研究成果

2017

Seo, N., Shirakura, Y., Tahara, Y., Momose, F., Harada, N., Ikeda, H., Akiyoshi, K. & Shiku, H.Activated CD8(+) T cell extracellular vesicles prevent tumour progression by targeting of lesional mesenchymal cells. Nature Communications, 9 , Doi:10.1038/s41467-018-02865-1(2018).

Nishimura, T., Yamada, A., Umezaki, K., Sawada, S., Mukai, S., Sasaki, Y. & Akiyoshi, K.Self-Assembled Polypeptide Nanogels with Enzymatically Transformable Surface as a Small Interfering RNA Delivery Platform.Biomacromolecules, 18, 3913-3923, Doi:10.1021/acs.biomac.7b00937(2017).

Nishimura, T., Sasaki, Y. & Akiyoshi, K. Biotransporting Self-Assembled Nanofactories Using Polymer Vesicles with Molecular Permeability for Enzyme Prodrug Cancer Therapy. Adv. Mater. 29, Doi:10.1002/adma.201702406(2017).

Shimoda, A., Tahara, Y., Sawada, S., Sasaki, Y. & Akiyoshi, K. Glycan profiling analysis using evanescent-field fluorescence-assisted lectin array: Importance of sugar recognition for cellular uptake of exosomes from mesenchymal stem cells. Biochem. Biophys. Res. Commun. 491, 701-707, Doi:10.1016/j.bbrc.2017.07. 126(2017).

Kawasaki, R., Sasaki, Y. & Akiyoshi, K. Self-assembled Nanogels of Carborane-bearing Polysaccharides for Boron Neutron Capture Therapy. Chem. Lett. 46, 513-515, Doi:10.1246/cl.161137(2017).

Maeda, H., Kobayashi, H., Miyahara, T., Hashimoto, Y., Akiyoshi, K. & Kasugai, S. Effects of a polysaccharide nanogel-crosslinked membrane on wound healing. J. Biomed. Mater. Res. Part B 105, 544-550, Doi:10.1002/jbm.b.33571(2017).

Sawada, S., Yukawa, H., Takeda, S., Sasaki, Y. & Akiyoshi, K. Self-assembled nanogel of cholesterol-bearing xyloglucan as a drug delivery nanocarrier.J.Biomater.Sci.-Polym. Ed., 281183-1198, Doi:10.1080/09205063.2017.1320827(2017).

Kawasaki, R., Sasaki, Y. & Akiyoshi, K.Intracellular delivery and passive tumor targeting of a self-assembled nanogel containing carborane clusters for boron neutron capture therapy. Biochem. Biophys. Res. Commun. 483, 147-152, Doi:10.1016/j.bbrc.2016.12.176(2017).

2016

Sekine, Y., Endo, H., Iwase, H., Takeda, S., Mukai, S., Fukazawa, H., Littrell, KC., Sasaki,Y. & Akiyoshi, K. Nanoscopic Structural Investigation of Physically Cross-Linked Nanogels Formed from Self-Associating Polymers. Journal of Physical Chemistry B 120,11996-12002, Doi:10.1021/acs.jpcb.6b06795(2016).

・Nishimura, T. & Akiyoshi, K. Amylose engineering: phosphorylase-catalyzed polymerization of functional saccharide primers for glycobiomaterials. WIREs Nanomedicine and Nanobiotechnology , Doi:10.1002/wnan.1423.

・Tahara, Y., Sakiyama, M., Takeda, S., Nishimura, T., Mukai, S., Sawada, S., Sasaki, Y. & Akiyoshi, K. Self-assembled nanogels of cholesterol-bearing hydroxypropyl cellulose: A thermoresponsive building block for nanogel tectonic materials. Langmuir , Doi:10.1021/acs.langmuir.6b02406(2016).

・Kamiya, K., Kawano, R., Osaki, T., Akiyoshi, K. & Takeuchi, S. Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes. Nature Chemistry 8, 881–889, Doi:10.1038/nchem.2537(2016).

・Kawasaki, R., Sasaki, Y., Katagiri, K., Mukai, S., Sawada, S. & Akiyoshi, K. Magnetically Guided Protein Transduction by Hybrid Nanogel Chaperones with Iron Oxide Nanoparticles. Angew. Chem. Int. Ed. 55, 11377-81, Doi:10.1002/anie.201602577(2016).

・Momose, F., Seo, N., Akahori, Y., Sawada, S., Harada, N., Ogura, T., Akiyoshi, K. & Shiku, H. Guanine- Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types. PLOS ONE 11, e0154134, Doi:10.1371/journal.pone.0154134 (2016).

・Shimoda, A., Chen, Y. & Akiyoshi, K. Nanogel containing electrospun nanofibers as a platform for stable loading of proteins. RSC Adv. 6, 40811-40817, Doi:10.1039/C6RA05997J (2016).

・甲田優太、佐々木善浩、秋吉一成。会合性グラフト高分子の自己組織化によるナノゲル形成と機能。高分子論文集Vol. 73, No. 2, 166-174(2016)。

・Hashimoto, Y., Mukai, S., Sawada, S., Sasaki, Y. & Akiyoshi, K. Advanced Artificial Extracellular Matrices Using Amphiphilic Nanogel-Cross-Linked Thin Films To Anchor Adhesion Proteins and Cytokines. ACS Biomater. Sci. Eng. 2, 375-384, Doi: 10.1021/acsbiomaterials.5b00485 (2016).

・Sato, YT., Umezaki, K., Sawada, S., Mukai, S., Sasaki, Y., Harada, N., Shiku, H. & Akiyoshi, K. Engineering hybrid exosomes by membrane fusion with liposomes. Scientific Reports 6, Doi:10.1038/srep21933 (2016).

・Shimoda, A., Ueda, K., Nishiumi, S., Murata-Kamiya, N., Mukai, S., Sawada, S., Azuma, T., Hatakeyama, M. & Akiyoshi, K. Exosomes as nanocarriers for systemic delivery of the Helicobacter pylori virulence factor CagA. Scientific Reports 6, Doi:10.1038/srep18346 (2016).

・Nishimura, T., Takara, M., Mukai, S., Sawada, S., Sasaki, Y. & Akiyoshi, K. A Light Sensitive Self-Assembled Nanogel as a Tecton for Protein Patterning Materials. Chemical Communications 52, 1222-1225, Doi:10.1039/C5CC08416D (2016).

・Ando, M., Akiyama, M., Okuno, D., Hirano, M., Ide, T., Sawada, S., Sasaki, Y. & Akiyoshi, K. Liposome chaperon in cell-free membrane protein synthesis: One-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method. Biomaterials Science 4, 258-264, Doi:10.1039/C5BM00285K (2016).

2015

・Niwa, T., Sasaki, Y., Uemura, E., Nakamura, S., Akiyama, M., Ando, M., Sawada, S., Mukai, S., Ueda, T., Taguchi, H. & Akiyoshi, K. Comprehensive study of liposomeassisted synthesis of membrane proteins using a reconstituted cell-free translation system. Scientific Reports 5, Doi:10.1038/srep18025 (2015).

・Chan, M., Lux, J., Nishimura, T., Akiyoshi, K. & Almutairi, A. Long-Lasting and Efficient Tumor Imaging Using a High Relaxivity Polysaccharide Nanogel Magnetic Resonance Imaging Contrast Agent. Biomacromolecules 16(9) ,2964-2971, Doi:10.1021/acs.biomac.5b00867 (2015).

・Nishimura, T., Umezaki, K., Mukai, S., Sawada, S. & Akiyoshi, K. Amylose-Based Cationic Star Polymers for siRNA Delivery. BioMed Research International, Doi:10.1155/2015/962941 (2015).

・Takahashi, H., Akiyoshi, K. & Kuroda, K. Affinity-mediated capture and release of amphiphilic copolymers for controlling antimicrobial activity. Chemi. Commun. 51, 12597-12600, Doi:10.1039/C5CC02012C (2015).

・Ogawa, R., Nagao, K., Taniuchi, K., Tsuchiya, M., Kato, U., Hara, Y., Inaba, T., Kobayashi, T., Sasaki, Y., Akiyoshi, K., Watanabe-Takahashi, M., Nishikawa, K. & Umeda, M. Development of a Novel Tetravalent Synthetic Peptide That Binds to Phosphatidic Acid. PLOS ONE 10, e0131668, Doi:10.1371/Journal.pone.0131668 (2015).

・Tahara, Y., Mukai, S., Sawada, S., Sasaki, Y. & Akiyoshi, K. Nanocarrier-integrated microspheres:Nanogel tectonic engineering for advanced drug delivery systems. Advanced Materials 27, 5080-8, Doi:10.1002/adma.201501557 (2015).

・Tahara, Y. & Akiyoshi, K. Current advances in self-assembled nanogel delivery systems for immunotherapy. Adv. Drug Deliv. Rev. 95, 65-76, Doi:10.1016/j.addr.2015.10.004 (2015).

・Fukuyama, Y., Yuki, Y., Katakai, Y., Harada, N., Takahashi, H., Takeda, S., Mejima, M., Joo, S., Kurokawa, S., Sawada, S., Shibata, H., Park, E. J., Fujihashi, K., Briles, D. E., Yasutomi, Y., Tsukada, H., Akiyoshi, K. & Kiyono, H. Nanogel-based pneumococcal surface protein A nasal vaccine induces microRNA-associated Th17 cell responses with neutralizing antibodies against Streptococcus pneumoniae in macaques. Mucosal Immunol 8, 1144-53, Doi:10.1038/mi.2015.5 (2015).

・Nishimura, T., Mukai, S., Sawada, S. & Akiyoshi, K. Glyco Star Polymers as Helical Multivalent Host and Biofunctional Nano-Platform. ACS Macro Letters 4, 367-371, Doi:10.1021/acsmacrolett.5b00049 (2015).

・Sato, T., Alles, N., Khan, M., Nagano, K., Takahashi, M., Tamura, Y., Shimoda, A., Ohya, K., Akiyoshi, K. & Aoki, K. Nanogel-crosslinked nanoparticles increase the inhibitory effects of W9 synthetic peptide on bone loss in a murine bone resorption model. Int. J. Nanomed. 10, 3459-73, Doi:10.2147/IJN.S61566 (2015).

・Tsuchido, Y., Sakai, Y., Aimu, K., Hashimoto, Ｔ., Akiyoshi, Ｋ. & Hayashita, Ｔ. The design of phenylboronic acid azoprobe-polyamidoamine dendrimer complexes as supramolecular sensors for saccharide recognition in water. New J. Chem. 39, 2620-2626, Doi:10.1039/C4NJ01309C (2015).

・Tsuchido, Y., Sasaki, Y., Sawada, S. & Akiyoshi, K. Protein nanogelation using vitamin B6-bearing pullulan : effect of zinc ions. Polymer Journal 47, 201-205, Doi:10.1038/pj.2014.120 (2015).

・Hashimoto, Y., Mukai, S., Sawada, S., Sasaki, Y. & Akiyoshi, K. Nanogel tectonic porous gel loading biologics, nanocarriers, and cells for advanced scaffold. Biomaterials 37, 107-115, Doi:10.1016/j.biomaterials.2014.10.045 (2015).

・Tahara, Y., Yasuoka, J., Sawada, S., Sasaki, Y. & Akiyoshi, K. Effective CpG DNA delivery using amphiphilic cycloamylose nanogels. Biomaterials Science 3, 256-264, Doi:10.1039/C4BM00293H (2015). Cover picture

2014

・Fujii, H.,Shin-Ya, M., Takeda, S., Hashimoto, Y., Mukai, S., Sawada, S., Adachi, T., Akiyoshi, K., Miki, T. & Mazda, O. Cycloamylose‐Nanogel DDS‐mediated intratumor silencing of the vascular endothelial growth factor regulates neovascularization in tumor microenvironment. Cancer science 105, 1616-1625, Doi:10.1111/cas.12547 (2014).

・Katagiri, K., Ohta, K. Sako, K., Inumaru, K., Hayashi, K., Sasaki, Y. & Akiyoshi, K. Development and Potential Theranostic Applications of a Self‐Assembled Hybrid of Magnetic Nanoparticle Clusters with Polysaccharide Nanogels. ChemPlusChem 79, 1631-1637, Doi:10.1002/cplu.201402159 (2014).

・Yokota, M., Kobayashi, Y., Morita, J., Suzuki, H., Hashimoto, Y., Sasaki, Y., Akiyoshi, K. & Moriyama, K. Therapeutic effect of nanogel-based delivery of soluble FGFR2 with S252W mutation on craniosynostosis. PLOS ONE 9, e101693, Doi:10.1371/journal.pone.0101693 (2014).

・Sekine, Y., Okazaki, K., Ikeda-Fukazawa, T., Ichikawa, M., Yoshikawa, K., Mukai, S. & Akiyoshi, K. Microrheology of polysaccharide nanogel-integrated system. Colloid and Polymer Science 292, 325-331, Doi:10.1007/S00396-013-3069-8 (2014).

・Muraoka, D., Harada, N., Hayashi, T., Tahara, Y., Momose, F., Sawada, S., Mukai, S., Akiyosi, K. & Shiku, H. Nanogel-based immunologically stealth vaccine targets macrophages in the medulla of lymph node and induces potent antitumor immunity. ACS Nano 8, 9209-9218, Doi:10.1021/nn502975r (2014).

・Maegawa, Y., Mochizuki, S., Sanada, Y., Akiyoshi, K. & Sakurai, K. Dual-sugar Nanogel Comprising β-1,3-Glucan and Cholesterol-bearing Dextran for Protein Delivery Targeting Antigen Presenting Cells. Chemistry Letters 43, 652-654, Doi:10.1246/Cl.131201 (2014).

2013

・Yuki, Y., Nochi, T.,Kong, IG., Takahashi, H., Sawada, S., Akiyoshi, K. & Kiyono, H. Nanogel-based antigen-delivery system for nasal vaccines. Biotechnol. Genet. Eng. Rev. 29, 61-72, Doi:10.1080/02648725.2013.801226 (2013).

・Tamura, A., Ikeda, G., Seo, JH., Tsuchiya, K., Yajima, H., Sasaki, Y., Akiyoshi, K. & Yui, N. Molecular logistics using cytocleavable polyrotaxanes for the reactivation of enzymes delivered in living cells. Scientific Reports 3, Doi:10.1038/Srep02252 (2013).

・Takeo, M., Mori, T., Niidome, T., Sawada, S., Akiyoshi, K. & Katayama, Y. A polyion complex nanogel. J. Colloid. Interf. Sci. 390, 78-84, Doi:10.1016/j.jcis.2012.09.015 (2013).

・Takeda, S., Takahashi, H., Sawada, S., Sasaki, Y. & Akiyoshi, K. Amphiphilic nanogel of enzymatically synthesized glycogen as an artificial molecular chaperone for effective protein refolding. Rsc Advances 3, 25716-25718, Doi:10.1039/C3ra44572k (2013).

・Takahashi, H., Tahara, Y., Sawada, S. & Akiyoshi, K. Cationic amphiphilic polysaccharide nanoballs: protein stabilization and intracellular delivery by nano-encapsulation. Biomaterials Science 1, 842-849, Doi:10.1039/C3bm00178d (2013).

・Tahara, Y., Kosuge, S., Sawada, S., Sasaki, Y. & Akiyoshi, K. Nanogel bottom-up gel biomaterials for protein delivery: Photopolymerization of an acryloyl-modified polysaccharide nanogel macromonomer. Reactive & Functional Polymers 73, 958-964, Doi:10.1016/J.Reactfunctpolym.2013.02.002 (2013).

・Muraoka, D., Nishikawa, H., Noguchi, T., Wang, L., Harada, N., Sato, E., Luescher, I., Nakayama, E., Kato, T. & Shiku, H. Establishment of animal models to analyze the kinetics and distribution of human tumor antigen-specific CD8(+) T cells. Vaccine 31, 2110-2118, Doi:10.1016/J.Vaccine.2013.02.056 (2013).

・Morimoto, N., Yamazaki, M., Tamada, J. & Akiyoshi, K. Polysaccharide-hair cationic polypeptide nanogels: self-assembly and enzymatic polymerization of amylose primer-modified cholesteryl poly(L-lysine). Langmuir 29, 7509-7514, Doi:10.1021/la3047774 (2013).

・Morimoto, N., Hirano, S., Takahashi, H., Loethen, S., Thompson, DH. & Akiyoshi, K. Self-Assembled pH-Sensitive Cholesteryl Pullulan Nanogel As a Protein Delivery Vehicle. Biomacromolecules 14, 56-63, Doi:10.1021/Bm301286h (2013).

・Kong, IG., Sato, A., Yuki, Y., Nochi, T., Takahashi, H., Sawada, S., Mejima, M., Kurokawa, S., Okada, K., Sato, S., Briles, DE., Kunisawa, J., Inoue, Y., Yamamoto, M., Akiyoshi, K. & Kiyono, H. Nanogel-based PspA intranasal vaccine prevents invasive disease and nasal colonization by Streptococcus pneumoniae. Infect. Immun. 81, 1625-1634, Doi:10.1128/IAI.00240-13 (2013).

・Katagiri, K., Ohta, K., Koumoto, K., Kurosu, K., Sasaki, Y. & Akiyoshi, K. Templated nucleation of hybrid iron oxide nanoparticles on polysaccharide nanogels. Colloid and Polymer Science 291, 1375-1380, Doi:10.1007/S00396-012-2868-7 (2013).

・Kageyama, S., Wada, H., Muro, K., Niwa, Y., Ueda, S., Miyata, H., Takiguchi, S., Sugino, S-H., Miyahara, Y., Ikeda, H., Imai, N., Sato, E., Yamada, T., Osako, M., Ohnishi, M., Hrada, N., Hishida. T., Doki, Y. & Shiku, H. Dose-dependent effects of NY-ESO-1 protein vaccine complexed with cholesteryl pullulan (CHP-NY-ESO-1) on immune responses and survival benefits of esophageal cancer patients. J. Transl. Med. 11, 246, Doi:10.1186/1479-5876-11-246 (2013).

・Hayakawa, E. H., Mochizuki, E., Tsuda, T., Akiyoshi, K., Matsuoka, H. & Kuwabata, S. The effect of hydrophilic ionic liquids 1-ethyl-3-methylimidazolium lactate and choline lactate on lipid vesicle fusion. PLoS One 8, e85467, Doi:10.1371/journal.pone.0085467 (2013).

・Gao, M., Toita, S., Sawada, S., Akiyoshi, K. & Stokke, B. T. Cyclodextrin triggered dimensional changes of polysaccharide nanogel integrated hydrogels at nanometer resolution. Soft Matter 9, 5178-5185, Doi:10.1039/C3sm27662g (2013).

2012

・Shimoda, A., Yamamoto, Y., Sawada, S. & Akiyoshi, K. Biodegradable nanogel-integrated hydrogels for sustained protein delivery. Macromolecular Research 20,266-270, Doi:10.1007/s13233-012-0054-x (2012).

・Shimoda, A., Sawada, S., Kano, A., Maruyama, A., Moquin, A. Winnik, FM. & Akiyoshi, K. Dual crosslinked hydrogel nanoparticles by nanogel bottom-up method for sustained-release delivery. Colloids Surf B Biointerfaces 99, 38-44, Doi:10.1016/j.colsurfb.2011.09.025 (2012).

・Sekine, Y., Moritani, Y., Ikeda-Fukazawa, T., Sasaki, Y. & Akiyoshi, K. A Hybrid Hydrogel Biomaterial by Nanogel Engineering: Bottom-Up Design with Nanogel and Liposome Building Blocks to Develop a Multidrug Delivery System. Advanced Healthcare Materials 1, 722-728, Doi:10.1002/Adhm.201200175 (2012).

・Miyahara, T., Nyan, M., Simoda, A., Yamamoto, Y., Kuroda, S., Shiota, M., Akiyoshi, K. & Kasugai, S. Exploitation of a novel polysaccharide nanogel cross-linking membrane for guided bone regeneration (GBR). J. Tissue Engineering and Regererative Medicine 6, 666-672, Doi:10.1002/Term.475 (2012).

・Fujioka-Kobayashi, M., Ota. M. S., Shimoda, A., Nakahama, K., Akiyoshi, K., Miyamoto, Y. & Iseki, S. Cholesteryl group- and acryloyl group-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering. Biomaterials 33, 7613-7620, Doi:10.1016/J.Biomaterials.2012.06.075 (2012).