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JST Press Release

19th February 2013
Japan Science and Technology Agency (JST)
5-3, Yonbancho, Chiyoda-ku, Tokyo 102-8666
URL http://www.jst.go.jp/EN/index.html

Strong red fluorescent reagent for cytoplasmic calcium ion concentration analysis
- A bright step to multi-color imaging of biological phenomena -

Hanaoka and his colleagues have successfully developed new red fluorescent reagent "CaTM-2" to visualize cytoplasmic calcium ion concentration inside living cells.

Calcium ions are involved in many biological phenomena such as muscle contraction and neural activity. However, appropriate red fluorescent reagent with uniform cytoplasmic distribution has not been available.

Hanaoka’s "CaTM-2" reagent demonstrated red visualization of neural firing in rat brain slice, together with cell body staining by Acridine orange in green color.

"CaTM-2" allows researchers to access close elucidation of calcium involving cell reactions by multi-color imaging.

Researcher Information

JST-SENTAN (Development of Advanced Measurement and Analysis Systems), Technology Development Type, “Development of novel fluorophores and their derivatization to innovative fluorescent probes for the next generation”

Journal Information

Published online on February 25th 2013, in“Angewandte Chemie International Edition”, a German scientific journal.
doi: 10.1002/anie.201210279


[About Research]
Kenjiro HANAOKA, Ph.D.
Associate Professor,
Graduate School of Pharmaceutical Sciences, the University of Tokyo

[About Program]
Akira KUBO
Department of Industry-Academic Alliance, in charge of Advanced Measuring Technology,
Japan Science and Technology Agency


JST, an integrated organization of science and technology in Japan, establishes an infrastructure for the entire process from the creation of knowledge to the return to the society. For more information, visit http://www.jst.go.jp/EN/