Analyzing protein-protein interaction is essential to elucidate finely tuned life phenomena. Prof. Ueda and his co-workers have developed a novel technology, named “Firefly luminescent intermediate based Protein Interaction Assay (FlimPIA)”, to detect these interactions by combining two complementary luciferase mutants. Highly sensitive and specific, this technology demonstrated under-1-second detection of several known protein-protein interactions in a concentration-dependent manner.
FlimPIA may be applied to drug discovery and clinical diagnostics as a convenient, high speed and specific assay.
JST-SENTAN (Development of Advanced Measurement and Analysis Systems), Technology Development Type, “Development of novel protein-protein interaction detecting technology with high speed and sensitivity”
Yuki Ohmuro-Matsuyama, Kota Nakano, Aoi Kimura, Keiichi Ayabe, Masaki Ihara, Takeshi Wada, and Hiroshi Ueda.
“A Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases”
Published online on August 1st 2013, in “Analytical Chemistry”.
Professor Hiroshi UEDA, Ph.D.
Chemical Resources Laboratory, Tokyo Institute of Technology
Masae SUGAWARA, Ph.D.
Department of Industry-Academic Alliance, Office of Advanced Measuring Technology,
Japan Science and Technology Agency