[Miki Bundo] Analysis of somatic mutations in brain cells using a cell nuclei separation technique

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Miki Bundo

Miki Bundo

Faculty of Life Sciences, Kumamoto University
Associate Professor
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Outline

Recent studies have revealed that the genome of brain cells contains several kinds of somatic mutations, such as chromosomal aneuploidy and insertion of retrotransposons. These brain cell-specific somatic mutations may play an important role in the development of psychiatric diseases. My colleagues and I recently reported that the copy number of the retrotransposon LINE-1 is higher in the neurons of patients with schizophrenia than in the neurons of healthy controls. In this proposed project, I plan to identify the brain cell types showing accumulation of somatic mutations in patients with schizophrenia. For this, I will establish techniques for isolation of single-cell nucleus from different types of human brain cells and for sequencing the genomic DNA from the isolated single nucleus. I will then conduct a case-control study using postmortem brain samples from patients with schizophrenia and controls without schizophrenia to clarify the pathogenesis of schizophrenia.

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