Accomplishments

Original Papers

  1. Mitsuhiro Kimura, Takeshi Yoshizumi, Keiji Numata, A centrifugation-assisted peptide-mediated gene transfer method for various high-throughput analyses. Plant Biotechnology, 36, 1, pp49-52, 2019.
  2. Mio Hikawa, Kazuyo Nishizawa, Yutaka Kodama, Prediction of prospective leaf morphology in lettuce based on intracellular chloroplast position. Scientia Horticulturae, 251, pp20-24, 2019.
  3. Keiko Midorikawa, Yutaka Kodama, Keiji Numata, Vacuum/Compression Infiltration-mediated Permeation Pathway of a Peptide-pDNA Complex as a Non-Viral Carrier for Gene Delivery in Planta. Scientific Reports, 9, 1, pp271, 2019.
  4. Laddawan Saelim, Nobuhiro Akiyoshi, Tian Tian Tan, Ayumi Ihara, Masatoshi Yamaguchi, Ko Hirano, Makoto Matsuoka, Taku Demura, Misato Ohtani, Arabidopsis Group IIId ERF proteins positively regulate primary cell wall-type CESA genes. Journal of Plant Research, 132, 1, pp117-129, 2019.
  5. Kazusato Oikawa, Md Monirul Islam, Yoko Horii, Takeshi Yoshizumi, Keiji Numata, Screening of a cell-penetrating peptide library in Escherichia coli: relationship between cell penetration efficiency and cytotoxicity. ACS Omega, 3, 12, pp16489-16499, 2018.
  6. Yuto Takenaka, Yoichiro Watanabe, Mathias Schuetz, Faride Unda, Joseph Lee Hill, Pawittra Phookaew, Arata Yoneda, Shawn D. Mansfield, A. Lacey Samuels, Misato Ohtani, Taku Demura, Patterned Deposition of Xylan and Lignin is Independent from that of the Secondary Wall Cellulose of Arabidopsis Xylem Vessels. Plant Cell, 30, 11, pp2663-2676, 2018.
  7. Kosuke Tsuchiya, Keiji Numata, Protease-Catalyzed Polymerization of Tripeptide Esters Containing Unnatural Amino Acids: α,α-Disubstituted and N-Alkylated Amino Acids. Green Polymer Chemistry: New Products, Processes, and Applications. Edited by H. N. Cheng, Richard A. Gross, and Patrick B. Smith,, Chapter 7, pp95–105, ACS Symposium Series, Vol.1310, 2018.
  8. Takaaki Miyamoto, Kousuke Tsuchiya, Keiji Numata, Block copolymer/plasmid DNA micelles postmodified with functional peptides via thiol/maleimide conjugation for efficient gene delivery into plants. Biomacromolecules, 20, 2, pp653-661, 2018.
  9. Keiji Numata, Yoko Horii, Kazusato Oikawa, Yu Miyagi, Taku Demura, Misato Ohtani, Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus. Scientific Reports, 8, 10966, 2018.
  10. Ryota Konno, Hiroyuki Tanaka, Yutaka Kodama, SKLPT imaging: efficient in vivo pre-evaluation of genome-editing modules using fluorescent protein with peroxisome targeting signal. Biochemical and Biophysical Research Communications, 503, 1, pp235-241, 2018.
  11. Yuta Fujii, Ayaka Yoshimura, Yutaka Kodama, A novel orange-colored bimolecular fluorescence complementation (BiFC) assay using monomeric Kusabira-Orange protein. BioTechniques, 64, 4, pp153-161, 2018.
  12. Misato Ohtani, Harunori Kawabe, Taku Demura, Evidence that thiol-based r edox state is critical for xylem vessel cell differentiation. Plant Signaling & Behavior, 13, e1428512, 2018.
  13. Takeshi Yoshizumi, Kazusato Oikawa, Jo-Ann Chuah, Yutaka Kodama, Keiji Numata, Selective gene delivery for integrating exogenous DNA into plastid and mitochondrial genomes using peptide-DNA complexes. Biomacromolecules, 19, 5, pp1582–1591, 2018.
  14. Jo-Ann Chuah, Keiji Numata, Stimulus-Responsive Peptide for Effective Delivery and Release of DNA in Plants. Biomacromolecules, 19, 4, pp1154-1163, 2018.
  15. Masahiro Noguchi, Masayuki Fujiwara, Ryosuke Sano, Yoshimi Nakano, Yoichiro Fukao, Misato Ohtani, Taku Demura, Proteomic analysis of xylem vessel cell differentiation in VND7-inducible tobacco BY-2 cells by two-dimensional gel electrophoresis. Plant Biotechnol, 35, 1, pp31-37, 2018.
  16. Tian Tian Tan, Hitoshi Endo, Ryosuke Sano, Kurata Tetsuya, Masatoshi Yamaguchi, Ohtani Misato, Demura Taku, Transcription factors VND1-VND3 contribute to cotyledon xylem vessel formation, Plant Physiol, 176,1, pp773-789, 2018.
  17. Harunori Kawabe, Misato Ohtani, Tetsuya Kurata, Tomoaki Sakamoto, Taku Demura, Protein S-nitrosylation regulates xylem vessel cell differentiation in Arabidopsis. Plant Cell Physiol, 59, 1, pp17-29, 2018.
  18. Hiroyuki Tanaka, Mayuko Sato, Yuka Ogasawara, Noriko Hamashima, Othmar Buchner, Andreas Holzinger, Kiminori Toyooka, Yutaka Kodama. Chloroplast aggregation during the cold-positioning response in the liverwort Marchantia polymorpha L. Journal of Plant Research, 130, 6, pp1061-1070, Nov 2017.
  19. Yasuhide Osaki, Yutaka Kodama, Particle bombardment and subcellular protein localization analysis in the aquatic plant Egeria densa. PeerJ, 5, e3779, 2017.
  20. Kousuke Tsuchiya, Keiji Numata, Chemoenzymatic synthesis of polypeptides containing the unnatural amino acid 2-aminoisobutyric acid. Chemical Communications, 53, pp7318-7321, 2017.
  21. Yuta Fujii,Hiroyuki Tanaka,Naotake Konno,Yuka Ogasawara,Noriko Hamashima,Saori Tamura,Satoshi Hasegawa,Yoshio Hayasaki,Koji Okajima,Yutaka Kodama, Phototropin perceives temperature based on the lifetime of its photoactivated state, Proc Natl Acad Sci U S A, 114, 34 :9206–9211, 2017.
  22. Takeshi Yoshizumi, Miwa Yamada, Mieko Higuchi-Takeuchi, Ken’ichiro Matsumoto, Seiichi Taguchi, Minami Matsui, Keiji Numata, Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants. Plant Biotechnology, 34, 1, pp39-43, 2017.
  23. Jo-Ann Chuah, Yoko Horii, Keiji Numata, Peptide-Derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants. Journal of Visualized Experiments (JoVE), 118, e54972, 2016.

Review(peer-reviewed)

  1. Tian Tian Tan TT, Taku Demura, Misato Ohtani. Creating vessel elements in vitro: towards a comprehensive understanding of the molecular basis of xylem vessel element differentiation, Plant Biotechnol, Accepted.
  2. Takuya Katashima, Ali D. Malay, Keiji Numata. Chemical modification and biosynthesis of silk-like polymers (Invited mini-review). Current Opinion in Chemical Engineering, 24, pp61-68, 2019.
  3. Kousuke Tsuchiya, Yu Miyagi, Takaaki Miyamoto, Prashant G. Gudeangadi, Keiji Numata. Chapter 8 Synthesis of Polypeptides. "Enzymatic Polymerization towards Green Polymer Chemistry", Springer, 2019.
  4. Kosuke Tsuchiya, Keiji Numata. Protease-Catalyzed Polymerization of Tripeptide Esters Containing Unnatural Amino Acids: α,α-Disubstituted and N-Alkylated Amino Acids. Green Polymer Chemistry: New Products, Processes, and Applications. Edited by H. N. Cheng, Richard A. Gross, and Patrick B. Smith. Chapter 7 pp95–105, ACS Symposium Series, Vol. 1310, 2018.
  5. Chris Holland, Keiji Numata, Jelena Rnjak-Kovacina, Philipp Seib. The biomedical use of silk: past, present, future (Invited review). Advanced Healthcare Materials, 8(1), 1800465, 2018.
  6. Misato Ohtani, Taku Demura. The quest for transcriptional hubs of lignin biosynthesis: beyond the NAC-MYB- gene regulatory network model, Curr Opin Biotech, 56, pp82-87, 2018.
  7. Yuta Fujii, Yutaka Kodama. Refinements to light sources used to analyze the chloroplast cold-avoidance response over the past century (Invited article addendum). Plant Signaling & Behavior, 13(3), 2018.
  8. Misato Ohtani. Plant snRNP biogenesis: a perspective from the nucleolus and Cajal bodies, Front Plant Sci,8,2184, 2018.
  9. Kosuke Tsuchiya, Keiji Numata. Chemoenzymatic synthesis of polypeptides as functional and structural materials (Invited review). Macromolecular Bioscience, 1700177, 2017.

Review & notes(domestic)

  1. 樋口(竹内)美栄子.「海洋性紅色細菌によるバイオプラスチック生産」、工業材料、2019年3月号 p46-50.
  2. 沼田圭司.「バイオイメージングによるオルガネラ改変機構の解明とアグリバイオへの展望」、アグリバイオ、2019年2月号.
  3. 土屋康佑、沼田圭司.「化学酵素重合を利用した機能性ポリペプチドの合成」、化學工業、2018年12月号, pp929-934.
  4. 沼田圭司.「沼田圭司博士に聞く 「生物由来の素材から革新的な材料をつくる」、現代化学、2018年12月号, pp40-44.
  5. 児玉豊、「植物が温度を感じる仕組み〜ようやく発見!植物の温度センサー分子〜」、化学と生物、57巻1号 pp21-28.
  6. 沼田圭司.「アミノ酸から構成される高分子材料の創成」、アグリバイオ、2018年8月号.
  7. 沼田圭司、土屋康佑、児玉豊.「オルガネラ反応クラスターが切り拓くバイオマス社会」、植物の生長調節、53巻1号 pp80-83.
  8. 秋吉信宏, 出村拓, 大谷美沙都. 「植物の通道細胞進化を転写因子から読み解く」、化学と生物, 56巻5号 pp353-363.
  9. 大谷美沙都. 「pre-mRNA スプライシングが制御する植物の発生・環境応答・器官再生」、BSJ-Review、2017年9月、8B.
  10. 出村拓, 大谷美沙都. 「構造材料としての木質高分子の生合成メカニズムの理解」、高分子、2017年6月、Vol66.
  11. 大谷美沙都. 「植物の器官再生におけるRNA代謝制御の役割」、生物科学、2017年4月、Vol68.
  12. 沼田圭司. 「ペプチド法:人工制限酵素をはじめとしたタンパク質の導入技術として」、植物の生長調節、2017年5月、Vol52、No1.

Invited lecture & seminar

  1. Yutaka Kodama, Cold stress sensing by phototropin to control chloroplast positioning. 35th IPSR International Symposium, Kurashiki Geibunkan/ Ai-Theater, Japan, March 5, 2019.
  2. Yutaka Kodama, Phototropin is thermosensory protein for chloroplast movement. Japan-Taiwan Plant Biology 2019, Nagoya University, Japan, March 15, 2019.
  3. 児玉豊、植物における光受容体による温度感知.日本生態学会 第66回大会、神戸国際会議場・神戸国際展示場、2019年3月17日.
  4. 児玉豊、葉緑体運動に関わる温度感知機構の解明.第60回日本植物生理学会年会、名古屋大学 東山キャンパス、2019年3月14日.
  5. 児玉豊、低温誘導性の葉緑体配置を制御する温度センサーの発見.日本作物学会 第247回講演会、筑波大学中地区第二エリア、2019年3月29日.
  6. Keiji Numata, Genetic modification of mitochondria. Hubrecht Seminar, Hubrecht Institute, Germany, February 14, 2019.
  7. Keiji Numata, Polypeptides as functional nanomaterials toward achieving organellar engineering and artificial spider silk. International Conference on Functional Nanomaterials (ICFNM)-2019, India, February 22-25, 2018.
  8. Keiji Numata, Peptide-mediated gene/protein delivery system homing to target organelles of intact plants. Seminar, Pennsilvania State University, United States, January 17, 2018.
  9. Keiji Numata, Polypeptides as biological structural materials. Special MEC Seminar, California Institute of Technology, United States, January 15, 2018.
  10. 沼田圭司、ペプチドを利用した植物バイオ技術.植物科学シンポジウム、東京大学弥生講堂 一条ホール、2018年12月12日.
  11. 児玉豊、植物の温度感知:光受容体フォトトロピンは温度センサーである.第3回 Biothermology Workshop、岡崎コンファレンスセンター、2018年12月25日.
  12. Keiji Numata, Polypeptide materials for agricultural and engineering applications. BASF NAO Familyday, Innovation Campus Shanghai, China, November 1, 2018.
  13. Misato Ohtani, Possible “RNA processing checkpoint” for cell potency regulation in plants. Japan-Australia RNA meeting, Hokkaido University, Japan, November 5-7, 2018.
  14. 児玉豊、低温誘導性の葉緑体運動:青色光受容体フォトトロピンは温度受容体である.東京農工大学セミナー、東京農工大学、2018年11月22日.
  15. Kousuke Tsuchiya, Chemoenzymatic synthesis of functional polypeptides. The 6th International Conference on Smart Systems Engineering 2018, MIRAI Hall, Yamagata, 2018年10月11日.
  16. 大谷美沙都、植物細胞の増殖・分化能を制御するRNA代謝制御系.  第六回富山RNA倶楽部、富山大学五福キャンパス、2018年9月18日.
  17. 樋口美栄子、沼田圭司、海洋性紅色光合成細菌の窒素固定及び二酸化炭素固定反応を利用したポリヒドロキシアルカン酸の生産. 第67回高分子討論会、北海道大学、2018年9月12日.
  18. Keiji Numata, Enzymatic synthesis of structural protein-like polypeptides. 25th Anniversary meeting of the BioEnvironmental Polymer Society 2018, Rensselaer Polytechnic Institute, United States, August 15-17, 2018.
  19. Keiji Numata, Chemo-enzymatic synthesis of silk and elastin-like polypeptides as renewable materials. Symposium on The Rational Design of Multifunctional Renewable-Resourced Materials, ACS National Meeting, United States, August19-23, 2018.
  20. Misato Ohtani, From Arabidopsis to poplar; our challenge of woody biomass engineering with transgenic poplars. International Symposium on Forest Tree Molecular Biology and Biotechnology (FTMB2018), Harbin Kempinski Hotel, China, July 25, 2018.
  21. Keiji Numata, 高分子科学を利用して植物科学の可能性を広げる. 高分子学会講演会、東工大蔵前会館、2018年7月18日.
  22. Ryoko Hiroyama, Yuka Hatanaka, Taku Demura, Misato Ohtani, snRNA cap hypermethylation: a new key regulatory step of gene expression for plant development and environmental response. 第59回日本植物生理学会年会、札幌コンベンションセンター、2018年3月18日.
  23. Keiji Numata, Rationally-designed polypeptides based on nature and polymer science. Symposium for the 50th Anniversary of Polymer Journal, Nagoya Congress Center, Japan, May 23, 2018.
  24. Keiji Numata, Rationally-designed polypeptides based on structural proteins. 111 Center Symposium, Qingdao, China, April 23-26, 2018.
  25. Keiji Numata、Polypeptide-based micelles to modify plant cells. 日本化学会第98春季年会アジア国際化学シンポジウムナノテク・材料化学ディビジョン、日本大学理工学部 船橋キャンパス、2018年3月20-23日.
  26. 児玉豊、植物の蛍光イメージング:タイムゲート法で葉緑体自家蛍光を消す.第65回日本生態学会大会、自由集会「植物生理生態学の“見える化”」、 札幌コンベンションセンター、2018年3月14日.
  27. 児玉豊、低温誘導性の葉緑体運動:青色光受容体フォトトロピンは温度受容体である.名古屋大学遺伝子実験施設セミナー、名古屋大学、2018年2月28日.
  28. Keiji Numata, Potential of polypeptide-based materials: from artificial spider silk to gene delivery system. Department Lecture, Universität Bayreuth, Germany, January 18, 2018.
  29. Yutaka Kodama, Phototropin in temperature-dependent chloroplast relocation movement. International Symposium on Plant Photobiology 2018, Shimane Prefectural Convention Center, Japan, January 18, 2018.
  30. 大谷美沙都、植物の高い器官再生能力を支える分子機構〜あらたなクローン増殖技術の展開を探る〜. 岡山大学資源植物科学研究所 作物イノベーション研究ワークショップ、岡山大学、2018年1月18日.
  31. Keiji Numata, Chemoenzymatic synthesis of structural and functional polypeptides. Department Seminar, Royal Institute of Technology (KTH), Sweden, January 15, 2018.
  32. Keiji Numata, Polypeptides as structural and functional materials. HPJ異分野融合ワークショップFrontiers in materials, sensors and devices for humanophilic innovation, 奈良先端科学技術大学院大学, November 20, 2017.
  33. 大谷美沙都, 植物の分化全能性研究から見えてきたRNA代謝と転写のクロストーク. ConBio2017シンポジウム「富澤純一先生メモリアル ―分子生物学の原点から未来は見えるか―」. 神戸ポートアイランド、2017年12月7日.
  34. Yutaka Kodama, Phototropin as thermosensor for chloroplast cold-avoidance response in Marchantia polymorpha. The 65th NIBB Conference/International Marchantia Workshop 2017, Okazaki Conference Center, Japan, December 17, 2017.
  35. Yutaka Kodama, Time gating of chloroplast autofluorescence. The 65th NIBB Conference/International Marchantia Workshop 2017, Okazaki Conference Center, Japan, December 16, 2017.
  36. Yutaka Kodama, Cold-induced chloroplast relocation movement mediated by phototropin. 8th Asia and Oceania Conference on Photobiology, Imperial Palace Seoul Hotel, Korea, November 15, 2017.
  37. 今井拓人、児玉豊、沼田圭司、プロトプラストを用いた植物ミトコンドリアの運動性観察. 植物電顕若手ワークショップ、理研横浜キャンパス、2017年11月15日.
  38. Keiji Numata, Potential of polypeptide-based materials: from spider silk to gene delivery. Department seminar, Deakin University, Australia, November 7th, 2017.
  39. Keiji Numata, Potential of polypeptide-based materials: from spider silk to gene delivery. Evolution & Ecology Research Centre Seminar, University of New South Wales, Australia, November 3rd, 2017.
  40. 沼田圭司、ポリペプチドと高分子科学〜オルガネラ工学から人工クモ糸まで〜. IGERグリーン自然科学レクチャー、名古屋大学リーディング大学院グリーン自然科学国際教育研究プログラム、2017年10月5日.
  41. 大谷美沙都、植物細胞が分裂をためらう理由~脱分化を制御するRNA代謝と転写のカップリング制御~. 日本植物学会第81回大会、東京理科大学、2017年9月8日.
  42. Kousuke Tsuchiya, Keiji Numata. Chemoenzymatic polymerization of unnatural amino acids. 254th ACS National Meeting, Green Polymer Chemistry: Biobased Materials & Biocatalyst, Washington Convention Center, United States, August 20-24, 2017.
  43. 児玉豊、植物の蛍光イメージング技術に関する研究. 第35回日本植物細胞分子生物学会、大宮ソニックシティ、2017年8月30日.
  44. 沼田圭司、ペプチドの化学酵素合成と材料設計〜オルガネラ工学からクモ糸まで〜. 若手ペプチド夏の勉強会、長崎スカイホテル、2017年8月5-7日.
  45. Keiji Numata, Potential of peptide-based materials: from spider silk to gene delivery. Beihang Unversity, China, June 23rd, 2017.
  46. 児玉豊, タイムゲート法:時間をずらして葉緑体の自家蛍光を消す. 第58回日本植物生理学会年会、鹿児島大学、2017年3月16日.
  47. Keiji Numata, Peptide-based transformation technologies for plant organelles. Gordon Research Conference 2017 “Chloroplast Biotechnology”, Ventura Beach Marriott, United States, January 8-13, 2017.
  48. 児玉豊, タイムゲート法:時間をずらして葉緑体の自家蛍光を消す. 第6回オプトバイオシンポジウム, 宇都宮大学, 2016年12月2日.
  49. 児玉豊, Tracking organelle moving in plant cell, 第39回日本分子生物学会, パシフィコ横浜, 2016年12月1日.
  50. 沼田圭司, ゲノム編集技術をいかに植物に利用するか. 日本農業経営大学校 特別講義, 日本農業経営大学校、2016年12月8日.
  51. Keiji Numata, Gene/protein delivery using peptide-based carriers to edit organellar and nuclear genomes. 2016 International Symposium on Plant Transformation Biotechnologies, Institute of Plant and Microbial Biology (IPMB), Academia Sinica, Taipei, October 17-19, 2016.