Hanaoka and his colleagues have successfully developed new red fluorescent reagent "CaTM-2" to visualize cytoplasmic calcium ion concentration inside living cells.
Calcium ions are involved in many biological phenomena such as muscle contraction and neural activity. However, appropriate red fluorescent reagent with uniform cytoplasmic distribution has not been available.
Hanaoka’s "CaTM-2" reagent demonstrated red visualization of neural firing in rat brain slice, together with cell body staining by Acridine orange in green color.
"CaTM-2" allows researchers to access close elucidation of calcium involving cell reactions by multi-color imaging.
JST-SENTAN (Development of Advanced Measurement and Analysis Systems), Technology Development Type, “Development of novel fluorophores and their derivatization to innovative fluorescent probes for the next generation”
Published online on February 25th 2013, in“Angewandte Chemie International Edition”, a German scientific journal.
Kenjiro HANAOKA, Ph.D.
Graduate School of Pharmaceutical Sciences, the University of Tokyo
Masae SUGAWARA, Ph.D.
Department of Industry-Academic Alliance, in charge of Advanced Measuring Technology,
Japan Science and Technology Agency