Takahide Kon

Degree

Ph.D.

Position, Research site

Professor, Department of Biological Sciences, Graduate School of Science, and Faculty of Science, Osaka University

URL

http://www.bio.sci.osaka-u.ac.jp/en/

Research Field

Biophysics, Structural biology, Cell biology

• Education, Research career
• Publication
• Books,Patent,Award

Key Publication

1. Imai, H., Shima, T., Sutoh, K., Walker, ML., Knight, PJ., Kon, T., Burgess, SA.:Direct observation shows superposition and large scale flexibility within cytoplasmic dynein motors moving along microtubules. Nat Commun. 2015 Sep 14;6:8179. doi: 10.1038/ncomms9179
2. Uchimura S, Fujii T, Takazaki H, Ayukawa R, Nishikawa Y, Minoura I, Hachikubo Y, Kurisu G, Sutoh K, Kon T, Namba K, Muto E. A flipped ion pair at the dynein-microtubule interface is critical for dynein motility and ATPase activation. J Cell Biol. 2015 Jan 19;208(2):211-22. doi: 10.1083/jcb.201407039. Epub 2015 Jan 12.
3. Ikuta J, Kamisetty NK, Shintaku H, Kotera H, Kon T, Yokokawa R. Tug-of-war of microtubule filaments at the boundary of a kinesin- and dynein-patterned surface. Sci Rep. 2014 Jun 13;4:5281. doi: 10.1038/srep05281.
4. Anthony Roberts, Takahide Kon, Peter Knight, Kazuo Sutoh, Stan Burgess.:Functions and mechanics of dynein motor proteins. Nature Rev. Mol. Cell. Biol. 2013, 14:713-726. doi: 10.1038/nrm3667.
5. Roberts, A.J., Malkova, B., Walker, M.L., Sakakibara, H., Numata, N., Kon, T., Ohkura, R., Edwards, T.A., Knight, P.J., Sutoh, K., Oiwa, K., Burgess, S.A.:ATP-Driven Remodeling of the Linker Domain in the Dynein Motor. Structure 2012, 20:1670-1680.
6. Kon, T., Oyama, T., Shimo-Kon, R., Imamula, K., Shima, T., Sutoh, K., Kurisu, G. The 2.8 A crystal structure of the dynein motor domain. Nature. 2012, 484: 345-350.
7. Kon, T., Sutoh, K., Kurisu, G. X-ray structure of a functional full-length dynein motor domain. Nature StructMolBiol. 2011 18: 638-642.
8. Kon, T., Shima, T., Sutoh, K. Protein engineering approaches to study the dynein mechanism using a Dictyostelium expression system. MethodsCellBiol. 2009, 92: 65-82.
9. Kon, T., Imamula, K., Roberts, A.J., Ohkura, R., Knight, P.J., Gibbons, I.R., Burgess, S.A., Sutoh, K. Helix sliding in the stalk coiled coil of dynein couples ATPase and microtubule binding. Nature Struct.Mol.Biol. 2009, 16: 325-333.
10. Roberts, A.J., Numata, N., Walker, M.L., Malkova, B., Kon, T., Ohkura, R., Arisaka, F., Knight, P.J., Sutoh, K., Burgess, S.A. AAA+ ring and linker swing mechanism in the dynein motor. Cell. 2009, 136: 485-495.
11. Mizuno, N., Narita, A., Kon, T., Sutoh, K., Kikkawa M. Three-dimensional structure of cytoplasmic dynein bound to microtubules. Proc.Natl.Acad. Sci.USA. 2007, 104: 20832-20837.
12. Imamula, K., Kon, T., Ohkura, R., Sutoh, K. The coordination of cyclic microtubule association/dissociation and tail swing of cytoplasmic dynein. Proc.Natl.Acad. Sci.USA. 2007, 104: 16134-16139.
13. Shima, T., Kon, T., Imamula, K., Ohkura, R., Sutoh, K. Two modes of microtubule sliding driven by cytoplasmic dynein. Proc.Natl.Acad. Sci.USA. 2006, 103: 17736-17740.
14. Kon, T., Mogami, T., Ohkura, R., Nishiura, M., Sutoh, K. ATP hydrolysis cycle-dependent tail motions in cytoplasmic dynein. Nature Struct.Mol.Biol. 2005, 12: 513-519.
15. Kon, T., Nishiura, M., Ohkura, R., Toyoshima, Y.Y., Sutoh, K. Distinct Functions of Nucleotide-Binding/Hydrolysis Sites in the Four AAA Modules of Cytoplasmic Dynein. Distinct Functions of Nucleotide-Binding/Hydrolysis Sites in the Four AAA Modules of Cytoplasmic Dynein. Biochemistry. 2004, 43: 11266-11274.

Publication

1. 「細胞中心方向への輸送エンジン「ダイニン」の構造と運動機構」昆隆英、実験医学増刊. 2014, 32(10): 221-224.
2. 昆隆英，栗栖源嗣.:細胞中心方向輸送エンジン：細胞質ダイニンの構造と運動メカニズム. 生化学. 2013, 85(4): 272-275.
3. Roberts, A.J., Malkova, B., Walker, M.L., Sakakibara, H., Numata, N., Kon, T., Ohkura, R., Edwards, T.A., Knight, P.J., Sutoh, K., Oiwa, K., Burgess, S.A., ATP-Driven Remodeling of the Linker Domain in the Dynein Motor. Structure 2012, 20:1670-1680.
4. Kon, T., Shima, T., Sutoh, K., Dynein | Cytoplasmic Dynein: its ATPase Cycle and ATPase-dependent Structural Changes. Comprehensive Biophysics, 1st Edition (Editor in Chief: Edward Egelman), 2012, Vol4：4.19, Elsevier
5. Shima, T., Sutoh, K., Kon, T., Functional analysis of the dynein motor domain. In Handbook of Dynein (Amos, L and Hirose, K., Eds), 2012. pp43-62. Pan Stanford Publishing Pte Ltd.
6. 島 知弘, 須藤和夫, Takahide Kon、細胞質ダイニンの構造と機能のモジュール性、生物物理. 2011, 51: 118-123.
7. Numata, N., Shima, T., Ohkura, R., Kon, T., Sutoh, K., C-sequence of the Dictyostelium cytoplasmic dynein participates in processivity modulation, FEBSLett. 2011, 585:1185-1190.
8. Inoue, T., Kon, T., Ohkura, R., Yamakawa, H., Ohara, O., Yokota, J., Sutoh, K., BREK/LMTK2 is a myosin VI-binding protein involved in endosomal membrane trafficking. GenesCells. 2008, 13: 483-495
9. Numata, N., Kon, T., Shima, T., Imamula, K., Mogami, T., Ohkura, R., Sutoh, K., Sutoh, K., Molecular mechanism of force generation by dynein, a molecular motor belonging to the AAA+ family., Biochem. Soc.Trans. 2008, 36: 131-135.
10. Yokokawa, R., Tarhan, M.C., Kon, T., Fujita, H., Simultaneous and Bidrectional Transoprt of Kinesin-Coateed Microspheres and Dynein-Coated Microspheres on Polarity-Oriented Microtubules., Biotechnol.Bioeng. 2008, 101: 1-8.
11. Yokokawa, R., Murakami, T., Sugie, T., and Kon, T., Polarity Orientation of Microtubules Utilyzing Dynein-Based Gliding Assay., Nanotechnology 2008, 19: 125505.
12. Takahide Kon、ダイニンの構造とモーター活性の解析、生物物理. 2007, 47: 384-389.
13. Mogami, T., Kon, T., Ito, K., Sutoh, K., Kinetic characterization of tail swing steps in the ATPase cycle of Dictyostelium cytoplasmic dynein. J.Biol.Chem. 2007, 282: 21639-21644.
14. Ito, K., Ikebe, M., Kashiyama, T., Mogami, T., Kon, T., Yamamoto, K., Kinetic mechanism of the fastest motor protein, Chara myosin., J.Biol.Chem. 2007, 282: 19534-19545.
15. Shima, T., Imamula, K., Kon, T., Ohkura, R., Sutoh, K., Head-head coordination is required for the processive motion of cytoplasmic dynein, an AAA+ molecular motor. J. Struct.Biol. 2006, 156: 182-189.
16. Inoue, T., Kon, T., Ajima, R., Ohkura, R., Tani, M., Yokota, J., Sutoh, K., MYO18B interacts with the proteasomal subunit Sug1 and is degraded by the ubiquitin-proteasome pathway. Biochem.Biophys.Res.Comm. 2006, 342: 829-834.
17. Yokokawa, R., Yoshida, Y., Takeuchi, S., Kon, T., and Fujita, H., Unidirectional transport of a bead on a single microtubule immobilized in a submicrometre channel. Nanotechnology2006, 17: 289-294.
18. Isogawa, Y., Kon, T., Inoue, T., Ohkura, R., Yamakawa, H., Ohara, O., Sutoh, K., The N-terminal domain of MYO18A has an ATP-insensitive actin-binding site. Biochemistry. 2005, 44: 6190-6196.
19. Yokokawa, R., Yoshida, Y., Takeuchi, S., Kon, T., Sutoh, K., and Fujita, H., Evaluation of Cryopreserved Microtubules Immobilized in Microfluidic Channels for Bead-Assay-Based Transportation System. IEEETrans.Adv. Packag. 2005, 28: 577-583.
20. Yokokawa, R., Takeuchi, S., Kon, T., Nishiura, M., Sutoh, K., Fujita, H., Unidirectional transport of kinesin-coated beads on microtubules oriented in a microfluidic device. Nano letters. 2004, 4: 2265-2270.
21. Asano, Y., Mizuno, T., Kon, T., Nagasaki, A., Sutoh, K., Uyeda, T.Q., Keratocyte-like locomotion in amiB-null Dictyostelium cells. CellMotil.Cytoskeleton. 2004, 59: 17-27.
22. Nishiura, M., Kon, T., Shiroguchi, K., Ohkura, R., Shima, T., Toyoshima, Y.Y., Sutoh, K., A single-headed recombinant fragment of Dictyostelium cytoplasmic dynein can drive the robust sliding of microtubules. J.Biol.Chem. 2004, 279: 22799-22802.
23. Yokokawa, R., Takeuchi, S., Kon, T., Nishiura, M., Ohkura, R., Edamatsu, M., Sutoh, K., Fujita, H., Hybrid nanotransport system by biomolecular linear motors, IEEE/ASME J.Microelectromech. Syst. 2004, 13: 612-619
24. Suzuki, T., Iwasaki, T., Uzawa, T., Hara, K., Nemoto, N., Kon, T., Ueki, T., Yamagishi, A., Oshima, T., Sulfolobus tokodaii sp. nov. (f. Sulfolobus sp. strain 7), a new member of the genus Sulfolobus isolated from Beppu hot springs, Japan, Extremophiles. 2002, 6: 39-44.
25. Kon, T., Nemoto, N., Oshima, T., Yamagishi, A., Effects of a squalene epoxidase inhibitor, Terbinafine, on ether lipid biosynthesis in a thermoacidophilic archaeon, Thermoplasma acidophilum. J.Bacteriol. 2002, 184: 1395-1401.
26. Kon, T., Adachi, H., Sutoh, K., amiB, a novel gene required for the growth/differentiation transition in Dictyostelium. GenesCells. 2000, 5: 43-55.
27. 25 Saito, J., Kon, T., Nagasaki, A., Adachi, H., Sutoh, K., Dictyostelium TRFA homologous to yeast Ssn6 is required for normal growth and early development. J.Biol.Chem. 1998, 273: 24654-24659.
28. Iwasaki, T., Suzuki, T., Kon, T., Imai, T., Urushiyama, A., Ohmori, D., Oshima, T., Novel zinc-containing ferredoxin family in thermoacidophilic archaea. J.Biol.Chem. 1997, 272: 3453-3458.