A hands-on workshop on various areas of structural biology including membrane protein crystallography is scheduled from 19th to 21st of October, 2006. Research Director Prof. So Iwata is the organiser of:
Course II: High Throughput Overexpression, Purification and Crystallization of Membrane Proteins
The course is to be offered on the second day of the workshop. Please join us and get all your burning questions answered by the expert.
In the workshop, we will address the latest progress in the high throughput crystallography on membrane proteins. In the morning session, we will have three short talks on (i) membrane protein expression using GFP fusions (ii) expression of G-Protein coupled receptors and (iii) crystallisation methods in membrane proteins. In the afternoon, we will have a practical session on the fast detection of membrane protein expression level using the whole cell and in-gel GFP fluorescence. Experts of membrane protein expression, purification and crystallisation from Imperial College London and ERATO Human Receptor Crystallography project will support the practice of the course tutees and answer their questions.
The course material for the practical session is adapted from a popular paper published on Nature Method this April, and handled by its first author. (2006.9.21)
Prof. Ray Stevens of Scripps Research Institute kindly agreed to have a presentation on their Joint Center for Innovative Membrane Protein Technologies (JCIMPT). Please note the the course will start 10 minutes earlier than previously announced to accommodate the change. (2006.8.22)
| 9:50 | So Iwata (Imperial College London, Diamond Light Source, ERATO HRC project) | Introduction |
| 10:00 | David Drew (Imperial College London) | Optimization of Membrane Protein Overexpression and Purification Using GFP Fusions |
| 10:30 | Takuya Kobayashi (ERATO HRC project) | Expression and Purification of G-protein Coupled Receptors |
| 11:00 | Takeshi Murata (ERATO HRC project) | Crystallisation of Membrane Proteins |
| 11:30 | Ray Stevens (Scripps Research Institute) | The JCIMPT - Innovative Technologies and Methods for Membrane Protein Structural Biology |
| 12:00 | Lunch | |
| 13:30-17:30 | Practical session “Fast Detection of Membrane Protein Expression Level Using the Whole Cell and In-gel GFP Fluorescence” |
For the further details such as other courses offered at the workshop, please visit the workshop HP. If you have any question, please contact the workshop secretariat.