To better study influenza virus infection in vivo, we generated influenza A viruses (Color-flu viruses), which highly and stably produce multi-spectral fluorescent proteins in infected mice while maintaining their original pathogenicity. We demonstrated that Color-flu viruses are useful for flow cytometric analyses and for different types of microscopy.
JST Strategic Basic Research Programs
Discovery and development of next-generation vaccines and antivirals against influenza
ERATO Kawaoka Infection-induced Host Responses Project
Satoshi Fukuyama, Hiroaki Katsura, Dongming Zhao, Makoto Ozawa, Tomomi Ando, Jason E. Shoemaker, Izumi Ishikawa, Shinya Yamada, Gabriele Neumann, Shinji Watanabe, Hiroaki Kitano, and Yoshihiro Kawaoka. “Multi-spectral fluorescent reporter influenza viruses (Color-flu) as powerful tools for in vivo studies”. Nature Communications 6, Article number: 6600, Published online 25 March 2015, doi: 10.1038/ncomms7600..
Yoshihiro Kawaoka, D.V.M., Ph.D.
Division of Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo
4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639
Department of Research Project, JST
K’s Goban-cho 7, Goban-cho, Chiyoda-ku, Tokyo102-0076