neurons.”Cell,　 2013, Vol.154 1314-1325 ※ equally contributed　
  • Dr.Takanori Takebe

    Takanori Takebe

    Web site

    Associate Professor
    Dept.of Pediatrics, Cincinnati Children's Hospital Medical Center
    University of Cincinnat

    Title

    In vitro human organ generation by reconstituting spatiotemporal control of multilple cellular/tissue interactions

    Standard type Period：3years

    Summary

    We recently showed that the recapitulation of early organogenetic cellular interactions can initiate three-dimensional organ bud morphogenesis in vitro from human pluripotent stem cells. In this project, we will optimize the multiple parameters including biophysical stimuli to precisely reconstitute the complex and fluidic developmental regulation of spatial and temporal changes in inter-cellular and tissue interactions. Finally, we will establish a novel method to grow functional human organ in vitro from stem cells for future industrial and clinical use.

    Research Results

    26/Oct/2016
    Dr.Takanori Takebe was awarded the 2016 NYSCF – Robertson Stem Cell Investigators.
    14/Oct/2016
    Invited Lecture: "De novo generation of diverse organ buds from stem cells." at the EMBO/ESBL Symposia 2016,Heidelberg,Germany
    20/Apr/2016
    Dr.Takanori Takebe was awarded the Young Scientists' Prize on the Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology.
    22-24/Mar/2016
    Invited Lecture: "Generating diverse organ buds towards therapy."at CiRA / ISSCR 2016 International Symposia | Kyoto, Japan.
    14/Mar/2016
    Invited Lecture: "Generation of Diverse Organ Buds from Human iPSCs."at French Society for Cell and Gene Therapy, Marseille, France.
    15/Feb/2016
    Kagimoto S, Takebe T*, Kobayashi S, Yabuki Y, Hori A, Hirotomi K, Mikami T, Uemura T, Maegawa J, Taniguchi H*: "Autotransplantation of monkey ear perichondrium-derived progenitor cells for cartilage reconstruction." Cell transplantation. (*Joint corresponding authors), 2016;25(5):951-62.
    6/Nov/2015
    Oral Presentation: "Generating diverse organ buds from stem cells."at the JST CREST-PRESTO joint international symposium, Tokyo, Japan
    22/Oct/2015
    Invited Lecture: "Human iPSC-derived organ bud based approaches." at the 112th ITC(INTERNATIONAL TITISEE CONFERENCE, Mainz Germany.
    8/Sep/2015
    Invited Lecture: "In vitro reconstructions and controls of multicellular structures" at the 26th CDB Meeting Mechanistic Perspectives of Multicellular Organization, Kobe, Japan.
    25/Jul/2015
    Invited Lecture: "De novo generation of liver buds from stem cells" at the International Conference of Future Hepatology at Kaohsiung, Kaohsiung Taiwan.
    15/Jul/2015
    Zhang R-R, Koike H, Takebe T."The visualization of human organogenesis from stem cells by recapitulating multicellular interactions." Hyper Bio Assembler for 3D Cellular Systems,Chapter 17.
    26/Jun/2015
    Invited Lecture:"De novo generation of diverse organ buds from iPSCs towards therapy" at the ISSCR 2015 ANNUAL MEETING, Stockholm Sweden.
    8/Jun/2015
    Invited Lecture: "Realization of human iPSC-derived liver bud transplantation therapy" at the Young Investigators’ Stem Cell Symposium 2015, Singapore.
    14-16/May/2015
    Invited Lecture: "Human iPSC-derived organ bud based approaches" at the 9th Aso International Meeting, Kumamoto.Japan.
    12/May/2015
    Invited Lecture: "De Novo Generation of Human Organ Bud from iPSC" at the Yale University, Digestive Diseases Research Seminar Series, Connecticut USA.
    22-26/Apr/2015
    Invited Lecture: "Regenerative approaches through developmental biology" at the EASL, 50th the International Liver Congress, Vienna Austria.
    20/Apr/2015
    Invited Lecture: "Realization of iPSC-organ bud transplantation therapy" at the Wellcome Trust-Medical Research Council, Cambridge Stem Cell Institute, Cambridge UK.
    16/Apr/2015
    Takebe T1, Enomura M, Yoshizawa E, Kimura M2, Koike H, Ueno Y, Matsuzaki T, Yamazaki T, Toyohara T, Osafune K, Nakauchi H, Yoshikawa H-Y, Taniguchi H "Vascularized And Complex Organ Buds From Diverse Tissues Via Mesenchymal Cell-Driven Condensation." Cell Stem Cell. 2015 May 7;16(5):556-65.
    6/Feb/2015
    Oral Presentation: "Liver organogenesis"at the ASTELLAS FRANCE Annual Meeting of Transplantation Club , France.
    11/Apr/2015
    Invited Lecture: "Human iPSC-derived organ bud based approaches towards clinical application." at the 8th Pan Pacific Symposium 2015 on Stem Cells and Cancer Research, Taiwan.
    12/Dec/2014
    Dr.Takanori Takebe was awarded the Promotion of Medical Science of the Kanae Foundation.
    26/Nov/2014
    Dr.Takanori Takebe was awarded the Baelz Prize of Boehringer Ingelheim GmbH.
    30/Sep/2014
    Invited Lecture: “De novo organ bud generation from stem cells. ”at the State-of-the-art lecture , Liver Center of the Digestive Health Center, Cincinnati Children’s Hospital Medical Center (CCHMC) (Cincinnati, Ohio, USA)
    14/Sep/2014
    Invited Lecture: “ 'Ad-Med' - Activating Healthcare Behaviour Through Communication.” at the Spikes Asia 2014 (Singapore)
    11/Sep/2014
    Invited Lecture: “ Vascularized and functional human liver from an iPSC-derived organ bud transplant. ”at the KEY Forum: From Stem Cells to Organs (Kumamoto, Japan)
    10/Sep/2014
    Takanori Takebe “Transient vascularization of transplanted human adult–derived progenitors promotes self-organizing cartilage” Jaurnal of Clinical Investigation, doi: 10.1172/JCI76443 2014
    14/Jun/2014
    Invited Lecture: “ 'Ad-Med' - Activating Healthcare Behaviour Through Communication.” at the Cannes Lions Health (Cannes, France)
    28/May/2014
    Invited Lecture: “Vascularized and functional human liver from an iPSC-derived organ bud transplant. ” at the 47th Annual Meeting for the Japanese Society of Developmental Biologists (Nagoya, Japan)
    29-30/Apr/2014
    Invited Lecture: “Realization of human iPSC-derived organ bud transplantation therapy. ”at the Whole Liver Replacement State-of-the-Science Summit (Cｈicago, USA)
    12-14/Apr/2014
    Invited Lecture: “Vascularized and functional human liver from an iPSC-derived organ bud transplant. ” at the 7th Pan Pacific Symposium on Stem Cells and Cancer Research (PPSSC) (Taiwan)
    7/Jan/2014
    Dr.Takanori Takebe was awarded the Sceince, 10 breakthrough of the year, 2013 Sceince, 10 breakthrough of the year, 2013
    20/Dec/2013
    Dr.Takanori Takebe was awarded the Discover Magazine, Top 5 science stories of 2013Discover Magazine, Top 5 science stories of 2013
    17/Dec/2013
    Oral Presentation: "Vascularized and functional human liver from an iPSC-derived organ bud transplant." at the 12TH US-JAPAN SYMPOSIUM ON DRUG DELIVERY SYSTEMS,Hawaii,USA
    25/Nov/2013
    Oral Presentation: "Vascularized and functional human liver from an iPSC-derived organ bud transplant." at the Small RNAs to Stem Cells & Epigenetic Reprogramming,Osaka
    12/Nov/2013
    Oral Presentation: "Vascularized and functional human liver from an iPSC-derived organ bud transplant. "at the 12th Congress International Xenotransplantation Association,Osaka
    23/Oct/2013
    Oral Presentation: "Vascularized and functional human liver from an iPSC-derived organ bud transplant "at the 5th Regenerative Medicine Seminar sponsored by JSPS and NRF（Konkuk University）Seoul
    23/Oct/2013
    Oral Presentation: "Vascularized and functional human liver from an iPSC-derived organ bud transplant "at the 7th International Cell Therapy Conference（Seoul）
    23/Oct/2013
    Oral Presentation: "Vascularized and functional human liver from an iPSC-derived organ bud transplant "at the 4th Regenerative Medicine Seminar sponsored by JSPS and NRF（Inha University） Korea
    4/Feb/2014
    Koike H, Ueno Y, Naito T, Shiina T, Nakata S, Ouchi R, Obana Y, Sekine K, Zheng Y.W.,Takebe T, Isono K, Koseki H, Taniguchi H "Ring1B Promotes Hepatic Stem/Progenitor Cell Expansion via Simultaneous Suppression of Cdkn1a and Cdkn2a"Hepatology 2014 Jul;60(1):323-33. doi: 10.1002/hep.27046.
    23/Jan/2014
    Takebe T, Zhang RR, Koike H, Kimura M, Yoshizawa E, Enomura M, Koike N, Sekine K, Taniguchi H."Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant."Nature Protocols 9(2):396-409, 2014
    20/Mar/2014
    Mizuno M, Kobayashi S, Takebe T, Kan H, Yabuki Y, Matsuzaki T, Yoshikawa HY, Nakabayashi S, JeongIk L, Maegawa J, Taniguchi H "Reconstruction of joint hyaline cartilage by autologous progenitor cells derived from ear elastic cartilage."Stem cells 32(3):816-821 2014
  • Dr.Yu Hayashi

    Yu Hayashi

    Associate Professor
    International Institute for Integrative Sleep Medicine
    University of Tsukuba

    Title

    Manipulation and analyses of REM sleep using transgenic mice

    Standard type Period：3years

    Summary

    REM sleep, which is the major source of dreams, is unique to mammals and birds and thus might regulate sophisticated brain functions. Its physiologic roles, however, remain a mystery. Our genetic identification of REM sleep-regulating cells has allowed artificial inhibition of REM sleep in mice. In this study, such mice will be utilized to reveal the functions of REM sleep and dreams. In the future, I aim to elucidate how the global brain activity during sleep or various other conditions affect individual cells or brain areas.

    Research Results

    30/May/2016
    Invited Lecture: "Genetic dissection of the neural circuitry controlling paradoxical (REM) sleep at the Lyon Neuroscience Research Center Seminar.Lyon, France.
    20/Nov/2015
    Yu Hayashi, Mitsuaki Kashiwagi, Kosuke Yasuda, Reiko Ando, Mika Kanuka, Kazuya Sakai, Shigeyoshi Itohara, "Cells of a common developmental origin regulate REM/non-REM sleep and wakefulness in mice." Science. 2015 Nov 20;350(6263):957-61. doi: 10.1126/science.aad1023. Epub 2015 Oct 22.　
    22/Oct/2015
    Yu Hayashi, Mitsuaki Kashiwagi, Kosuke Yasuda, Reiko Ando, Mika Kanuka, Kazuya Sakai, Shigeyoshi Itohara. "Cells of a common developmental origin regulate REM/non-REM sleep and wakefulness in mice." Science. 2015, 350, 957-961.
    11/Mar/2015
    Invited Lecture: "Genetic dissection of the REM (paradoxical) sleep circuit" at the Tokyo Metropolitan Institute of Medical Science. Tokyo, Japan.
    30/Jan/2015
    Suzuki A, Lee LJ, Hayashi Y, Muglia L, Itohara S, Erzurumlu RS, Iwasato T. "Thalamic adenylyl cyclase 1 is required for barrel formation in the somatosensory cortex" Neuroscience 2015 Apr 2;290:518-29. doi: 10.1016/j.neuroscience.2015.01.043.
    28/Sep/2014
    Oral Presentation: “How the brain generates REM sleep” at the Tsukuba Global Science Week 2014 (Tsukuba, Japan)
    26/Sep/2014
    Oral Presentation: “Genetic dissection of the REM-non-REM sleep switch” at the 68th Fujihara Seminar (Izu, Japan)
    3/Sep/2014
    Hiroyuki Arakawa, Ayumi Suzuki, Shuxin Zhao, Vassiliy Tsytsarev, Fu-Sun LO, Yu Hayashi, Shigeyoshi Itohara, Takuji Iwasato, and Reha Erzurumlu “Thalamic NMDA receptor function is necessary for patterning of the thalamocortical somatosensory map and for sensorimotor behaviors.” Journal of Neuroscience 2014 Sep 3;34(36):12001-14.
    9/Nov/2013
    Oral Presentation: "Genetic analysis of the roles of NMDA receptors in the parafascicular nucleus in sensory processing" at the　43rd Annual Meeting of the Society for Neuroscience,San Diego,USA
    22/Feb/2014
    Oral Presentation: "Genetic dissection of REM sleep circuitry" at the　1st National Taiwan University & University of Tsukuba Joint Conference, Taipei, Taiwan
  • Dr.Yosuke Morishima

    Yosuke Morishima

    Web site

    Group leader
    Division of Systems Neuroscience of Psychopathology Translational Research Center,University Hospital of Psychiatry
    University of Bern

    Title

    Development of methodological framework to regulate functional brain connectivity

    Standard type Period：3years

    Summary

    Brain processes information for behavioral guidance. It has been assumed that synchronizing neural activity in brain networks is associated with the efficacy of neural transmission. However, there is no causal evidence that proves the idea. To address this question, the current project aims to develop a methodological framework regulating brain networks and measuring their changes by using non-invasive brain stimulation. I further address the neural mechanisms of adaptive behavior by modulating the functional interactions of brain networks. I hope the method would be applied to the treatment of psychiatric disorders by improving impaired functional connectivity.

    Research Results

    20/Sep/2016
    Azade Dogan, Yosuke Morishima, Felix Heise, Carmen Tanner, Rajna Gibson, Alexander F. Wagner,Philippe N. Tobler "Prefrontal connections express individual differences in intrinsic resistance to trading off honesty values against economic benefits." Scientific Reports 2016 Sep 20;6:33263.
    9/Sep/2016
    Oral Presentation: "Synchronized tACS entrained oscillatory activity modulates long-range neuronal transmission efficacy" at the 6th International Conference on Transcranial Brain Stimulation 2016,Gottingen, Germany .
    29/Jul/2016
    Oral Presentation: "Entrained oscillatory activity modulates long-range neuronal transmission efficacy" at the Organization for Human Brain Mapping (OHBM) Annual Meeting 2016,Geneva,Switzerland.
    11/Mar/2016
    Oral Presentation: "Control of effective connectivity through transcranial alternating current stimulation" at the 4th BENESCO Winter Research Meeting, Wengen, Switzerland.
    4/Mar/2016
    Grit Hein, Yosuke Morishima, Susanne Leiberg, Sunhae Sul, and Ernst Fehr "The Brain’s Functional Network Architecture Reveals Human Motives." Science;351(6277):1074-8. doi: 10.1126/science.aac7992.　　
    23/Jan/2016
    Oral Presentation: "Entrained oscillatory activity modulates long-range neuronal transmission efficacy"at the 2016 Swiss Society for Neuroscience, Lausanne, Switzerland.
    22/Jan/2016
    Kristoffer D. Feher, Yosuke Morishima. "Concurrent Electroencephalography Recording During Transcranial Alternating Current Stimulation (tACS)" Journal of Visualized Experiments 2016 Jan 22;(107). doi: 10.3791/53527.
    12/Jan/2016
    Oral Presentation: "Entrained oscillatory activity modulates long-range neuronal transmission efficacy"at the 11th Apline Brain Imaging Meeting, Champery, Switzerland.
    4/Jun/2015
    Simon Schwab, Thomas Koenig, Yosuke Morishima, Thomas Dierks, Andrea Federspiel, Kay Jann "Discovering frequency sensitive thalamic nuclei from EEG microstate informed resting state fMRI" Neuroimage, Vol. 118, pp. 368-375, 2015
    17/Nov/2014
    Presentation: "A method for removing transcranial alternating current stimulation (tACS) artifacts from EEG data" at the Society for Neuroscience 2014 USA(Washington DC)
    26/Sep/2014
    Presentation: "Functional brain connectivity identifies human motives"at the Society for Neuroeconomics Meeting 2014 Miami, USA
    11/Jun/2014
    Presentation: "Individuals’ neural circuitry predicts their prosocial motives"at the Annual Meeting 2014 of Organization of Human Brain Mapping in Hamburg, Germany
    12/May/2014
    Presentation: "Reducing the error: Regional effects of HRF basis functions on EEG-microstate informed fMRI" at the Joint Annual Meeting ISMRM-ESMRMB 2014 in Milano, Italy
    9/Nov/2013
    Oral Presentation: "Neural mechanisms of context-dependent and independent altruism" at the　Invited seminar Center for Economic and Neuroscience, University of Bonn,Germany
  • Dr.Yoko Yamanishi

    Yoko Yamanishi

    Web site

    Professor
    Faculty of Engineering
    Kyushu University

    Title

    Manipulation and reconstruction of molecules with electrically-induced bubble injection knife

    Standard type Period：3years

    Summary

    In this research, bubbles whose interface attached high molecular materials such as gene can be injected to the aimed localized position with high efficiency. The phase state of the attached materials can be solid, liquid and gas phase. More importantly, the molecule can be concentrated with bubble shrinkage. By using the mechanism, the protein crystallization can be achieved. Our objectives are the establishments of a novel method of reconstruction of high molecule such as membrane protein.

    Research Results

    28/Nov/2016
    Oral Presentation: “Emerging Functions of Plasma-Induced Bubbles”at 2016 MRS Fall Meeting & Exhibit, Boston, U.S.A.
    18-20/Apr/2016
    Invited Lecture: “Emerging Functions of Electrically-induced Bubble Injector”at the 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)，Sendai, JAPAN.
    25/Jan/2016
    Oral Presentation:“High Dynamic Ablation and Injection by Electric Cavitation for Wide Range of Materials”at The 28th International Conference on Micro Electro Mechanical Systems (MEMS2016), Shanghai, CHINA.
    3/Dec/2015
    Oral Presentation: "Concentration and Extraction of Plasma-induced Radical Species for Protein Crystallization."at the MRS2015 Fall Meeting. Boston, USA.
    23/Nov/2015
    Oral Presentation: "Multiple-reagent Layer Laden High-speed Micro-bubbles in Extended Two-dimensional Microchip."at the 2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS2015), pp.73-76 . Nagoya, Japan.
    12/Nov/2015
    Oral Presentation: "Fabrication and Development of Micro-magnetic tags to Transport Biological Information." at the 28th International Microprocesses and Nanotechnology Conference, Toyama, Japan.
    28/Oct2015
    Oral Presentation: "Extract of Radical Species by Criculating Reactive Interface of Microfludic Chip for Protein Crystallization."at Proc. of the 19 th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (μ-TAS 2015), pp.251-253, Korea.
    22/Sep/2015
    Oral Presentation:“Processing Biological Material by Combination of Cavitation and Plasma Irradiation."at AEPSE2015, 22p-A(150)-7, (2015.9.22) Jeju Island , Korea.
    23,24/Jun/2015
    Oral Presentation: "Investigation of Molecular Condensation on Air-liquid Interface for Protein Crystallization", "Plasma-cavitation Pencil Cutter for Powerful Micro-Processing”at the Transducers 2015, Anchorage, Alaska.
    8/Jun/2015
    Oral Presentation: "Evaluation of Attractive Force on Air-Liquid Interface for Protein Crystallization","Rounding of Protein Crystal by High-Speed Bubble Jet in Microfluidic Chip" and "Transportation of Multiple-reagent Using Adsorption and Contraction Force of Micro-bubble"at the ISMM 2015, Kyoto, Japan.
    22/Jan/2015
    Presentation:"Carving of Protein Crystal by High-speed Micro-bubble Jet Using Micro-fluidic Platform"at the 28th International Conference on Micro Electro Mechanical Systems (MEMS2015) in Estoril, Pertugal
    21/Jan/2015
    Presentation: "Minimally Invasive Needle-free Bubble Injector for Gene Therapy"at the 28th International Conference on Micro Electro Mechanical Systems (MEMS2015)in Estoril, Pertugal
    10/Nov/2014
    Presentation: "Minimally-invasive Local Injection by Electrically-driven Narrow Orifice Channel" at the International Symposium on Micro-Nano Mechatronics and Human Science 2014 (MHS2014) in Nagoya University
    29/Oct/2014
    S. Takasawa, Y. Fujiwara, T. Kobayashi, , M. Oomura, H. Kamegawa and Yoko Yamanishi. "Micro-bubble Ring Generation by Electrically-driven High-speed Bubble Strike under Micro-fluidic Environment" Proc. of the 18th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (micro-TAS 2014) , pp.1548-1550, 2014.
    27/Oct/2014
    K. Takahashi, W. Kawaguchi, Y. Hamano, S. Hosoda, Y. Arakawa, and Yoko Yamanishi. "Electrically Induced Bubble Capillary-poration" Proc. of the 18th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (micro-TAS 2014) , pp.1392-1394, 2014.
    6/Nov/2014
    Presentation: "Contraction and Condensation of Protein Molecule by Electrically-Induced Bubbles for Crystallization"at the 27th International Microprocesses and Nanotechnology Conference (MNC2014) in Fukuoka
    20/May/2014
    Oral Presentation: “Local injection using regent-laden micro-bubbles”“Electrically-driven micro-bubbles assisted protein crystallization”at the 5th International conference on Plasma Medicine (ICPM5), Nara, Japan.
    2013
    Hiroki Kuriki, Yoko Yamanishi, Shinya Sakuma, Satoshi Akagi, Fumihito Arai “Local Ablation of a Single Cell Using Micro/Nano Bubbles” ournal of Robotics and Mechatronics, 25-3, (2013), pp.476-483.
    12/Nov/2013
    Oral Presentation: “Disintegration and Conveyance of Dielectric Barrier Discharge-Generated Micro-Plasma Ball under Water”at the 2013 International Symposium on Micro-NanoMechatronics and Human Science.
    29/Oct/2013
    Oral Presentation: "Multiphase-laden Gas-liquid Interface Injection for the Versatile Gene Transfer" at the 17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (micro-TAS)in Freiburg, Germany
    30/Oct/2013
    Oral Presentation: "Protein Crystallization Induced by Electrically Driven Bubble Knife" at the 17th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences (micro-TAS)in Freiburg,Germany
    11/Nov/2013
    Oral Presentation: "Electrically-induced bubble knife for protein crystallization and processing" at the 2013 International Symposium on Micro-Nano　Mechatronics and Human Science (MHS2013), pp.154-157 (2013)in Nagoya

Researchers

  • 1st Phase
  • 2nd Phase
  • 3rd Phase