Accomplishments

Original Papers

  1. Yuta Fujii, Ayaka Yoshimura, Yutaka Kodama, A novel orange-colored bimolecular fluorescence complementation (BiFC) assay using monomeric Kusabira-Orange protein. BioTechniques, In press.
  2. Misato Ohtani, Harunori Kawabe, Taku Demura, Evidence that thiol-based r edox state is critical for xylem vessel cell differentiation. Plant Signaling Behavior, 13, e1428512, 2018.
  3. Takeshi Yoshizumi, Kazusato Oikawa, Jo-Ann Chuah, Yutaka Kodama, Keiji Numata. Selective gene delivery for integrating exogenous DNA into plastid and mitochondrial genomes using peptide-DNA complexes. Biomacromolecules, 19, 5, pp1582–1591, 2018.
  4. Jo-Ann Chuah, Keiji Numata, Stimulus-Responsive Peptide for Effective Delivery and Release of DNA in Plants. Biomacromolecules, 19, 4, pp1154-1163, 2018.
  5. Masahiro Noguchi, Masayuki Fujiwara, Ryosuke Sano, Yoshimi Nakano, Yoichiro Fukao, Misato Ohtani, Taku Demura, Proteomic analysis of xylem vessel cell differentiation in VND7-inducible tobacco BY-2 cells by two-dimensional gel electrophoresis. Plant Biotechnol, 35, 1, pp31-37, 2018.
  6. Tian Tian Tan, Hitoshi Endo, Ryosuke Sano, Kurata Tetsuya, Masatoshi Yamaguchi, Ohtani Misato, Demura Taku, Transcription factors VND1-VND3 contribute to cotyledon xylem vessel formation, Plant Physiol, 176,1, pp773-789, 2018.
  7. Harunori Kawabe, Misato Ohtani, Tetsuya Kurata, Tomoaki Sakamoto, Taku Demura, Protein S-nitrosylation regulates xylem vessel cell differentiation in Arabidopsis. Plant Cell Physiol, 59, 1, pp17-29, 2018.
  8. Hiroyuki Tanaka, Mayuko Sato, Yuka Ogasawara, Noriko Hamashima, Othmar Buchner, Andreas Holzinger, Kiminori Toyooka, Yutaka Kodama. Chloroplast aggregation during the cold-positioning response in the liverwort Marchantia polymorpha L. Journal of Plant Research, 130, 6, pp1061-1070. Nov 2017.
  9. Yasuhide Osaki, Yutaka Kodama, Particle bombardment and subcellular protein localization analysis in the aquatic plant Egeria densa. PeerJ, 5, e3779, 2017.
  10. Kousuke Tsuchiya, Keiji Numata, Chemoenzymatic synthesis of polypeptides containing the unnatural amino acid 2-aminoisobutyric acid. Chemical Communications, 53, pp7318-7321, 2017.
  11. Yuta Fujii,Hiroyuki Tanaka,Naotake Konno,Yuka Ogasawara,Noriko Hamashima,Saori Tamura,Satoshi Hasegawa,Yoshio Hayasaki,Koji Okajima,Yutaka Kodama, Phototropin perceives temperature based on the lifetime of its photoactivated state, Proc Natl Acad Sci U S A, 114, 34 :9206–9211, 2017.
  12. Takeshi Yoshizumi, Miwa Yamada, Mieko Higuchi-Takeuchi, Ken’ichiro Matsumoto, Seiichi Taguchi, Minami Matsui, Keiji Numata, Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants. Plant Biotechnology, 34, 1, pp39-43, 2017.
  13. Jo-Ann Chuah, Yoko Horii, Keiji Numata, Peptide-Derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants. Journal of Visualized Experiments (JoVE), 118, e54972, 2016.

Review(peer-reviewed)

  1. Yuta Fujii, Yutaka Kodama. Refinements to light sources used to analyze the chloroplast cold-avoidance response over the past century (Invited article addendum). Plant Signaling Behavior, In press.
  2. Misato Ohtani. Plant snRNP biogenesis: a perspective from the nucleolus and Cajal bodies, Front Plant Sci,8,2184, 2018
  3. Kosuke Tsuchiya, Keiji Numata. Chemoenzymatic synthesis of polypeptides as functional and structural materials (Invited review). Macromolecular Bioscience, 1700177, 2017

Review & notes(domestic)

  1. 沼田圭司. 「ペプチド法:人工制限酵素をはじめとしたタンパク質の導入技術として」、植物化学調節学会、植物の生長調節2017年5月、Vol52、No1.

Invited lecture & seminar

  1. Keiji Numata, Molecular design and biosynthesis of structural proteins based on their hierarchical structures in nature. Beihang Unversity, China, June 5, 2018.
  2. Keiji Numata, Rationally-designed polypeptides based on nature and polymer science. Symposium for the 50th Anniversary of Polymer Journal, Nagoya Congress Center, Japan, May 23, 2018.
  3. Keiji Numata, Rationally-designed polypeptides based on structural proteins. 111 Center Symposium, Qingdao, China, April 23-26, 2018.
  4. Keiji Numata、Polypeptide-based micelles to modify plant cells. 日本化学会第98春季年会アジア国際化学シンポジウムナノテク・材料化学ディビジョン、日本大学理工学部 船橋キャンパス、2018年3月20-23日.
  5. 児玉豊、植物の蛍光イメージング:タイムゲート法で葉緑体自家蛍光を消す.第65回日本生態学会大会、自由集会「植物生理生態学の“見える化”」、 札幌コンベンションセンター、2018年3月14日.
  6. 児玉豊、低温誘導性の葉緑体運動:青色光受容体フォトトロピンは温度受容体である.名古屋大学遺伝子実験施設セミナー、名古屋大学、2018年2月28日.
  7. Keiji Numata, Potential of polypeptide-based materials: from artificial spider silk to gene delivery system. Department Lecture, Universität Bayreuth, Germany, January 18, 2018.
  8. Yutaka Kodama, Phototropin in temperature-dependent chloroplast relocation movement. International Symposium on Plant Photobiology 2018, Shimane Prefectural Convention Center, Japan, January 18, 2018.
  9. 大谷美沙都、植物の高い器官再生能力を支える分子機構〜あらたなクローン増殖技術の展開を探る〜. 岡山大学資源植物科学研究所 作物イノベーション研究ワークショップ、岡山大学、2018年1月18日.
  10. Keiji Numata, Chemoenzymatic synthesis of structural and functional polypeptides. Department Seminar, Royal Institute of Technology (KTH), Sweden, January 15, 2018.
  11. Keiji Numata, Polypeptides as structural and functional materials. HPJ異分野融合ワークショップFrontiers in materials, sensors and devices for humanophilic innovation, 奈良先端科学技術大学院大学, November 20, 2017.
  12. 大谷美沙都, 植物の分化全能性研究から見えてきたRNA代謝と転写のクロストーク. ConBio2017シンポジウム「富澤純一先生メモリアル ―分子生物学の原点から未来は見えるか―」. 神戸ポートアイランド、2017年12月7日.
  13. Yutaka Kodama, Phototropin as thermosensor for chloroplast cold-avoidance response in Marchantia polymorpha. The 65th NIBB Conference/International Marchantia Workshop 2017, Okazaki Conference Center, Japan, December 17, 2017.
  14. Yutaka Kodama, Time gating of chloroplast autofluorescence. The 65th NIBB Conference/International Marchantia Workshop 2017, Okazaki Conference Center, Japan, December 16, 2017.
  15. Yutaka Kodama, Cold-induced chloroplast relocation movement mediated by phototropin. 8th Asia and Oceania Conference on Photobiology, Imperial Palace Seoul Hotel, Korea, November 15, 2017.
  16. 今井拓人、児玉豊、沼田圭司、プロトプラストを用いた植物ミトコンドリアの運動性観察. 植物電顕若手ワークショップ、理研横浜キャンパス、2017年11月15日.
  17. Keiji Numata, Potential of polypeptide-based materials: from spider silk to gene delivery. Department seminar, Deakin University, Australia, November 7th, 2017.
  18. Keiji Numata, Potential of polypeptide-based materials: from spider silk to gene delivery. Evolution & Ecology Research Centre Seminar, University of New South Wales, Australia, November 3rd, 2017.
  19. 沼田圭司、ポリペプチドと高分子科学〜オルガネラ工学から人工クモ糸まで〜. IGERグリーン自然科学レクチャー、名古屋大学リーディング大学院グリーン自然科学国際教育研究プログラム、2017年10月5日.
  20. 大谷美沙都、植物細胞が分裂をためらう理由~脱分化を制御するRNA代謝と転写のカップリング制御~. 日本植物学会第81回大会、東京理科大学、2017年9月8日.
  21. Kousuke Tsuchiya, Keiji Numata. Chemoenzymatic polymerization of unnatural amino acids. 254th ACS National Meeting, Green Polymer Chemistry: Biobased Materials & Biocatalyst, Washington Convention Center, United States, August 20-24, 2017.
  22. 児玉豊、植物の蛍光イメージング技術に関する研究. 第35回日本植物細胞分子生物学会、大宮ソニックシティ、2017年8月30日.
  23. 沼田圭司、ペプチドの化学酵素合成と材料設計〜オルガネラ工学からクモ糸まで〜. 若手ペプチド夏の勉強会、長崎スカイホテル、2017年8月5-7日.
  24. Keiji Numata, Potential of peptide-based materials: from spider silk to gene delivery. Beihang Unversity, China, June 23rd, 2017.
  25. 児玉豊, タイムゲート法:時間をずらして葉緑体の自家蛍光を消す. 第58回日本植物生理学会年会、鹿児島大学、2017年3月16日.
  26. Keiji Numata, Peptide-based transformation technologies for plant organelles. Gordon Research Conference 2017 “Chloroplast Biotechnology”, Ventura Beach Marriott, United States, January 8-13, 2017.
  27. 児玉豊, タイムゲート法:時間をずらして葉緑体の自家蛍光を消す. 第6回オプトバイオシンポジウム, 宇都宮大学, 2016年12月2日.
  28. 児玉豊, Tracking organelle moving in plant cell, 第39回日本分子生物学会, パシフィコ横浜, 2016年12月1日.
  29. 沼田圭司, ゲノム編集技術をいかに植物に利用するか. 日本農業経営大学校 特別講義, 日本農業経営大学校、2016年12月8日.
  30. Keiji Numata, Gene/protein delivery using peptide-based carriers to edit organellar and nuclear genomes. 2016 International Symposium on Plant Transformation Biotechnologies, Institute of Plant and Microbial Biology (IPMB), Academia Sinica, Taipei, October 17-19, 2016.