Research Project Outline
The combination of rapid freezing technique and phase contrast transmission electron microscopy (TEM) has enabled us to observe biological samples without heavy metal staining, making the traditional lengthy specimen preparation obsolete. We will go further by developing a method to observe live samples at normal temperature and normal atmosphere. A combination of three elements, the pulsed photo-electron gun, the environmental cell and phase contrast TEM, supported by a novel design for the electron lens is the major focus in the development.